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LAB STATION #1 VIRUSES ca LAB STATION #1 BACTERIAL CELL LAB STATION #1 ANIMAL CELL Cyto plas m Ribo LAB STATION #2 FUNCTION CHOICES 1. Ability to reproduce. 2. Unable to pass on to offspring inherited traits 3. Can maintain homeostasis 4. Capable of passing on traits by heredity. 5. Dependent upon other organisms to replicate. 6. Ability to metabolize and obtain energy. 7. Incapable of undergoing biochemical reactions. 8. Unable to maintain a stable internal environment. SURFACE RECEPTORS AND MARKERS Virus surface markers are shaded and host cell receptors are NOT shaded. LAB STATION #2 VIRUSES RELY ON HOST CELLS TO REPRODUCE LAB STATION #5 HELPFUL AND HARMFUL BACTERIA LAB STATION #6 INFECTIOUS DISEASE LAB STATION #3 VACCINE BASICS Medicine has come a long way over the years. The development of the vaccine kicked off an era of illness prevention unlike anything the world had ever seen. In fact, vaccinations are largely viewed as the most successful medical advancement in the history of public health. Before vaccines were introduced, smallpox killed millions, nearly 20,000 were paralyzed by polio, and rubella (German measles) caused serious birth defects in about 20,000 newborns. It is a widely accepted principle that once a person catches a certain disease, he or she is immune to it for the rest of their life. For example, once you've had the chickenpox, it's extremely unlikely that you'll ever catch it again. This is because your body, when exposed again, will recognize the disease and fight it off. The beauty of vaccines is that they help the body develop diseasefighting abilities without making you sick. Vaccines accomplish this amazing feat by tricking the body into believing it already has the full-blown disease. Here are the steps in this process, known as the "immune response": 1. The vaccine is administered. It contains weakened or dead forms of the disease. 2. The immune system identifies these foreign substances (viruses and bacteria), also known as antigens. 3. Once antigens are identified, the immune system develops proteins that circulate in the blood. These proteins are called antibodies. They fight the infection by killing the antigens. Antibodies are made by white blood cells called lymphocytes, also known as B cells. The main purpose of B cells is to create antibodies to fight infection. 4. The body stockpiles these antibodies so they are available to fight off the disease if exposed later on. Unfortunately, antibodies are disease-specific, so previously acquired chickenpox antibodies will be useless if faced with other diseases 5. It's very important to note that when the actual disease infects a person, the antigens multiply thousands and thousands of times until a raging infection is under way. The vaccine provides just enough of these antigens for the body to recognize them and complete the immune response process, therefore protecting them from exposure to the disease in the future. HOW VACCINES WORK LAB STATION #3 Vaccine-Preventable Diseases Anthrax Cervical Cancer Diphtheria Rotavirus Polio Hepatitis A Hepatitis B Haemophilus influenzae type b Smallpox Varicella Human Papillomavirus Influenza Japanese encephalitis Tuberculosis Rabies Lyme disease Measles Meningococcal Rubella Shingles Monkey pox Mumps Pertussis Tetanus Typhoid Pneumococcal Yellow Fever Source: CDC LAB STATION #4 Bacteria: Gram Staining In 1884 Hans Christian Gram, a Danish bacteriologist, attempted to find a universal stain that would work with all bacteria. In the process, he discovered that bacteria could be divided into two different groups -- one that retained a stain (absorbs it), called "gram-positive," and one that didn't, called "gram-negative." His unique method for identifying these two groups became the first step in any bacterial identification process. Even the simple determination that a bacteria specimen is gram-positive or gram- negative can direct a doctor in diagnosis, as different bacteria cause different diseases. For example, the bacteria that causes scarlet fever is gram-positive, while that which causes typhoid or cholera is gram-negative. Many antibiotics will kill Gram-positive bacteria, but Gram-negative bacteria are often tougher to kill, resisting common antibiotics. Gram Positive = Purple Gram Negative = Red/Pink Antibiotics: An antibiotic is a compound produced by one living organism that is harmful to another living organism. Antibiotics which are harmful to bacteria can be used to treat infectious disease. Antibiotics work by destroying the cell wall found in bacterial cells. In Gram-negative bacteria the cell wall is surrounded by an extra layer made of polysaccharides, proteins, and phospholipids. This layer blocks many antibiotics from reaching the cell wall. Since antibiotics, like penicillin, work by attacking the cell wall, this outer layer makes many Gram-negative bacteria resistant to such antibiotics. The emergence of antibiotic resistance is an evolutionary process that is based on selection for organisms that have enhanced ability to survive doses of antibiotics that would previously been lethal. Antibiotics like Penicillin and Erythromycin which used to be one-time miracle cures are now less effective because bacteria have become more resistant. One cause of the increasing rate of resistant bacteria is misuse of antibiotics. A common example is the prescription and use of antibiotics to treat viral infections such as the common cold in which antibiotics are useless. Lab Station #4 GRAM POSITIVE GRAM NEGATIVE