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Tuesday, February 14, 2012 Homework: • QUIZ BLOCK DAY • WORD WALL SHEET DUE BLOCK DAY • All makeup work should be completed during tutorial today. No makeups after chapter quiz… What does this have to do with Biology? Recap of Sally Snowboarder’s Cellular Transport • Oxygen Transport: Simple Diffusion • Carbon Dioxide Transport: Simple Diffusion • Glucose Transport: Facilitated Diffusion • Amino Acid Transport: Active Transport OXYGEN TRANSPORT Simple diffusion CARBON DIOXIDE TRANSPORT Facilitated diffusion GLUCOSE TRANSPORT Carrier protein ATP • MAKE A LOGBOOK ENTRYEntry9: Cell Transport Quiz Practice2/14/12 • Without looking at your logbook/notes— Tell me what this quiz will be about. • What are at least two big questions that you feel sure you will be asked? For each of the entries you just listed– Determine what the main idea(s) are: What was the point of the activity/lab/discussion and what did we learn? • • • • • EGG DEMO— WATER ACROSS A MEMBRANE LAB— DIFFUSION-OSMOSIS— MEMBRANE STRUCTURE— SALLY SNOWBOARDER— SALT/SOLUTE CONCENTRATION IS VERY IMPORTANT BECAUSE A CELL GAINS OR LOSES WATER BASED ON THE INTERNAL/EXTERNAL SALT CONCENTRATION. EXCESSIVE WATER LOSS CAN CAUSE THE CELL TO SHRINK AND DIE. EXCESSIVE WATER GAIN CAUSES THE CELL TO SWELL AND BURST Corn syrup (Karo light) Question: 1a Water Egg whites More concentrated solution OUTSIDE cell More concentrated solution INSIDE cell Same concentration inside and outside of cell Less concentrated solution inside cell Less concentrated solution outside cell (CONTROL) OVERALL WATER MOVED OUT/EGG LOST MASS OVERALL WATER MOVED IN/EGG GAINED MASS WATER MOVED IN AND OUT/MASS STAYED ABOUT THE SAME WATER IN TUBE Question: 1a SUGAR SOLUTION IN BEAKER SUGAR SOLUTION IN TUBE WATER IN BEAKER W S S W More concentrated solution OUTSIDE tube Less concentrated solution inside cell OVERALL WATER MOVED OUT/TUBE LOST MASS More concentrated solution INSIDE tube Less concentrated solution outside tube OVERALL WATER MOVED IN/TUBE GAINED MASS Question: 1a Sugar water in beaker Plain water in cell beginning mass 7.6 g Plain water in cell ending mass 4.3 g Water moved out of the cell into the beaker Question: 1a Plain water in beaker Sugar water in cell beginning mass 5.2g Sugar water in cell ending mass 8.1g Water moved from the beaker into the cell Question: 1b EXPLAIN Osmosis Two factors cause osmosis: 1. A semipermeable membrane allows water molecules to move back and forth through the membrane, but not solute molecules 2. Water molecules “stick” temporarily to any dissolved solute molecules. As a result, water molecules spend more time and accumulate on the side of the membrane with the higher concentration of dissolved solutes. 2a. Movement of molecules through membranes made only of phospholipids (phospholipid bilayer) Molecule name Size Electric charge Able to cross lipid bilayer membranes between phospholipid molecules? (large or small) (nonpolar, polar charge, or strong charge) CO2 Small Nonpolar YES O2 Small Nonpolar YES H2O Small Polar YES glucose Large Polar NO Ion Small Strong charge NO (carbon dioxide) (Yes or No) 2b WATER– THE SOLVENT— THE THING THAT DISSOLVES THE SOLUTE STARCH– THE SOLUTE— THE THING THAT GETS DISSOLVED IN WATER (SOLVENT) Question: 2b Starch in beaker Location of color change shows that only iodine crossed Iodine in cell Question: 2b Iodine Starch IODINE (SMALL MOLECULE) INSIDE STARCH (LARGE MOLECULE) OUTSIDE COLOR CHANGE OBSERVED IN BEAKER Starch Iodine STARCH (LARGE MOLECULE) INSIDE IODINE (SMALL MOLECULE) OUTSIDE COLOR CHANGED OBSERVED IN TUBE Question: 3 DIFFUSION IS THE RANDOM MOVEMENT OF PARTICLES FROM HIGH TO LOW CONCENTRATION 4. . OSMOSIS HAPPENS BECAUSE THE SEMIPERMEABLE MEMBRANE ALLOWS WATER TO CROSS-BUT NOT THE SOLUTE AND ATTRACTION BETWEEN THE SOLUTE AND WATER HOLD IT ON THAT SIDE LONGER—CAUSING WATER TO BUILD UP ON THE SIDE WITH THE HIGHEST CONCENTRATION 5. CONCENTRATION STOPS CHANGING/THE MOLECULES HAVE EVENLY DISTRIBUTED—BUT THAT THEY CONTINUE MOVING RANDOMLY AROUND THE CONTAINER— MOLECULES NEVER STOP MOVING 6a. SOLUTION IS HYPOTONIC TO CELL. MORE WATER MOVES IN Question: 6b SOLUTION IS HYPERTONIC TO CELL. MORE WATER MOVES OUT 6c. SOLUTION AND CELL ARE ISOTONIC. WATER MOVES IN AND OUT EQUALLY 6. CELL IS IN A HYPOTONIC SOLUTION (CELL IS HYPERTONIC) CELL IS IN A HYPERTONIC SOLUTION (CELL IS HYPOTONIC) CELL IS IN AN ISOTONIC SOLUTION Cell membrane structure Water molecules Polar head faces out towards water because of the attraction Nonpolar tails face inward so they are protected from water Water Passive transport Question: 7a Question: 7b Simple diffusion Facilitated diffusion GLUCOSE CO2 Concentration Gradient Carrier protein SMALL, UNCHARGED MOLECULES MOVE THIS WAY LARGER, UNCHARGED MOLECULES MOVE THIS WAY Question: 7c Active transport MOVEMENT IS LOW TO HIGH CONCENTRATION THIS PROCESS REQUIRES ENERGY (ATP) Concentration Gradient LARGE OR CHARGED MOLECULES MOVE THIS WAY Calcium ion (Ca+) ATP Carrier protein • Question: 8 SIMPLE DIFFUSION FACILITATED DIFFUSION ACTIVE TRANSPORT HIGH TO LOW CONCENTRATION HIGH TO LOW CONCENTRATION LOW TO HIGH CONCENTRATION (MORE TO LESS) (MORE TO LESS) (LESS TO MORE) SMALL, UNCHARGED MOLECULES DIRECTLY ACROSS THE MEMBRANE BILAYER BIG/LARGE, UNCHARGED MOLECULES REQUIRES A PROTEIN CHANNEL/CARRIER BIG/LARGE, CHARGED MOLECULES REQUIRES A PROTEIN CHANNEL/CARRIER NO ENERGY REQUIRED NO ENERGY REQUIRED ENERGY REQUIRED PASSIVE PASSIVE ACTIVE