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ETANERCEPT LEVEL ELISA M1887 Your solution for fast, reproducible and specific quantification of Etanercept concentrations in plasma and serum. Kit content • Principle of the test: Coating antibody, recombinant TNF, biotinylated antibody, streptavidin-poly-HRP conjugate, HPE-dilution buffer • Reagents for four 96-wells plates (384 tests) • Comprehensive protocol Key features • Highly specific for Etanercept • Assay range optimised to clinically relevant concentrations • Basic ELISA for easy implementation in your laboratory • Research Use Only Wells of a 96 microtiter plate are coated with an anti-TNF capture antibody. Recombinant TNF is added to each well and binds to the anti-TNF capture antibody. Why monitor for Etanercept? Etanercept is a tumour necrosis factor (TNF) inhibitor that is frequently administered to patients who suffer from diseases where inflammation is an important cause of the clinical symptoms, such as rheumatoid arthritis. Inhibition of the inflammation can increase the quality of life of a patient. Studying Etanercept drug levels in relation to Patient serum/plasma or the calibration curve is added to the well. The Etanercept present in the samples will bind to TNF. clinical response may help to determine the most suitable dose of Etanercept. This knowledge may lead to an optimal Biotinylated antibody directed to the idiotype of Etanercept is added to each well. treatment schedule for Etanercept and may result in patient adjusted treatments. Sanquin Blood Supply, division Reagents Internationally active scientists from Sanquin are involved in various global (clinical) research studies in the field of TNF inhibitors. This expertise, in combination with the longstanding experience in assay development of the division Streptavidin-HRP is added to each well, followed by a colour reaction. Reagents, resulted in a high quality Etanercept level ELISA. Sanquin Blood Supply Division Reagents Telephone +31 20 512 35 99 Fax +31 20 512 35 70 E-mail [email protected] www.sanquinreagents.com