Download Scientist, Cellular Immunology

Scientist, Cellular Immunology
121 Bio, LLC, is a start-up based in Cambridge, MA pursuing an innovative protein- and cell-engineering
platform with broad applications in therapeutics, diagnostics, and research tools. The company is
developing an innovative cancer immune cell therapy approach using 121 Bio’s unique platform for
attaching antibodies to cells. This novel approach involves attaching tumor-directed antibodies to a
patient’s immune cells, thereby redirecting the cells to attack tumor cells. The therapy is aimed at
improving on current state of the art cell therapy approaches, providing the ability to redirect cells to attack
tumors, without genetic modification of the cells. The company is initiating an early stage discovery
program directed to obtaining further mechanistic insight, optimization, proof of concept in vitro and in
vivo, and generation of pre-clinical candidates. It will pursue product development through establishment
of an internal pipeline and collaborations with biopharmaceutical companies.
Job Description:
 Play a key role in the discovery and development of novel cellular immunotherapies using immune
cells modified with tumor directed antibodies.
 Clone, express and manipulate recombinant antibodies and fragments directed to tumor antigens.
 Isolate, purify, and characterize mouse and human immune cells.
 Perform enzymatic assays to attach antibodies to the surface of isolated immune cells.
 Design and conduct experiments utilizing antibody-modified cells in in vitro and in vivo models.
 Perform assays systems including, among others, flow cytometry, multiplex ELISA, proliferation
assays, quantitative PCR, reporter gene assays.
 Train and work closely with research associates.
 Analyze data and present results to team.
 Remain informed about new published and unpublished scientific developments and devise creative
solutions to experimental problems.
Qualifications:
 PhD in Immunology, Tumor Biology, or a related field.
 2+ years of postdoctoral experience in an academic or industrial setting.
 The ideal candidate will have extensive hands-on experience with one or more of the following:
o Cell biology, including transfection and tissue culture;
o flow cytometry and immunological phenotyping;
o isolation and manipulation of blood and blood components;
o in vitro and in vivo functional cell-based assays such as T cell and NK cell assays.
 Desirable but not required:
o Experience with in vivo mouse models.
o Experience with adoptive immunotherapy with NK cells, or T cells, including for example
cells modified by chimeric antigen receptor (CAR) or bi-specific T cell engagers (BiTEs).
Send CV with cover message to:
[email protected]