Download iBind™ Western Device— enjoy hands-free western

iBind Western Device—
enjoy hands-free western blotting
™
Get your iBind™ Starter Kit
for only $999
All of the necessary items you’ll need to
begin using the iBind Western Device:
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•iBind Western Device
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•1 box of iBind Cards (10/box)
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•iBind Solution Kit
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The iBind Western System is an automated
western processing device. It’s hands-free,
reproducible, powerless and compatible with
western protocols. The blocking, primary and
secondary antibody incubation and wash steps
occur via sequential lateral flow (SLF) technology.
SLF allows for the timely release of primary
and secondary antibodies through mechanical
pressure applied from the device onto the iBind
Card. Glass fiber embedded in the iBind Card
allows the antibody and wash solutions to flow at
a constant rate over the western membrane. An
absorbent stack attached to one end of the iBind
Card functions to pull liquid across the card and
membrane. The iBind Western System provides
automated convenience and superior sensitivity
and reproducibility compared to traditional manual
methods without the need for any external power
source.
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Simple to use:
•Allows you to load your solutions and walk away
•All antibody and washing steps are automated
for you
•Sequential lateral flow controls the release of
each antibody and wash solution
•No electricity or battery required
•Compatible with HRP- and AP-conjugated
antibodies
Time savings:
•Compresses lengthy, overnight antibody
incubation times to about 2 ½ hours
•Rapid 15 minute set-up time
Find out more at lifetechnologies.com/ibind
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Superior western performance compared to
manual blotting:
Interferon a
A. iBlind
B. Manual
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•The iBind system is designed to deliver greater
sensitivity for many monoclonal and polyclonal
antibodies as compared to manual methods—
Figure 1 compares the level of sensitivity for
the iBind system versus manual western
methods
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•Combine the iBind Western System with
Novex highly specific primary and secondary
antibodies to help achieve clean western blots
that only light up your target protein
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Creb
C. iBind
D. Manual
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•Automated processing provides better blot-toblot consistency with CVs of less than 5% vs.
manual method CVs of 13%
Figure 1. Western membranes processed on the iBindTM device show superior sensitivity as compared to western membranes
processed manually. A) A western membrane with recombinant interferon-a (left to right: 50ng-6ng purified protein load) was processed
on the iBindTM device or using standard manual western processing protocols as specified by antibody manufacturer (primary antibody:
monoclonal anti-interferon antibody; secondary antibody: HRP conjugated anti-mouse). Membranes processed with iBind detect down
to 1ng of interferon-a while 2ng is detected on manually processed blots. B) A western membrane with cell lysate expressing creb (left
to right: 30ug -1ug cell lysate load) was processed on the iBindTM device or using standard manual western processing protocols as
specified by antibody manufacturer (primary antibody: polyclonal anti-creb antibody; secondary antibody: HRP conjugated anti-rabbit).
Membranes processed with iBind detect creb in 6ug of cell lysate while 10ug of lysate are needed to detect creb on manually processed
blot. Method. For all membranes, proteins were separated using the Bolt Gel Electrophoresis System and transferred using the iBlotTM
7-minute Blotting System to PVDF membranes.
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Everything you need to improve your western performance
Product
Amount
List price
Cat. No.
iBind Western Device
1
$900
SLF1000
iBind Cards
10
$120
SLF1010
iBind Solution Kit
1 kit
$60
SLF1020
Novex AP Mouse Chemiluminescent Detection Kit
2 kit
$100
SLF1021
Novex AP Rabbit Chemiluminescent Detection Kit
3 kit
$100
SLF1022
iBind Western Starter Kit
1
$999
SLF1000S
iBind Western Device 4 Pack
4
$2,700
SLF10004PK
Novex ECL Chemiluminescent Substrate Reagent Kit
1 kit
$206
WP20005
Novex Goat anti-Rabbit (H+L) cross adsorbed HRP*
1mg
$73
A16072
Novex Goat anti-Mouse (H+L) cross adsorbed HRP*
1mg
$73
A16104
TM
TM
TM
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*Find your highly specific and sensitive primary and secondary antibodies for your western analysis at lifetechnologies.com/antibodies
Get your iBind Starter Kit now.
Go to lifetechnologies.com/ibind
™
For Research Use Only. Not for use in diagnostic procedures. © 2013 Life Technologies Corporation. All rights reserved. The trademarks
mentioned herein are the property of Life Technologies Corporation and/or its affiliate(s) or their respective owners. CO114602 0813
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