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1. Soybeans are rich in protein. Raw soybeans, however, may contain a trypsin inhibitor. This is a chemical which prevents the digestion of protein. The presence of this trypsin inhibitor is genetically controlled. A single gene has alleles TA, TB and TC each of which codes for a different sort of trypsin inhibitor. These alleles are codominant so plant with the genotype TATB will produce two sorts of trypsin inhibitor, inhibitor A and inhibitor B. A fourth, recessive allele, To does not code for a trypsin inhibitor. This information is summarised in the table. (a) Allele Inhibitor produced TA A TB B TC C To None The inheritance of trypsin inhibitors in soybeans is an example of multiple allele inheritance. Explain how multiple allele inheritance differs from polygenic inheritance. ................................................................................................................................... ...................................................................................................................................(1) (b) (i) Give all the possible genotypes of a plant which only contains inhibitor A. ...........................................................................................................................(1) (ii) Give all the possible genotypes of the gametes produced by a plant which contains inhibitors B and C. ...........................................................................................................................1) (c) Two soybean plants were crossed and the seeds collected and counted. The results are shown in the table. Woodford High School Inhibitors present in seed Number of seeds A and B 23 A 25 B 48 1 (i) Complete the genetic diagram to explain the results of this cross. Phenotypes of parents:.................................. ....................................... Genotypes of parents:.................................. ....................................... Genotypes of gametes ...................................................................................... Genotypes of offspring: .................................................................................... Phenotypes of offspring Inhibitor A and Inhibitor B Inhibitor A Inhibitor B (3) (ii) A chi-squared test was applied to the data collected in this investigation. Explain why statistical tests such as the Chi-squared test should be carried out on data like these. ........................................................................................................................... ........................................................................................................................... ...........................................................................................................................(2) An investigation was carried out on the insects living on bean crops. The graph shows the relationship between the biomass of primary consumers, secondary consumers and producers. 100 Primary consumers 90 80 70 60 Consumer biomass/g m –2 50 40 30 20 Secondary consumers 10 0 0 200 400 600 800 1000 Producers biomass/g m–2 Woodford High School 2 (d) Briefly describe how the data on the biomass of the producers might have been collected. ................................................................................................................................... ................................................................................................................................... ................................................................................................................................... ...................................................................................................................................(2) (e) (i) In this investigation, the efficiency with which the energy in sunlight was converted into energy in the biomass of the producers was found to be 4%. For every 10 000 kJ of light energy falling on the beans, 400 kJ was therefore converted into energy in producer biomass. Use the data in the graph to complete the diagram below which shows the amount of biomass in the primary consumers and the secondary consumers. Energy in sunlight 10 000 kJ 4% Energy in producer biomass 400 kJ ......... % Energy in primary consumer biomass. ...... kJ ......... % Energy in secondary consumer biomass. ...... kJ (2) (ii) Explain why not all of the energy in producer biomass can be converted into energy in primary consumer biomass. ........................................................................................................................... ........................................................................................................................... ...........................................................................................................................(2) (Total 14 marks) 2. Answers should be written in continuous prose. Credit will be given for biological accuracy; the organisation and presentation of the information and the way in which the answer is expressed. Read the following passage. Photosynthesis takes place in the chloroplasts. These are disc-shaped organelles surrounded by an outer envelope consisting of two layers of membrane. Inside, there are further membranes which are arranged in stacks called grana. Surrounding these is the stroma. Chlorophyll and other lightcapturing pigments are found on the membranes of the grana and it is here that the light-dependent reaction takes place. This generates the ATP and reduced NADP which are used in the light-independent reaction in the stroma. Woodford High School 3 (a) (i) Describe the way in which ATP and reduced NADP are produced in the light-dependent reaction of photosynthesis. ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ...........................................................................................................................(5) (ii) Explain how ATP and reduced NADP are used in the light-independent reaction of photosynthesis. ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ...........................................................................................................................(4) (b) Using the information in the passage, describe how the structure of a chloroplast is adapted to its function in photosynthesis. ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... .....................................................................................................................................(3) (Total 12 marks) Woodford High School 4 3. Hares are small mammals similar to rabbits. The diagram shows how some of the hares found in southern Africa are classified. Animalia Chordata Mammalia Lagomorpha Leporidae Lepus Pronolagus Pronolagus randensis Jameson's hare (a) (i) Pronolagus rupestris Smith's hare Pronolagus crassicaudatus Natal rock hare Lepus capensis Cape hare Lepus saxatilis Scrub hare Name the genus to which the scrub hare belongs. ...........................................................................................................................(1) (ii) Name the order to which the Natal rock hare belongs. ...........................................................................................................................(1) (b) The map shows the distribution of three of these species of hare. Key Jameson's hare Smith's hare Natal rock hare Woodford High School 5 What is the evidence from the map that suggests that Smith’s hare and the Natal rock hare are different species? (i) ........................................................................................................................... ...........................................................................................................................(1) Suggest what has caused the gene pools of the two populations of Jameson’s hare to differ. (ii) ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ...........................................................................................................................(2) (Total 5 marks) 4. Moose are large herbivorous animals. (a) In a study of one population of moose, 72 animals were trapped and marked with ear tags. They were then released. One month later, fieldworkers examined 120 moose and found that 14 of these had ear tags. Use these figures to calculate the size of the moose population. Show your working. Answer:............................ (2) (b) Isle Royale is a large island in Lake Superior. Moose first colonised this island in 1900. At the time they had no predators on the island. Wolves, which are predators of moose, were introduced to the island in 1950. The graph shows the moose population from 1900. Island colonised by moose Wolves introduced 3000 2000 Moose population 1000 0 1900 Woodford High School 1910 1920 1930 1940 Date 1950 1960 1970 6 (i) Suggest an explanation for the changes in the moose population before the introduction of the wolves. ........................................................................................................................... ........................................................................................................................... ...........................................................................................................................(2) (ii) Describe the effect of introducing wolves on the moose population. ........................................................................................................................... ...........................................................................................................................(1) (Total 5 marks) 5. Answers should be written in continuous prose. Credit will be given for biological accuracy, the organisation and presentation of the information and the way in which the answer is expressed. The diagram shows part of a system designed to meet all the basic needs of a family of four living in the tropics. Crop plants Crop waste Solid waste used as fertiliser Urine and faeces Fermenter containing bacteria Liquid waste Carbon dioxide Cattle and chickens Algae in shallow pond Fish Gas used as fuel for cooking and lighting Urine and faeces Humans (a) The processes which naturally form part of the nitrogen cycle can make nitrogen contained in urine and faeces available to crop plants. Describe how these processes occur. ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... Woodford High School 7 ..................................................................................................................................... .....................................................................................................................................(6) (b) Explain the advantage in this system of: (i) growing leguminous plants such as groundnuts or beans; ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... (4) (ii) stocking the pond with fish that feed on algae rather than with carnivorous fish. ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ...........................................................................................................................(3) (Total 13 marks) 6. The island of Surtsey was formed in 1963 by a series of volcanic eruptions. Biologists have studied the colonisation of this new island by plants and animals. (a) The graph shows the number of species of plants found on the island each year for the first seven years of its existence. 16 14 12 10 Number of species 8 6 4 2 0 1963 Woodford High School 1964 1965 1966 1967 Year 1968 1969 1970 8 The development of the plant community on Surtsey illustrates the process of succession. Explain why: (i) very few species could grow on the island in the first few years after it was formed; ........................................................................................................................... ........................................................................................................................... ...........................................................................................................................(1) (ii) once some plants were growing, the rate at which new species could establish themselves increased rapidly. ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ...........................................................................................................................(2) (b) Animals also colonised Surtsey. One conclusion drawn from studying them was that particular species of animals found here had broader ecological niches than they did on the nearby mainland. Suggest why. ..................................................................................................................................... ..................................................................................................................................... .....................................................................................................................................(1) (Total 4 marks) 7. (a) The diagram shows an outline of the nitrogen cycle. Nitrogen gas in atmosphere Plant protein Animal protein Death and decay in soil Ammonia Nitrogen gas Process X Substance A Woodford High School Process Z 9 Name: (i) substance A; ........................................................................................................................... (ii) process X; ........................................................................................................................... (iii) process Z. ...........................................................................................................................(3) (b) The table shows the yield of a crop when different levels of nitrogen fertiliser were applied. (i) Nitrogen fertiliser applied/ kg hectare–1 Yield/ tonnes hectare–1 0 2.8 50 3.3 100 4.2 150 4.7 200 4.7 Describe the effect that the fertiliser had on the yield of the crop. ........................................................................................................................... ...........................................................................................................................(1) (ii) Explain two disadvantages of applying nitrogen fertiliser at levels above 150 kg hectare–1. 1......................................................................................................................... ........................................................................................................................... 2......................................................................................................................... ........................................................................................................................... (4) Woodford High School 10 (c) The graphs show the range of heights of samples of two varieties of wheat grown under the same conditions. Variety A is a modem form and Variety B is a form that was commonly grown 100 years ago. 200 Number of plants 200 150 Number of plants 100 50 150 100 50 0 0 50 100 Height / cm 150 Variety A (i) 50 100 Height / cm 150 Variety B Name the type of variation shown in these two graphs. ...........................................................................................................................(1) (ii) Describe two differences shown in the graphs between Variety A and B. 1......................................................................................................................... ........................................................................................................................... 2......................................................................................................................... ........................................................................................................................... (2) (iii) Suggest how the differences between the two varieties could have been brought about. ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... (2) (iv) Suggest the advantages that these differences could have for modern farmers. ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... (2) Assume that two genes affect the height of wheat plants and both of these genes have two alleles. The table gives a summary of some possible genotypes and phenotypes. Woodford High School 11 Genotype Phenotype height of wheat/cm AABB 100 AaBb 70 AAbb 70 aabb 40 A wheat plant that was 100 cm tall was crossed with a wheat plant that was 40 cm tall, fertilisation occurred and the seeds were planted and grown. (d) Using only the information in the table, answer the following questions. (i) What are the genotypes of the gametes in this cross? ...........................................................................................................................(1) (ii) What would be the expected genotype and phenotype of the resulting offspring? Genotype ........................................................................................................... Phenotype ..........................................................................................................(2) (Total 18 marks) Q1(a) Multiple alleles only involve one gene/one locus/polygene more than one gene: Woodford High School 1 12 (b) (c) A A A O (i) T T and T T ; (ii) T and T ; (i) Phenotypes of parents (ii) B 1 C 1 (Inhibitors) A and B (Inhibitor) B; A B Genotypes of parents T T Genotypes of offspring T T A B B O T T ; A O T T B O B B T T T T : 3 Assess probability; of results being due to chance; or 2 Significant difference; Between O & E/from Null hypothesis; (d) Random sampling/known area; Weighing all plant material (finding dry mass of plants); (e) (i) Percentage in each case is 9 – 10, 7.5 – 12.5; Energy in each case is 36 – 40kJ 3 – 5kJ; or 0.36 0.4% 36 (ii) 2 40kJ 2 0.027 0.05% 2.5 5kJ Any two from Lost as heat; in respiration; movement; excreted material: egested/not all digested: not all eaten; 2 [14] Q2. (a) (i) In context of ATP formation light raises energy level of / excites electrons; pass through carriers; energy released; ATP formed from ADP + P; In context of producing reduced NADP + protons / H ions; from photolysis / water; electrons; (ii) (b) max 5 GP converted to triose phosphate / GALP; this involves reduction; reduced NADP provides reducing power / hydrogens; ATP supplies energy for this reaction; phosphate from ATP; for production of RuBP; max 4 Membranes / (disc) shape provides large surface for light absorption; layering of membrane allows a lot of pigment; (permeable) membrane allows diffusion of gases / carbon dioxide; membranes provide surface for attachment of electron / hydrogen acceptors; stroma / matrix containing enzymes for Calvin cycle / light–independent reactions; max 3 [12] Q3. (a) (i) (b) Lepus; 1 (ii) Lagomorpha; 1 (i) Distribution / habitat overlaps / found in the same place; 1 (ii) Geographical isolation / separation / barrier; Adapted to slightly different conditions / environment / different selection pressure; Mutation in one population / different mutations; max 2 [5] Q4. (a) Allow two marks for 617; Woodford High School 13 allow one mark if arithmetical error in calculation; (b) (i) (ii) 2 Moose numbers rose in absence of predators / with plenty of food; depletion of food / spread of disease / climatic change / resulted in fall / death; 2 Caused the moose numbers to stabilise at lower level / equivalent; 1 [5] Q5. (a) Organic compounds of nitrogen / named example; converted to ammonium compounds / ammonia; by saprophytes / saprobionts / decomposers / equivalent; to nitrites; to nitrates; by nitrifying bacteria / named bacteria; uptake by roots; 6 (b) (i) (ii) max Nitrogen fixing bacteria / named e.g.; in root nodules (of legumes); convert nitrogen to ammonium / organic compounds (in legume); released on decomposition; and converted to nitrates; less need for fertiliser; max 4 Shorter food chain / less trophic levels; will produce a greater yield of fish; less energy lost between stages / more energy from producers; (energy loss) in respiration / as heat; fish prevent algal blooms / equivalent; max 3 [13] Q6. (a) (i) (Few species adapted to) hostile/harsh environment/colonising bare/ volcanic/fresh rock/little/no soil/water/humus; (ii) (b) Colonisers erode rock/release minerals; add (more) humus/increase nitrate content/organic material; when colonisers die/decompose; More/less competition; 1 max. 2 1 [4] Q7. (a) (i) (b) (c) – 1 (ii) Nitrification; 1 (iii) Denitrification; 1 –1 (i) (Increase) fertiliser increases yield up to 150 kg hectare ; (ii) (Excess) runs off/leaching/washed out; eutrophication; no extra yield; cost (only once)/economic waste; kills crops; due to osmotic effects/pH imbalance; 1 max. 4 (i) Continuous; (ii) A has a smaller range of heights; A is shorter/explained; A is skewed; (allow converse) max. 2 Mutation; selection; genetic modification; max. 2 Less stem damage in wind; more energy used for seed formation/greater yield of seed/grain; easier to harvest; max. 2 (iii) (iv) (d) – Nitrate/NO 3 /nitrite/ NO 2 ; 1 (i) AB and ab; spaced or ringed 1 (ii) Genotype: AaBb; Phenotype: 70cm 2 Woodford High School 14