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What is energy? – ability to do work Cars need energy to move Animals need energy to grow, move, make noise, etc Where do cars get energy? – from burning fuel –Specifically oil and gasoline Chemical Energy is stored in fuels - Burning oil and gasoline release energy in the form of heat and light ; The energy also allows the car to move. - Gasoline is organic. When gasoline reacts with oxygen you get a combustion reaction. The products of ALL combustion reactions are carbon dioxide (CO2) and Water (H20) What is our fuel? - Food! - Specifically Glucose (simple carb) But where does the glucose in our food come from? THE SUN!!! Really? How? • Let go through step by step. 1. Where did your glucose come from? 2. How did the glucose get in the food? 3.How did she get the glucose? 4. How did the glucose get in the grass? 5. Where did the energy to make the glucose come from? SO, long story short, - the chloroplasts take energy from the sun and put it into a glucose molecule (Photosynthesis) - through the food chain, glucose gets passed from organism to organism - eventually mitochondria of some organism takes the energy back out, by breaking down the glucose (Cell respiration) Types of Batteries • Adenosine Triphosphate: ATP – cycles between ATP (high energy) and ADP (low energy) by adding or removing a phosphate Types of Batteries • Electron Carriers: cycle between low energy “empty” form and high energy “full” form by adding or removing electrons. –We can “empty” these electron carriers to charge ATP Types of Batteries –There are three different electron carriers: NADPH; NADH and FADH2 Photosynthesis Let’s start by getting the energy from the sun into the glucose molecule Key terms: • Heterotrophs: Organisms that get food from somewhere else –Examples?? • Autotrophs: organisms that make food for themselves –Photoautotrophs use light energy to make their food • Examples? –Chemoautotrophs use the energy in inorganic compounds to make their food • Examples? • Understanding a little about light is important! Refer to your “Science of light and Color” assignment to help! Photoautotrophs undergo Photosynthesis • So what organelle is in their cells? Granum Thylakoid (plural Stroma Grana) What makes it Green? • Pigment called Chlorophyll (There are two chlorophylls) • A pigment is a substance that absorbs light What color light bounces off chlorophyll? GREEN!!! • Carotenes and xanthophylls*: other plant pigments (orange and yellow) that absorb different wavelengths of sunlight than the chlorophylls do. • When can we see these?? FALL!!! * ZAN-tho-fills Overall Reaction • Photosynthesis requires MANY reactions but we can summarize it with the following reaction 6CO2 + 6H2O C6H12O6 + 6O2 (Glucose) • Like all reactions, photosynthesis needs: • Two Phases of Photosynthesis Light reactions: need light; - uses light energy to “charge” two energy molecules: ATP and NADPH - proteins needed for the reaction are embedded in the thylakoid membrane - Uses: 6H2O produces: 6O2 • Two Phases of Photosynthesis Calvin cycle: doesn’t need light; - Uses the ATP and NADPH “charged” by the light reactions to link CO2 together to build C6H12O6 - Enzymes for the calvin cycle are found in the stroma Reactant: 6CO2 Product: C6H12O6 Putting it Together Glucose will do one of two things: 1. Move to the mitochondria to be converted into ATP through Cell respiration 2. Go through dehydration synthesis to build a big starch chain and be stored for future use Follow the Energy • Where did the energy start? • Where did it go next? • Where is it at the end of photosynthesis? Factors that affect Photosynthesis • Light intensity (how bright/strong) • CO2 levels • Water • Wavelength (color of light) • Temperature: 0 – 35 degrees C Why do each of these affect photosynthesis? Exceptions to the Rules: • Autotrophic Bacteria: –Example: Cyanobacteria (“Blue” bacteria) Exceptions to the Rules: • Autotrophic Protists: –Example: Algae, volvox and Euglena Exceptions to the Rules: • Heterotrophic plants: some plants get food from other organisms –Mistletoe: makes food AND takes sap (high in sugar) from other trees Exceptions to the Rules: Venus Flytrap: traps and digests insects as a food source Exceptions to the Rules: Dodder plant: roots grow into other plants and steals water and glucose How do we get the energy back out? Cell Respiration – a series of chemical reactions that extract energy from glucose to “charge” ADP to make ATP. Starts in the cytoplasm and ends in the mitochondria. How do we get the energy back out? Overall reaction C6H12O6 + 6O2 6CO2 + 6H2O ATP Two types of Cellular Respiration Anaerobic: no free oxygen required - performed by most bacteria - Also by yeast and animal cells when lacking O2 - two steps: Glycolysis, fermentation - makes 2 ATP per glucose Two types of Cellular Respiration • Aerobic: requires FREE oxygen, O2 gas to get ATP from glucose – three steps: glycolysis, krebs, electron transport chain – performed by plants, animals, yeast, protists, fungus. – Generates 36 ATP per glucose Anaerobic step 1: Glycolysis • Occurs in cytoplasm • Summary: split glucose in half to charge a few ATP and NADH • Reaction: Glucose 2 pyruvate • Energy molecules used: 2ATP • Energy molecules made: 4ATP and 2NADH Fermentation • Occurs in cytoplasm • Summary: “empty” the NADH so we can repeat glycolysis with the next glucose –2Pyruvate CO2 and Ethanol (yeast) or - 2Pyruvate Lactic Acid (bacteria and muscle cells) • NO MORE ATP CHARGED! ATP Tallies: Anaerobic: - Glycolysis: used 2, made 4 - Fermentation: used 0, made 0 Total: +2 per glucose Aerobic step 1: Glycolysis • SAME AS STEP 1 OF ANAEROBIC! Krebs • Occurs in mitochondria • Summary: break down pyruvate into CO2; use energy in pyruvate to charge ATP, NADH and FADH2 Krebs Reaction: –2Pyruvate 2Acetyl-CoA + CO2 –2Acetyl-CoA 4CO2 –Energy molecules made: 2ATP + 2FADH + 6NADH –Can bacteria do Krebs?? Electron Transport Chain • Occurs in mitochondria • Summary: Gather up ALL the electron carriers and “empty” them to “charge” lots of ATP Electron Transport Chain • Reaction: –O2 H2O Energy molecules USED: 10 NADH (from krebs and glycolysis) + 2 FADH (from krebs) Energy molecules MADE: 32 ATP ATP Tallies: Aerobic: - Glycolysis: used 2, made 4 - Krebs: used 0, made 2 - ETC: used 0, made 32 Total: +36 ATP per glucose Overall Energy Summary for Aerobic Respiration Reactants (used up/broken down) Products (created/built up) Glucose + 2ATP 2 Pyruvate 2Acetyl-CoA 10NADH + 2FADH2 + 6O2 2 Pyruvate + 4ATP + 2NADH 2CO2 + 2Acetyl-CoA + 2NADH 6NADH + 2FADH2 + 2ATP+ 4CO2 32ATP + 4CO2 + 6H2O What’s Left? Final Reaction for Aerobic Respiration Putting it together: Sun Chloroplast C6H12O6 + O2 CO2 + H2O Mitochondria 36ATP