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Ross Lab - Biophysics
Cytoskeleton: Rich Biological System of
Interest
a)
Microtubules Shape the Cell
b)
Motor Proteins Move Objects and Microtubules
c)
Microtubule regulation through modifications and
motor proteins
10 m
1. Microtubule Motors Traffic Problems
Dynein, Kinesin, Eg5, MAPs
Using Total Internal Reflection Fluorescence (TIRF)
Microscopy
Optical trapping of motors to measure force
Green = Microtubules
Blue = DNA
2. Microtubule Severing Enzymes
Katanin, Spastin, Fidgetin
Using TIRF microscopy
March of Dimes Funded
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3. Microtubule Mechanics
Stability, Flexibility, Structural Studies
NSF funded
Ross, et. al., Biophysical Journal 2008
Website: www.people.umass.edu/rossj
Email: [email protected]
Ross Lab - Biophysics
1. Biochemistry and Molecular Biology:
Protein Design, Purification, and Characterization
2. Microscopy:
Epi-fluorescence, TIRF, electron microscopy, AFM
3. Analysis:
Image analysis, MATLAB analysis, measure velocity, intensity, Fourier
modes
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BioBootCamp 2010