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DINATEC Diversified Nutri-Agri Technologies Inc. Advanced Living Probiotic & Natural Mycotoxin Binding Technology OVERVIEW Approximately 90 % of the intestinal flora in birds is composed of facultative lactic acid producing bacteria such as lactobacilli, Enterococcus and some strictly anaerobic bacteria. The remaining 10 % are such as Escherichia coli, clostridium, Staphylococcus, Pseudomonas, and others. (FOX, 1988). Poultry grown in intensive production facilities are subjected to many additional types of stressors 1.Transportation from the hatchery facility to the farm 2. High production density and overpopulation 3. Vaccination programs 4. Variations in environmental temperatures 5. Pathogenic, viral, bacterial and toxicogenic field challenges Stressors have a detrimental effect in the normal balance of the intestinal flora resulting in a decreased immune response, (JIN et al., 1997). Also other important factors are affected such as: Increased intestinal infections due to secretion of toxins, Reduced rate of growth, Increased feed conversion, Reduced egg production, Lowered fertility, Diminished hatchability, Qualitative factors are affected as well such as the carcass quality. (bruising as seen here>>>) These problems are further exacerbated by the likely increase of opportunistic pathogenic bacteria, resultant diarrheas and anemic conditions. (FOX, 1988). Research involving plausible solutions to this problem has recently focused attention on the natural defense mechanisms that are normally available to poultry and livestock in general in their own intestinal portal tissue. MYCOLACTOR is one leading and high quality result of such intense research and investigation by DINATEC, (Diversified Nutri-Agri Technologies Inc.,) Mycolactor seeks to restore the negative effects produced via antibiotic treatments, degrade many deleterious types of mycotoxins and bacterial toxins, and to eventually replace the use of antibiotics altogether. The use of probiotics in poultry operations is gaining momentum by the day. The reason and even necessity for this is that probiotics offer a wide array of advantages that poultry operators can not afford to overlook A probiotic should afford the following characteristics 1. That it contribute probiotics which are analogous to the native micro-flora of the organism. 2. That it not produce any deleterious effects nor originate from toxic substances. 3. That the micro-organisms in the probiotic have the ability to adhere easily to the intestinal wall and multiply rapidly. WHAT IS A unique combination of highly effective live colony forming microorganisms consisting of 150 X 106 CFM of: Saccharomyces Cerevisiae, Enterococcus faecium, Lactobacillus acidophilus, Lacobacillus casei, Lactobacillus plantarrum and Lactobacillus brevis placed in media consisting of dried brewers yeast which includes brewers’ grain (barly and wheat), oligosaccharides and cell wall extract. A 100 % natural probiotic. A 100 % natural toxin binder Natural Probiotic and Toxin Binding Technology Why use MYCOLACTOR? ⊳ Producers commonly feed low levels of antibiotics for growth promotion ⊳ The feed industry is looking for alternatives to this practice because many pathogenic microorganisms are becoming resistant antibiotics ⊳ MYCOLACTOR offers a viable, effective and natural alternative to the widespread use of antibiotics. What does MYCOLACTOR do? Provides a source of live, naturally occurring microorganisms as an aid in the amelioration of mycotoxicoses. Maintains a healthy microbial balance in animals. Supplements desirable organisms to newborn animals during incidences of scours at weaning and other diet changes. Helps maintain appetite. Used as part of a health maintenance program. I. How does MYCOLACTOR work? Beneficial bacteria produce a variety of organic acids and other compounds that inhibit the growth of E.coli and other intestinal pathogens. Organic acids also can serve as energy sources to the animal or other beneficial bacteria. Lactic acid bacteria produce antimicrobials that inhibit intestinal pathogens. Beneficial bacteria deconjugate bile salts inhibiting enteric pathogens. II. How does MYCOLACTOR work? Lactic acid bacteria out-compete pathogens for nutrients in the gut.( figure 1) III. How does MYCOLACTOR work? Animals fed lactic acid-producing bacteria have an increase of activity in their immune system, improving the animal’s resistance to bacterial infections. IV. How does MYCOLACTOR work? Beneficial bacteria produce enzymes which breakdown feedstuffs into smaller, more easily absorbed nutrients. Lactic acid bacteria reduce the level of amines in the gut which are toxic. Lactobacillus acidophilus Lactobacillus casei Enterococcus faecium V. How does MYCOLACTOR work? AGAINST MYCOTOXINS Bio-Transformation Bio-Agglutination Bio- Degradation VI. How does MYCOLACTOR work? Bio- Transformation Bio-Transformation is one of four processes involved in toxicokinetics TOXICOKINETICS VI. How does MYCOLACTOR work? Bio- Transformation Two substances with equal toxicity and absorption may differ in hazard depending on the nature of their iotransformation. A substance biotransformed into a more toxic metabolite (bioactivated) is a greater hazard than a substance that is biotransformed into a less toxic or even non-toxic metabolite (detoxified). VI. How does MYCOLACTOR work? Bio- Transformation What is Toxicokinetics? Toxicokinetics is essentially the study of "how a toxic substance such as a mycotoxin gets into a given physiological organism such as abody or a multicellular biological system and what happens to it in that system". It is widely known that many pathogenic bacteria have become immune to many tyoes of antibiotics which are in many instances mycotoxins. Mycolactor has specially selected strains of Lactobacillus, E. faecium, S. cerevisiae and other technologies that Bio-Transform, (detoxify) mycotoxins. Bio- Agglutination VI. How does MYCOLACTOR work? The process by which toxic or pathogenic substances “stick” to long chain olygosacharide molecules Bio- Degradation VI. How does MYCOLACTOR work? Degaradation via enzymatic activity IMPROVES HEALTH AND PERFORMANCE OF YOUR LIVESTOCK OPTIMIZED INTESTINAL FLORA EQUILIBRIUM BENEFITS EXPLANATION Metabolizes substrates into sugars, vitamins, amino acids & proteins making them unavailable to pathogens. Overpowers & outcompetes pathogenic bacteria. Produces lactic acid decreasing intestinal pH, inhibiting pathogenic bacteria growth. Metabolizes bacteriocines which work against pathogenic bacteria. Increases enzymatic activity & absorption in the intestinal tract. Produces vitamins & enzymes that degrade mycotoxins. BENEFITS EXPLANATION, cont. Promotes healthy equilibrium of the intestinal flora & resulting benefits to the animal. Produces lactic acid which elicits an Immune reponse & the production of antibodies & increased phagocytic activity. Physically precludes the possibility for other harmful bacteria to occupy the same space. COMPARATIVE CHART ITEM GENERAL BENTONITES MYCOLACTOR YES NO YES INORGANIC YES ORGANIC BIO-TRANSFORMATION NO YES BIO-AGGLUTINATION NO YES ENZYMATIC BIO-DEGRADATION NO YES VITAMIN BIO-DEGRADATION NO YES BIO-COMPETITIVE NO YES ENHANCES IMMUNE SYSTEM NO YES METABOLIZES BACTERIOCINES NO YES INCREASED ENZYMATIC& ABSORPTION ACTIVITY NO YES RESTORES FLORA NO YES ADSORPTION NATURAL Guaranteed Analysis Each gram of MYCOLACTOR is guaranteed at a minimum of 150 million colony forming units, CFU’s)Saccharomyces Cerevisiae, Enterococcus faecium, Lactobacillus acidophilus, Lacobacillus casei, Lactobacillus plantarrum and Lactobacillus brevis MYCOLACTOR is placed in a media consisting of dried brewers yeast which includes brewers’ grain (barely and wheat), oligosaccharides and cell wall extract. PRODUCT SPECIFICATIONS ANIMAL FEEDS Beef and Dairy cows, poultry, broilers, layers, turkeys, sheep and goats, pigs and horses EFFICACY As a 100 % natural probiotic & toxin binder DOSAGE 1 kg per metric ton of feed STORAGE In dry cool conditions. Keep away from direct sunlight PRECAUTIONS Please see MSDS EXPIRATION One year in original package PACKAGING Powder and liquid* form for top dressing or mixing in feed; water-dispersible powders for top dressing or mixing in milk substitute or water. *(soon available) … for now … We at DINATEC INC,. want to thank you for taking time to view this MYCOLACTOR presentation