Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Work Plan and Methodology: A)Work Plan: First Year: Literature Review, collection of Sewage water, MFC setup. Qualitative analysis of wastewater. Second Year: Standardization of MFC setup, monitoring of MFCs for power generation statistical analysis . B) Methodology: I. Collection of Materials An amount of Sewage wastewater was carefully collected using gloves and was stored in a sterile, sealed container. A liter of wastewater was also collected and stored Design and set up of MFC • Ten plastic bottles (400 ml capacity each) • Zinc electrodes (length = 14.0 cm, diameter = 0.7cm) • Carbon electrodes (length = 14.0 cm, diameter = 1.5cm) • Copper wire was inserted in electrodes to connect the circuit. • Multimeter (Univolt, DT-860, Japan.) was connected to measure the voltage. • Chemicals Used:- Phosphate Buffer :- pH- 7.7 (0.2 M)., HCL- 50% • Egg Membrane. • PVC Pipe (Ordinary used pipe):- 7 cm long, 0.5 cm diameter. • Micro-organism used: - Lactobacillus. (Used one day curd). • Sewage wastewater. II. Construction of Microbial Fuel Cell Setups An MFC consists of 4 parts; the anode chamber, the cathode chamber, a permeable membrane, and an electrical circuit. i. Framework Two plastic bottles were used as chambers of an MFC. Two holes were drilled from the sides of the two plastic bottles such that a PVC pipe can be connected rigidly. Each container was about 7 cm high and about 0.5 cm in diameter. Two holes were also made on the lids of the bottles such that a copper wire can be passed through ii Membrane Preparation of membrane was obtained using acid treatment. iii. Circuit Carbon rods and Zinc rods were taken f that served as the electrodes of the microbial fuel cell. the carbon and Zinc rods have a length of 14 cm each . The carbon rods have diameter 1.5cm and Zinc rods have diameter of 0.7cm, both rods put in a beaker filled with water and were left to set for twenty-four (24) hours. In assembling the circuit, these were the materials used: copper wires, carbon and Zinc rods, four (4) alligator clips, multimeter/multitester (Univolt, DT-860, Japan.) . The black copper wire was for the anode and the red copper wire was for the cathode. The black wire was inserted to the hole in one bottle lid and was connected to a Zinc rod while the other end is connected to multimeter using an alligator clip. The same procedure was done to the red wire. The lids were sealed with acrylic. iv. Phosphate buffer Phosphate Buffer (0.2 M):- pH- 7.7 v. Anode and Cathode The anode chamber was inoculated with 25ml one day fresh curd which reach in Lactobacillus was dissolve in 340 ml of sewage water collected from local area of Akurdi. The cathode chamber was filled with phosphate buffer (0.2 M) had pH 7.7. No mediator was used in anodic chamber, during metabolism of organic waste in anodic chamber by bacteria the acidic condition were takes places, during these condition increasing voltage output were considered as stable operation condition of MFC.