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Terms and Players Work on as class comes in– groups, alone, books, notes Francois, Elliot, Dani, Kristen, Kelly: What are the basic monomeric subunits of DNA bonds between subunits direction of strand formation description of double helix Spencer, Byron, Christina, Rachel, Anders What are the basic monomeric subunits of RNA bonds between subunits organization of strand Today: Preview for today Synaptic signaling requires protein receptors and peptide- derived signals How do you make a protein? Protein building 101: Transcription and Translation Questions What is the central dogma? How would a neuropeptide get made (in general terms)? What are the basic parts of DNA, RNA, and proteins? What is the difference between hnRNA, mRNA and tRNA? Synthesis overview What carries the information? Polymerization of nucleotides Genes ‘ The entire nucleic acid sequence necessary for the synthesis of a functional polypeptide’ MCB, p285 Components of a Eukaryotic Gene What are the ‘parts’ or regions of a gene? How are they organized? Do genes always encode RNAs? Do genes always encode RNAs that encode proteins? Questions How does RNA polymerase work and what does it make? How does it know where to start and stop? How does a ribosome work and what does it make? How does it know where to start and stop? If the DNA in every cell in your body is the same why don't your adipose (fat) cells secrete epinephrine? If the DNA contains all of the information why doesn't the ribosome just 'read' it? Why have intermediate steps? Looking at RNA ECB2 7.1 Making a cytosolic protein: Step 1--transcribe Genes encode proteins Why use an RNA step? Major steps in process: Initiation, Elongation and Termination animation Initiation DNA-RNA interactions DNA Template strand Complementary RNA strand DNA/RNA Hybrid Watching Transcription EBC2 7.2 Adapted from Life; Purves 6thed http://www.stolaf.edu/people/giannini/flashanimat/molgenetics/transcription.swf http://www.johnkyrk.com/DNAtranscription.html Check out Synthesis overview
