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Alfred Day Hershey His best known experiment is Hershey Chase experiment Concerned the replication of genetic structure of viruses 4 Dec. 1908--- 22 May 1997 1934 -1950 Teaching and research at the Department of Bacteriology 1936 Held an instructorship in bacteriology and immunology 1936 - 1939 Work together with Professor J. J. Bronfenbrenner. 1938 Became assistant professor 1942 Became associate professor What is T2 ? •The T2 phage is a strongly toxic bacteriophage and tadpole-shaped virus • small virus that infect only bacteria •Contain 50% DNA and 50% protein Infect E-coli •Contains linear double-stranded DNA tail fibers of the phage attach to the bacterial cell to initiate infection Protein coat of the T2 carries the organ of attachment to bacteria. The infective cycle of T2 in E.coil •Virus attach to the surface of their host cell (a) •Inject the DNA into the cell (b) •DNA accumulate,other genes are transcribed and translated (C) •DNA and protein are assembled into complete virus (d) •The cell breaks and releases its content of virus (e) Hershey Chase Experiment Radioactive Sulphur (35S) non-radioative DNA? Radioactive Phosphorous(35 P) radioactive DNA?
