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PIMA COMMUNITY COLLEGE
Effective Term: Full Academic Year 2017/18
BIO
112
Credit Hours: 3.00
Bioscience Laboratory Fundamentals
Lecture Periods: 1.00
Lab Periods: 6.00
Description:
Preparation of students to become lab technicians by introduction of fundamental skills, knowledge, and
attitudes essential to any lab professional. Includes lab safety, documentation, quality control, lab math,
validation and verification of results. Also includes understanding government regulations, biological solution
preparation, assays, biological separations, and growing cells.
Prerequisite(s): MAT 089 complete module 35, or MAT 095, or MAT 097 (or assessment into MAT 151 or higher
on the Math assessment test), and CHM 130IN or higher (or a score of 34 or higher on the CHM 130
assessment test; banner code “CMAS”) or concurrent enrollment in CHM 130IN or higher.
Course Learning Outcomes:
Upon successful completion of the course, the student will be able to:
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Employ the correct mathematical rules of operation, and be able to apply these to the
preparation of reagents and biological solutions.
Demonstrate the use of instrumentation and techniques common to the bioscience laboratory.
Demonstrate the ability to keep a legal scientific notebook compatible with industry standards.
Practice proper laboratory safety.
Demonstrate the ability to properly use and maintain equipment used in bioscience laboratories.
Understand and demonstrate ability to perform biological separations.
Demonstrate ability to grow, transfer, and enumerate bacterial cells.
Show understanding of principles of growing and handling mammalian cells.
Outline:
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Safety
A. Risk assessment
B. Safety-related government web sites
C. Responding to emergencies
D. Working with chemicals
E. Working with biological hazards
Documentation
A. Keeping a laboratory notebook
B. Writing and following a SOP
Measurements
A. Weight and volume measurement
B. Significant figures in measurement
C. pH measurement
Spectrophotometry
A. Absorbance
B. Transmission
Laboratory Mathematics and Solution Preparation
A. Metric System and calculations
B. Percent Solutions/Dilutions
C. Normal/Molar Solutions
D. Buffers
VI.
Assays
A. Quantitative and qualitative assay of DNA
B. Protein assays
C. Enzyme assays
VII. Biological Separation Methods
A. Separation of materials using a centrifuge
B. Agarose gel electrophoresis
C. Introduction to ion exchange chromatography
VIII. Growing Cells
A. Microscopy
B. Aseptic technique
C. Growing bacteria
D. Streak plate technique
E. Gram staining
F. Enumerating colony-forming units
G. Introduction growth of mammalian cells
IX. Quality Control/Quality Assurance