Download MICR304 IMMUNOLOGY AND SEROLOGY, SCHEDULE SPRING

MICR 4600 Theoretical and Applied Immunology Spring 2017
Lecture Section 01 (Class # 30527): MW 12:15 – 1:30 pm, SH-C366
Laboratory Section 02 (Class # 30528): M 2:00 – 4:30 pm, ASCL 229
Laboratory Section 03 (Class # 35183): W 2:00 – 4:30 pm, ASCL 229
Instructor (Lecture & Lab)
 Name: Dr. Edith Porter
 Class office hours: M: 11:30 am – 12:00 pm; W: 5:00 – 6:00 pm (walk in or by appointment); inquiries via email: any time
 Academic advisement: by appointment through the Department Office (323-343-2050), Mondays 8:45 – 9:45 am
 Office location: ASCL 355
 Contact information: [email protected] (preferred); (323) 343-6353
Prerequisites:
 MICR/BIOL 3100 or MICR 2010/2020 and CHEM 2200 and CHEM 2201, each with a grade C or higher, or instructor consent.
MOODLE:
 The course will be administered through MOODLE.
 All course related materials such as lectures, assignments, and useful links will be accessible through MOODLE.
 Enrolled students have automatically access to the course web site in MOODLE through their myCSULA portal.
 Visit http://www.calstatela.edu/moodlementor for help how to use MOODLE. Contact your instructor if you still have
trouble accessing MOODLE.
Email account:
 You must have an NIS account to access MOODLE and receive course related information. You should also provide an
alternate email address to the instructor to ensure communication when the campus email system is down.
Textbooks:
 Lecture: Janeway’s Immunobiology; 9th Edition by Murphy; Publisher: Taylor; ISBN: 9780815345053. While the 9th edition is
preferred you can also work with the 8th edition. As alternative to the University Bookstore, Cheggs, Amazon, and
Knetbooks offer affordable rent solutions.
 Laboratory: Laboratory manual by Dr. Edith Porter will be uploaded on MOODLE. Print (no need for color printing) and
place the pages in a dedicated binder. The manual will serve as your laboratory notebook. Specific guidelines will be posted.
Student learning outcomes: Upon successful completion of this course you will:
 be able to define the key players of the immune response and explain how they are orchestrated
 be able to apply this knowledge in understanding diseases related to a dysfunctional immune system
 have been introduced to fundamental techniques used in clinical immunology
 appreciate how immunology can be used as a tool in research settings
 have deepened familiarity with a biomedical laboratory environment
 have gained enhanced skills in scientific data recording, analysis, interpretation, and dissemination
 have improved your ability to formulate questions
Attendance and overall performance:
 Lecture and laboratory attendance is required. There are no make-up lab sessions. Recurrent late arrival time at the lab
sessions and not observing lab safety and housekeeping rules will lead to point deductions. Course credit will not be given if
more than 2 laboratory sessions have been missed except under extraordinary circumstances that are properly documented.
 Some experiments may run longer than the allotted class time and for some experiments you have to come in the following
day to read results.
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Performance evaluation:
Lecture: 600 points
20
30
80
40
150
100
180
1000 points total
Pre- and post-course reflective essays (2 with 10 points each)
Active learning
Case studies (team work; 3 with 10 points each, 1 final case study with 50 points)
Seminar synopsis and research question
Weekly post-lecture quizzes (15 with 10 points each)
Midterm
Final comprehensive examination
You will write two reflective essays, one at the very beginning of the class, and one at the end of the class. Instructions for these
essays will be posted on MOODLE. These essays aim to help you connect with the class and shall establish early on significance of
the class content. Active learning exercises will consist of various group and individual activities and will be incorporated in
lecture. The points assigned are for participation. Case studies will help you to apply your theoretical knowledge to clinical
settings. The cases will be assigned to teams. Detailed instructions for the case studies including resources will be posted on
MOODLE. Asking questions is the beginning of research and advancement of knowledge happens trough finding answers to these
questions. This course aims to improve your ability to ask research questions and draws from “Santana, L., & Rothstein, D. (2011).
Make just one change: Teach students to ask their own questions. Harvard Education Press: Cambridge, MA”. We will first learn
how to ask questions in general and then apply the newly gained skill in conjunction with a research seminar given by our Guest
speaker Dr. Charles L. Bevins. A seminar synopsis will be prepared based on the seminar presented by Dr. Bevins. You will
summarize the presentation, critically evaluate the talk and its content, and briefly discuss how this relates to our class, and then
present your own unique research questions ignited by the seminar. To help you process the lecture material in a timely fashion,
there will be weekly MOODLE quizzes covering the lectures from the preceding week. The quizzes will open Thursday evenings,
allow for two attempts (higher score counts), and will close Sunday nights at 23:55 (11:55 pm). Midterm and final examination
will include 10 points for a brief report on a current news article (published during the term of this quarter) in the general news
(newspapers, web etc.) that relates to immunology, either in health and disease or as a tool for diagnostic or research. An article
from a science journal is not appropriate. The typed news report will be in the following format: indicate title and author(s), the
source and date of publication; a summary of the article in your own words (~ ½ page), a short description of what captured your
attention/why you chose this article, and a brief discussion how the selected article relates to immunology and this class
specifically. You will turn in the completed report along with a hard copy of the news article during the lecture exam. Midterm
and final lecture exams will consist of multiple choice questions, short answers, some drawings (e.g. structure of an antibody
molecule) and fill-ins. The final lecture exam will consist of two components: First, individual completion of the exam during the
first 120 minutes, then completion of a portion of the exam in small groups of students during remaining time. The instructor will
assign the members of the group. The points achieved in the group activity will be added to the individual scores of each group
member as extra credit but no more than 10% of the individual score (e.g. individual score = 160 pts; small group exam 17 pts –
of these only 16 points will be added; a student of the same group who had scored individually only 130 pts would receive 13 pts
only).
Laboratory: 400 points
60 Weekly MOODLE prelab quizzes (12 with 5 points each)
70 Face-to-face quiz 1 and 2 (35 pts each)
40 4 Graphs (10 pts each, due 1 week after completion and class discussion of the experiment)
50 Poster session (Poster 30, Poster presentation 10, Poster evaluations 10)
50 Notebook
130 Final comprehensive examination
We will handle human and other animal blood products and body fluids. Thus, an individual general release form must be signed
in order to participate in all laboratory exercise. To ensure proper preparation for the lab exercises weekly pre-lab quizzes will
be administered through MOODLE. These are single attempt, multiple choice quizzes based on the laboratory manual. They will
be posted Thursday evenings and close Mondays at 11 am. Face to face quizzes and the final exam will include but are not
limited to questions with short answer prompts, fill-in tables, data analysis, and interpretation of graphs. Detailed instructions
for graphs, poster, and notebook will be given in class. Additional information on the notebook and poster session will be
posted.
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Grades:
B+:  86 % (860 pts)
B :  82 % (820 pts)
B- :  79 % (790 pts)
D+:  66 % (660 pts)
D :  63 % (630 pts)
D- :  60 % (600 pts)
F : < 60 % (< 600 pts)
In borderline cases (grade levels) participation in lecture and laboratory performance will be considered for the final grade.
A :  92 % (920 pts)
A- :  89 % (890 pts)
C+:  76 % (760 pts)
C :  72 % (720 pts)
C- :  69 % (690 pts)
Study suggestions:
 Always read the assigned chapter and study in particular the accompanying illustrations before attending lectures.
 You must have carefully read the laboratory manual for the experiment of the day before coming to lab. Weekly pre-lab
quizzes will test your preparedness.
 Form study groups with your peers and discuss what is happening in class.
 Contact your instructors by email and/or visit office hour when you are unclear about the material covered.
 Utilize the resources at the writing center (http://www.calstatela.edu/centers/write_cn/).
 Learn to recognize when you need help, and get help in a timely manner.
Keep track of your points:
Lecture
Lab
Assignment
Reflective essay 1
MOODLE post-lecture quizzes
Case study 1
Case study 2
Case study 3
Final case study
Seminar synopsis & research questions
Midterm
Final comprehensive examination
Active learning
Reflective essay 2
MOODLE pre-lab quizzes
Quiz 1
Quiz 2
Graph 1
Graph 2
Graph 3
Graph 4
Poster session
Notebook
Final comprehensive examination
Extra credit
Minus points
TOTAL
Max Pts
10
150
10
10
10
50
40
100
180
30
10
70
30
30
10
10
10
10
50
50
130
Your Pts
1000
General Policies:
No make-up examination/test/quiz/reports. Missed events will be set as “0 points” unless satisfactorily justified (e.g. doctors slip).
Assignments turned in late will not be accepted! No make-up laboratory sessions. Lecture and Laboratory absences need to be
satisfactorily justified (e.g. doctor’s appointment), and you are responsible to acquire the missed material. You must provide your
own laboratory coat, safety glasses, grease pen, pencil and colored markers. Please inform the instructor immediately about any
allergies against gloves. The Drop/Incomplete and Academic/Honesty policies explained in the University General Catalogue will
be strictly followed. Students are expected to read and abide by the University’s Academic Honesty Policy, which can be found
at http://www.calstatela.edu/academicsenate/handbook/ch5a. Students who violate this policy will be subject to disciplinary
action, and may receive a failing grade in the course for a single violation. You are responsible for the prerequisites for this course
and are encouraged to discuss any questions regarding the policies and prerequisites with the instructor. Students with
disabilities: Reasonable accommodation will be provided to any student who is registered with the Office of Students with
Disabilities and requests needed accommodation. Please contact the instructor ASAP to arrange appropriate accommodations!
You are strongly encouraged to work with the instructors throughout the course.
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Planned Schedule (subject to change)
Week
1
Day
M
Date
1.23.2017
W
1.25.2017
2
M
W
1.30.2017
2.01.2017
3
M
2.06.2017
4
W
M
2.08.2017
2.13.2017
W
2.15.2017
M
W
M
2.20.2017
2.22.2017
2.27.2017
W
M
W
M
W
M
W
3.01.2017
3.06.2017
3.08.2017
3.13.2017
3.15.2017
3.20.2017
3.22.2017
M
W
M
W
M
3.27.2017
3.29.2017
4.03.2017
4.05.2017
4.10.2017
W
4.12.2017
12
M
W
4.17.2017
4.19.2017
13
M
W
4.24.2017
4.26.2017
14
M
W
M
W
5.01.2017
5.03.2017
5.08.2017
5.10.2017
5
6
7
8
9
10
11
15
Lecture
Course requirements, student assessment
Overview
Basic concepts in Immunology (Chapter 1)
Cells, organs, and microenvironments of the
immune system
Innate Immunity
Anatomical barriers (Chapter 2)
Antimicrobial peptides & lipids (Chapter 2)
Reflective essay 1 due (10 pts)
Question formulation exercise
Complement (Chapter 2)
Case 1 (10 points)
Epithelial cells (Chapter 2)
Pattern recognition receptors (Chapter 2)
Phagocytes, Chemotaxis (Chapter 2)
NK cells (Chapter 3)
Induced responses of innate immunity
(Chapter 3)
Adaptive Immunity
Lymphocytes, lymphocyte receptor signaling
(Chapter 7)
Antibodies (Chapter 4)
Antibodies (Chapter 5)
B cells (Chapter 8)
Case 2 (10 pts)
T-cell receptor (Chapter 4, 5)
T cells (Chapter 8)
Midterm (100 pts)
MHC complex (Chapter 6)
T cell mediated immunity (Chapter 9)
The humoral immune response (Chapter 10)
Integrated dynamics of the innate and
adaptive immune response (Chapter 11)
Lab Experiment
Check in, laboratory and safety rules
Microscopy (blood cells, lymphatic organs)
Cation depletion of saliva
Lysoplate (Graph 10 pts)
Radial agar diffusion with E. coli
Epithelial cells and normal microbiota
Complement total hemolytic activity (Graph 10
pts)
Discuss: complement fixation reaction
Andrew Wilson, EHS: Lab Safety Training: Blood
(PP)
Phagocytosis
Quiz 1 (30 pts)
CRP agglutination
Immune electrophoresis Edvotek# 272
Radial immunodiffusion Edvotek #273
Quantitative ELISA Edvotek #278
SDS-PAGE (saliva)
Lysozyme immunoblot – Day 1
Lysozyme immunoblot – Day 2
Blood typing
Spring break- No classes
The mucosal immune response (Chapter 12)
Guest Seminar: Dr. Charles L. Bevins
Failures of the Immune system
Immunedeficiencies (Chapter 13)
Immunedeficiencies (Chapter 13)
Detection of antinuclear auto-antibodies
Quiz 2 (30 pts)
Introduction to Flow (lecture only)
Lymphocyte typing for CD4 and CD8 (Graph 10
pts)
Hemocytometer
Mixed Leukocyte culture – Day 1 (isolate cells and
set up culture)
Hypersensitivities (Chapter 14)
Tolerance, Autoimmunity, Transplantation
(Chapter 15)
Case 3 (10 pts)
Cancer and the immune system
Mixed Leukocyte Culture – Day 2: Human IL-2
Immunodiagnostic and Immunotherapies
ELISpot (R&D EL202)
Serology
Serology
Poster session- No experiment
Vaccines (Chapter 16)
Cancer immunotherapy (Chapter 16)
Lab final- No experiment
Final Case study (50 pts) – Student
presentations
Final Examination (180 pts): Friday, May 19, 2017; 11:20 – 1:20
Reflective essay 2 due Friday May 19, 2017; 11:55 pm
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