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Master of Chemistry: Physical and Analytical Chemistry M2 “Atmospheric Environment” S4 - Research project Proposed subject: CHANGES ON POLLEN GRAINS INDUCED BY O3 AND NO2 Laboratory: PC2A Physico-Chimie des Processus de Combustion et de l’Atmosphère http://pc2a.univ-lille1.fr/ Linked to the workpackage of the Labex CaPPA : WP 2 Supervisor: Nicolas Visez Email: [email protected] DESCRIPTION Pollen grain has a brief airborne life ranging from hours to days during pollination period. Pollen grain is modified by gaseous and particulate pollution during its transport in the atmosphere. The modifications of pollen grains by atmospheric pollution induce an inhibition of the reproductive capabilities of the pollen (germination), an increase of the allergenic potential and facilitate the dispersion of allergens in the fine fraction of atmospheric aerosols. The aim of this research project is to gain more knowledge on the chemical actions of gaseous pollutants on the pollen grains. Several study in PC2A have already been conducted in this specific area of interest1–4 with various pollen grains (birch, timothy grass, pine and ragweed). Here we proposed a work on the action of NO2 and a combination of NO2/O3 on pollen in atmospheric conditions. The candidate will gain a strong training into the following laboratory equipment: aerosol flow tube, aerosol sizer (SMPS and APS), gas analyser, GC-MS and GC-FID. 1.Chassard, G. et al. Kinetic of NO2 Uptake by Phleum Pratense Pollen: Chemical and Allergenic Implications. Environmental Pollution 196, 107– 113 (2015). 2.Naas, O. et al. Chemical modification of coating of Pinus halepensis pollen by ozone exposure. Environmental Pollution 214, 816–821 (2016). 3.Sénéchal, H. et al. A Review of the Effects of Major Atmospheric Pollutants on Pollen Grains, Pollen Content and Allergenicity. The Scientific World Journal 2015, ID 940243 (2015). 4.Visez, N. et al. Wind-Induced Mechanical Rupture of Birch Pollen: Potential Implications for Allergen Dispersal. Journal of Aerosol Science 89, 77–84 (2015). Keywords: Heterogeneous Chemistry, Analytical Chemistry, Experimental work