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 CCNE Nano-Bio Seminar Series
Presents
Paul Yager, PhD
Professor
Department of Bioengineering
University of Washington
Seattle, WA
Integration of Paper Microfluidic Methods for Detection of
Infectious Diseases for Low Resource Settings
Thursday, September 11, 2014
Seminar & Discussion: 5:30 pm – 6:30 pm
Beckman Building, Munzer Auditorium
http://cmgm.stanford.edu/munzer/location.html
Reception: 6:30 pm – 6:50 pm
Lobby of Munzer Auditorium
Abstract
Two-dimensional paper networks (2DPNs) are a new class of devices that allow complex chemical
processing in a very low-cost format. We have, for the last 6 years, been learning how to translate what we
have learned about point-of-care diagnostic technologies in conventional microfluidics into the language of
porous media. The wicking of fluids in porous materials (like paper, nitrocellulose membranes, etc.) allows
us to discard pumps, which permits great savings in complexity and cost, and the potential to perform
complex tests without any permanent instruments. However, there are many physical and chemical
differences between open ducts and porous media--we have put a good deal of effort into understanding the
performance and design rules of simple paper systems. Currently, the two primary applications for this
technology in our lab are for detection of pathogens that cause human infectious disease: 1) highly-sensitive
multiplexed protein binding assays (of which immunoassays are a class) for detection of influenza, and 2)
ultrasensitive rapid multiplexed isothermal nucleic acid amplification assays for detection of pathogens by
DNA and RNA. All assays are designed to operated by untrained users in low resource settings (e.g. include
the home), and employ visible optical readout that can be captured and quantified using camera-equipped
cellular phones.
Sponsored by: Center for Cancer Nanotechnology Excellence and Translation (CCNE-T) Program NIH/NCI U54
Hosted by: Dr. Sanjiv Sam Gambhir, Departments of Radiology & Bioengineering