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3 parts of Respiration Glycolysis – may be anaerobic TCA – Kreb’s Cycle aerobic – require oxygen Electron Transport Chain Electron shuttles span membrane CYTOSOL MITOCHONDRION 2 NADH or 2 FADH2 2 NADH 2 NADH Glycolysis 2 Pyruvate Glucose 2 Acetyl CoA 6 NADH Citric acid cycle + 2 ATP + 2 ATP by substrate-level phosphorylation by substrate-level phosphorylation Maximum per glucose: About 36 or 38 ATP 2 FADH2 Oxidative phosphorylation: electron transport and chemiosmosis + about 32 or 34 ATP by oxidative phosphorylation, depending on which shuttle transports electrons from NADH in cytosol Glycolysis Occurs in the cytoplasm (cytosol) Refer to handout… Glycolysis Glycolysis 1. Phosphorylate glucose – 2 ATP used up Glycolysis 2. Rearrange molecule to form fructose Glycolysis 3. Phosphorylate again – ATP used up Glycolysis 4. Split into two 3-carbon pieces, each containing one P 2 glyceraldehyde 3-phosphate (G3P) or PGAL Glycolysis 5. Add a high-energy phosphate (~ P ) to each piece. NAD+ is reduced to NADH + H Glycolysis 6. Transfer the high-energy phosphate to ADP, making ATP (2 are made)… substrate-level phosphorylation! Glycolysis 7. Rearrange the position of the remaining phosphate Glycolysis 8. Remove H2O, making phosphoenolpyruvate which contains ~ P Glycolysis 9. Transfer high-energy phosphate (~ P ) to ADP, making ATP; 2 ATPs are made PEP becomes pyruvate Glycolysis Click here to view a different animation