Download Investigation on ovine haemophilosis Mansoura, Vet.Med.J., Vol.IV

Investigation on ovine haemophilosis Mansoura, Vet.Med.J., Vol.IV, No.1 131145
Authors: Hamoda, F.K.; EL-Sawalhy, A.A.; Diab, R.A. and Mohamed, M.M
Abstract:
The present study was conducted to investigate the etiology of sudden clinical
manifestations and some mortalities in a large farm used for breeding of Arabian
horses. The most prevalent symptoms were severe colic, abdominal distention,
depression, watery diarrhea with fetid feces, severe congestion of mucous membranes
and excessive sweating. Affected animals were arched back with unpleasant odour of
respiration. Some animals exhibited laminitis and others showed hemiplegia. Few of
affected animals died within one week of onset of clinical signs. The history of the case
was associated the onset of the symptoms with the entrance of new feed lots of barley.
Samples were collected from barley and subjected for mycological examination where
some mycotoxin producing fungi e.g. Asperigllus species (A.Flavus, A.Parasitiscus &
A. Ochracious) and Fusirum species were isolated and identified. The levels of some
mycotoxins e.g. aflatoxins (B1; B2; G1; G2 & M1); Ochratoxin A; Zearalenone and T2
toxins were estimated in barley and tissues of dead animals. Immunological
investigation declared a degree of immunosuppressions in affected animals associated
with hematological changes and a significant decrease in alfa 1 and gamma globulin.
Histopathological examination revealed congestion, hemorrhage, degeneration and
necrosis with mononuclear inflammatory cellular infiltration in liver, kidney and heart.
Focal areas of malacia, perivascular hemorrhage, edema, neural degeneration and
gliosis were the main brain lesions. Bacteriological culture of fecal samples and
intestinal content of dead animals recorded salmonella, enterococci, pseudomonas,
bacillus and shigella species.
Published in: 2002
Investigation on tick-borne theileriosis in cattle with particular emphasis to effect
of immunization with somatic antigen of Boophilus annulatus ticks on
transmission and prevalence of theileriosis” Mansoura, Vet.Med.J., Vol.IV, No.1
147-164
Authors: Hamoda, F. K and Khalaf-Allah, S. S.
Abstract:
Kaluobia governorate, five of them were clinically normal and free from internal and
external parasites, while the remaining 13 lambs suffered from bloody diarrhea and
their feces had vegetative cells and oocytes of Eimeria. species. Hematological picture
indicated significant decrease of erythrocytic count and hemoglobin concentration,
while there was significant increase of PCV % and total leukocytic count. Differential
leukocytic count showed neutrophilia, lymphopenia and eosinophilia. Serum analysis
revealed significant decrease of sodium, iron, zinc, chloride, bicarbonate and total
protein levels with significant increase of potassium, total bilirubin, alanine
aminotransferase, aspartate aminotransferase, gamma-glutamyl transferase and alkaline
phosphatase activities. Also there were no changes of serum calcium, glucose and
copper levels. Treatment of coccidiosis of lambs with sulfadimethoxine was more
effective and faster than amprolium treated lambs.
Published in: 2002
Clinical, virological and pathlological investigation on buffaloes papillomatosis
In kaluobia governorate (Egypt). Zag.Vet.J.Vol. 30, No. 363-73
Authors: Hamoda, F.K., Shahein, M, A., Tantawy, A. A and Ahmed, M.H.
Abstract:
Hassan, H.Y.; Magda S EL-Sayed*; Selim, H.M.** and Hamoda, F.K***. Dept.Of
Medicine.Infectious diseases, Fac.Vet.Med.Minufiya and Alexandria* Universities
Dept.Of Animal Medicine. Fac.Vet.Med.Zag University ** and Benha Branch*** A
total of twenty-one (21) baldly sheep (1-3 years old) belonged to private flock at ELBanger area in Alexandria governorate. Fourteen of these sheep were suffering from
depraved appetite, unthriftness, rough coat and pale mucous membrane. The rest seven
sheep were clinically healthy served as control group. Sheep under investigation were
exposed to thoroughly clinical examination and parasitiological analysis including
fecal and blood examination. These animals were free from internal and external
parasitism. All animals were vaccinated with inactivated FMD vaccine. Biochemical
results showed highly significant decrease in copper, iron and ceruloplasmin. Also,
there were significant decrease of total protein, albumin and globulin. On the other
hand, the result of hemogram showed highly significant decrease in both hemoglobin
and red blood cells, while there were significant decrease in the total leukocytic count.
The differential leukocytic count showed significant decrease in the lymphocyte and
neutrophile. All these altered parameters retained nearly to normal within four weeks
postreatment. The serological analysis showed highly significant decrease in the serum
antibodies titer before and four weeks of copper treatment. On conclusion, trace
elements status especially copper plays an important role in the immune response of
vaccinated animals. So that repair copper deficiency before vaccination essential to
ensure immunity against infectious diseases.
Published in: 2002
Clinical and laboratory studies on rift valley fever vaccines of sheep.
.
Abstract:
This study was conducted on 15 balady sheep of 1-3 year age to throw light on the
effect of parasitism (mixed infestation with fascioliasis and nematodiasis) on the
immune response of sheep to PPR living attenuated cell culture vaccine. Leukogram of
infested vaccinated sheep declared significant reduction in the total leukocytes and
lymphocytes count and significant elevation in neutrophils and eosinophils. Serum
biochemical panel of infested vaccinated sheep revealed significant decrease in serum
zinc, total protein, albumin, total globulin, gamma globulin, IgG, vitamins E and C and
highly significant decrease in iron, copper, vitamins A and Beta-carotene in
comparison to the values of these statements in control vaccinated non infested sheep.
Immune response of the vaccinated animals as concluded from serum neutralization
test revealed that, the infested group were responded poorly to PPR vaccine where
serum neutralization antibodies titer were 2, 4 and 4 at 2WPV, 2MPV and 3MPV,
respectively, while the titer in control non infested sheep was 16, 64 and 64,
respectively during the same periods. So consideration is put before vaccination to
investigate the vaccinated animal clinically and coprologically and the infested animals
should be treated by efficient broad spectrum anthelmentics beside good management
and supplementation of these animals before vaccination with such elements that have
a role in the immunity and immune response to restore deficiency associated with
parasitism and correction of their level to normality to obtain maximum protective
immune response at the required optimum level and improve the outcome of
vaccination programs
Published in: 1997
Immunosuppressive effect of some water pollutants on sheep vaccinated with
bivalent lamb dysentery and pulby kidney vaccines Abou-salem, M.E.,Hamoda,
F.K., El-sayed, A.I., and Fathia Shafie,S.A. 7th Scientific Congress Fac. of Vet.
Med. Assiut University , Egypt .(1996)
Authors: El-Sawalhy, A..A., Hamoda, F.K., Khairat Abdel Megid.Elian. and Gehan, M.Kamal
Abstract:
Field outbreak of acute upper respiratory disease problem was highly spread among
Egyptian equine population at Kaluobia and Sharkia governorates from December
1999 to April 2000 in 108 (51 donkeys, 41 horses and 16 mules) of 205 (96 donkeys,
78 horses and 31 mules) equine of different ages and sexes. Clinical examination of
diseased equine revealed typical clinical signs of influenza as fever for 1-3 days,
anorexia or decrease of appetite, depression, reluctance to move, rhinitis with
congestion of nasal mucosae, initially serous to mucopurulent nasal discharge,
listlessness, frequent severe explosive non productive (dry) cough, labored breathing or
dyspnea, dilated nostrils, conjunctivitis and lung abnormality on auscultation of the
chest. The characteristic features of equine influenza in susceptible animals (harsh
cough, high temperature and rapid spread), history and epidemiological characteristic
of the disease were sufficient to permit a tentative clinical diagnosis while definitive
diagnosis was best accomplished through viral isolation and serological investigation.
The trials of virus detection from nasal swabs of diseased equine by inoculation into
embryonated chicken embryos revealed detection of the influenza A subtype 2 virus
(A/equi/2), meanwhile virus isolation from tissue specimens of sacrificed donkeys was
failed. Serological investigation (HI) revealed significant seroconversion in paired sera
taken from diseased and convalescent equine. Some epidemiological parameters,
hematological, serum biochemical and bacteriological analysis were investigated and
discussed in the light of the pertinent literature, also clinical symptomatic treatment
and lines of control were applied. Finally it could recommended that a thorough
serological and virological surveillance and a complete epizootiological investigation
should be undertaken allover Egypt to clarify the situation of the disease and the virus
during interepizootic period and is the virus present and circulate among equine
population or other reservoir subclinically during that period in Egypt? and causes new
epidemics when the immunity is breakdown or is the importation of clinically or
subclinically infected equine is the cause of appearance of new epidemics in Egypt ?.
Trial to prepare vaccine from the circulating virus type due to anecdotal information
suggests that equine influenza outbreaks have occurred previously in Egypt at 1989
and presumably will do so in the future until there is no effective vaccines prepared
and used in vaccination of Egyptian equine.
Published in: 1996