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THE IMPORTANCE OF LABELLING YOUR S(TUFF)
In the Globe and Mail this weekend there was a little anecdote that went as
follows:
PhD student spends three years in tropical rainforest laboriously collecting lizard
excrement for some sort of analysis.
The PhD student stores the excrement in the lab. freezer in an unlabelled plastic bag.
The PhD student returns to rainforest to collect more excrement (apparently this stuff
is difficult to get!)
The PhD student returns home.
Finds out all his excrement has been thrown out in a lab clean-up.
PhD student says his career and life are ruined.
Let this tragedy be a warning to us all.
THE RER/GOLGI SYSTEM
The Nobel Prize in
Physiology or Medicine
1974.
"for their discoveries
concerning the structural
and functional organization
of the cell"
George Palade
INTRACELLULAR PATHWAY OF
SECRETORY PROTEIN TRAFFIC
LYSOSOMES/PEROXISOMES
Christian de Duve
Charles Leblond,
Marian Neutra,
McGill University
Harvard Medical School
ROLE OF THE GOLGI BODY
Palade is credited with being the first one to describe the small
particles that later came to be known as ribosomes.
HOW DID PALADE AND CO-WORKERS ELUCIDATE THE PATHWAY OF
SECRETORY PROTEIN TRAFFIN IN CELLS?
HOW DID NEUTRA AND LEBLOND ELUCIDATE THE ROLE OF THE
GOLGI BODIES IN PPROTEIN SECRETION?
CHOOSE THE BEST CELLS TO WORK WITH!
PALADE AND CO-WORKERS
DIGESTIVE CELLS OF PANCREAS
NEUTRA AND LEBLOND
SMALL INTESTINE
FIBROBLAST
OSTEOCYTE
(1) CHOOSE THE BEST CELLS TO WORK WITH!
PALADE AND CO-WORKERS
DIGESTIVE CELLS OF PANCREAS
NEUTRA AND LEBLOND
SMALL INTESTINE
(2) USE THE BEST TECHNIQUES
MICROAUTORADIOGRAPHY
DUODENUM
DUCT
ZYMOGEN
GRANULES
BASEMENT
LAMINA
ROUGH
ENDOPLASMIC
RETICULUM
ACINAR
CELLS
MITOCHONDRIA
β-PARTICLES
3H
HELIUM + β-PARTICLE
USE THE AMINO ACID LEUCINE IN WHICH AN R-GROUP
1H-ATOM HAS BEEN REPLACED WITH AN 3H-ATOM
SECRETORY VESICLES WITH PROTEIN
MADE DURING THE EXPERIMENT
SECRETORY VESICLES
DECAY TRACK OF ONE β-PARTICLE
www.ncbi.nlm.nih.gov/bookshelf/picrender.fcgi...
PULSE-CHASE EXPERIMENT
Charles Leblond,
Marian Neutra,
McGill University
Harvard Medical School
ROLE OF THE GOLGI BODY
WANTED TO STUDY ROLE OF GOLGI BODY
USED 3H-FUCOSE (A SUGAR) INSTEAD OF 3H- LEUCINE (AN
AMINO ACID)
GOBLET CELLS FROM EPITHELIUM
OF SMALL INTESTINE
GOBLET CELLS OF RAT INTESTINE
THE MECHANISM OF POLYPETIDE IMPORT INTO THE RER
Post-translational import
PAGE 697
CENTRAL
VACUOLE
MELANOSOMES
Co-translational import
P 687
INTEGRAL MEMBRANE
PROTEINS
GUNTHER BLOBEL
SIGNAL HYPOTHESIS