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Prokaryotes Chapter 27 1 Prevalence of Prokaryotes • • • Prokaryotes are the oldest, abundant for over 2 billion years before the appearance of eukaryotes structurally simplest, most abundant forms of life on earth. – 5,000 different kinds currently recognized bacillus coccus spirillum pili - hairlike structures - attachment endospores - resistant to environment 2 Prevalence of Prokaryotes • Prokaryotes versus Eukaryotes – unicellularity – cell size – chromosomes – cell division and recombination – internal compartmentalization – flagella – metabolic diversity 3 Prokaryotic Diversity • • Very early, prokaryotes split into two lines – Archaea and bacteria are as different in structure and metabolism from each other as either is from eukarya. Comparing archaebacteria and bacteria – plasma membranes composed of different lipids – – – cell wall archaebacteria lack peptidoglycan gene translation machinery archaebacteria similar to eukaryotes gene architecture bacteria not interrupted by introns 4 Gram Stain for Bacterial classification Gram-positive - thicker peptidoglycan Gram-negative - thinner peptidoglycan 5 Flagellar Motor flagella – slender protein - locomotion 6 The Cell Interior • • • Internal membranes – invaginated plasma membrane Nucleoid region – lack nucleus - genes encoded with single double-stranded DNA Ribosomes – Prokaryotic ribosomes are smaller than eukaryotic ribosomes, and differ in protein and RNA content. 7 Prokaryotic Variation • Two process create bacterial variation: – mutation spontaneous errors in DNA replication prokaryotic ability to mutate rapidly – genetic recombination occurs by gene transfer from one cell to another by viruses or conjugation 8 Prokaryotic Metabolism • • Autotrophs – obtain carbon from inorganic CO2 photoautotrophs - sunlight chemoautotrophs - inorganic chemicals Heterotrophs – obtain carbon from organic molecules photoheterotrophs - sunlight chemoheterotrophs - organic molecules 9 Benefits of Prokaryotes decomposition nitrogen fixation digestive tract of animals nonpolluting insect control pollutant removal commercial production of antibiotics 10