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LC/MS/MS SOLUTIONS FOR CLINICAL RESEARCH The highest level of confidence LC/MS/MS SOLUTIONS FOR CLINICAL RESEARCH For Research Use Only. Not for use in diagnostic procedures. The limitations of conventional analytical techniques are accelerating the adoption of mass spectrometry as a smart alternative for scores of clinical research applications that involve trace level quantitation of multiple compounds such as endocrine analytes, biomarkers, and drugs of abuse. AB SCIEX delivers easy-to-use solutions that speed the adoption of LC/MS/MS by clinical researchers. Combining best-in-class technologies, advanced software, and pre-configured analytical methods, AB SCIEX enables clinical research laboratories to benefit from the power of LC/MS/MS technology. Faster ROI Easy-to-use methods Significant savings through lower running costs and Clinical researchers can implement LC/MS/MS pre- increased throughput. configured methods quickly and easily – even without The right answer, the first time previous mass spectrometry experience. Superior sensitivity, low detection limits, and high Maximum instrument uptime specificity for greater confidence in your results. AB SCIEX LC/MS/MS supports you with rugged, robust Faster time to results LC/MS/MS reduces sample prep and analytical run times to give you results in minutes, not hours. instrumentation and worldwide service and support. LC/MS/MS solutions for endocrinology and biomarker research LC/MS/MS is ideally suited to identify panels of steroids, vitamin D and its metabolites, peptides or proteins, and other compounds in complex matrices such as blood, urine, saliva and whole cell lysates. Tandem mass spectrometry is becoming an essential tool for • 1,25-di-OH-vitamin D2 and D3 research applications in endocrine and biomarker analysis. Unlike conventional immunoassay methods, LC/MS/MS enables highly selective and specific analysis of multiple compounds in a single • Multi-analyte steroid panel • T3/T4 and FT3/FT4 • Homocysteine run, leading to more confidence in results and opening up new discoveries. One key advantage of LC/MS/MS analysis is its ability to analyze AB SCIEX offers an extensive portfolio of pre-configured, verified a wide variety of compounds, even across different compound methods to help you confidently implement tandem mass classes. For example, you can easily combine analysis of spectrometry in your laboratory, even if you are new to the testosterone, estrogens, and mono-OH-vitamin D into a single technology. This ever-expanding portfolio of applications method. You can also add other steroids, such as OH-progesterone, already includes: cortisol, and DHEA into an LC/MS/MS method, and quantitate a panel of steroids in a single test. • Testosterone, including low level testosterone • Estrogens: estradiol, estrone, and estriol • 25-OH-vitamin D2 and D3 Cortisol With 25-years of experience delivering highperformance systems, software, and support, 5.00e5 4.00e5 3.00e5 2.00e5 1.00e5 0.00 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 17-OH Progesterone DHEA 6.00e5 Testosterone Androstenedione 7.00e5 DHEAS Intensity, cps 8.00e5 7.5 8.0 8.5 AB SCIEX is a partner you can trust to integrate easy-to-use mass spec technology into your lab’s 25-OH Vitamin D3 1.00e6 9.00e5 Progesterone 1.10e6 9.0 day-to-day research routine. Save time, cut costs, and solve analytical issues 9.5 10.0 10.5 Time, min The chromatogram above shows a method that measures the levels of a wide variety of compounds in a single analytical run. Combining multiple analytes into a single analysis increases throughput and minimizes sample consumption when sample volume is limited. with greater confidence and better results. LC/MS/MS solutions for inborn errors of metabolism and amino acid studies Inborn Errors of Metabolism Amino Acid Analysis Move your research ahead Faster time to result AB SCIEX solutions enable you to use LC/MS/MS for classical The AB SCIEX solution for LC/MS/MS analysis of amino acids neonatal screening research and expand into other applications is faster than conventional research methods and uses labeled related to inborn errors of metabolism such as research into internal standards for more accurate and robust quantitation, lysosomal storage disorder, bile acids, very long chain fatty acids with run times of less than 18 minutes per sample. for peroxysomal disorder, and steroids involved into congenital adrenal hyperplasia. AB SCIEX provides pre-configured, verified method for research into inborn errors of metabolism, including the analysis of both nonderivatized and derivatized analytes and allowing users to analyze amino acids and acylcarnitines panels simultaneously for research on over 40 metabolic disorders. The run time is less than 2 minutes for the analysis of each dried blood spot sample. 8.0e5 C2-Carnitine 7.5e5 1.4e5 7.0e5 1.2e5 6.0e5 1.1e5 1.0e5 4.5e5 3.5e5 C5-Carnitine Carnitine C8-Carnitine C4-Carnitine 3.0e5 C10-Carnitine C3-Carnitine 2.5e5 C12-Carnitine C16-Carnitine C5DC-Carnitine C14-Carnitine C18-Carnitine 2.0e4 1.5e5 Intensity, cps Intensity, cps C6-Carnitine 5.0e5 4.0e5 Asn Ser Hyp Gly Gln EtN Asp 1.3e5 6.5e5 5.5e5 Cit Sar Thr bAla Glu His 3MHis Ala Hcit 1MHis Asa GABA 1.5e5 9.0e4 8.0e4 PSer PEtN Tau 7.0e4 6.0e4 Aad Ans Car bAib Pro Arg Hyl Abu Orn Cth Cys Lys Val Met Tyr Nva Hcy Ile Leu Nle Phe Trp 5.0e4 4.0e4 3.0e4 2.0e4 1.0e5 1.0e4 5.0e4 0.0 0.0 220 240 260 280 300 320 340 360 380 400 420 440 460 480 m/z Da Example chromatogram of derivatized acylcarnitines and their corresponding internal standards 500 1 33 2 65 3 97 4 129 5 161 6 193 7 225 8 258 Time, min Separation of 45 amino acids in less than 18 minutes 9 290 10 322 11 354 12 385 13 418 LC/MS/MS solutions for research into therapeutic drug monitoring The development of new therapeutic drug monitoring (TDM) methods is an area of intense research due to: • The need for low-dose, multi-drug regimens • The importance of assessing inter- and intra-individual metabolism variability • The necessity of determining the narrow therapeutic concentration ranges As a result, clinical research laboratories are turning to LC/MS/MS to analyze immunosuppressant, antiretroviral, anticonvulsive, and antipsychotic drugs. Get it done AB SCIEX LC/MS/MS solutions quickly identify and quantify low-dose, multi-drug regimens in different matrices to provide the accuracy you need for confidence in your results with the utmost speed and sensitivity. AB SCIEX provides a direct injection method for the analysis of AB SCIEX offers a pre-configured, verified method for the cyclosporin A, everolimus, sirolimus and tacrolimus. A method quantification of antiretroviral drugs in serum in a total run for the analysis of mycophenolic acid in plasma is also available. time of 7 minutes. Sample preparation is based upon protein precipitation, centrifugation, dilution and injection. Cyclosporin D (IS) 7.0e4 10 2 6.5e4 5.5e4 9 4 6.0e4 5.0e4 13 5.5e4 Intensity, cps 4.5e4 Intensity, cps 1.Nevirapine 2.Maraviroc 3.Raltegravir 4.Amprenavir 5.Darunavir 6.Efavirenz 7.Delviradine 8.Indinavir 9.Atazanavir 10. Nelfinavir 11. Tipranavir 12. Ritonavir 13. Lopinavir 14. Saquinavir 7.5e4 6.0e4 4.0e4 3.5e4 Tacrolimus 3.0e4 2.5e4 Everolimus 2.0e4 Sirolimus 4.5e4 3.5e4 6 3 4.0e4 8 11 12 1 2.5e4 Cyclosporin A 2.0e4 1.5e4 Ascomycin (IS) 1.0e4 5.0e4 3.0e4 1.5e4 1.0e4 5000.0 0.0 5 8.2e4 9.0e4 7.0e4 6.5e4 14 7 5000.0 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 2.0 2.1 2.2 Time, min Direct injection analysis of cyclosporin A, everolimus, sirolimus and tacrolimus 2.3 2.4 0.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 2.9 3.0 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8 3.9 4.0 4.1 4.2 4.3 4.4 Time, min Quantitation of antiretroviral drugs in serum in 7 minutes LC/MS/MS solutions for toxicology LC/MS/MS has become a very valuable tool for toxicology screening in clinical research. Compared to other techniques such as GC/MS, by using LC/MS/MS, sample preparation can be simplified and run times reduced, resulting in increased throughput and faster sample turnaround times. In addition, the sensitivity of LC/MS/MS quantitative methods is capable of detecting and quantifying drugs of abuse at significantly lower levels than GC/MS. Identify and quantify hundreds of drugs in a single analysis Unique AB SCIEX QTRAP® System technology enables shortened analysis time and gives researchers more useful information from every experiment. Combined with Cliquid® Software, iMethod™ Applications, and a 1250 drugs of a abuse compound library, you are equipped with a rapid, high-throughput solution that is easy to implement and quick to deliver results. Diazepam Temazepam Oxazepam Fentanyl 7-Aminoclonazepam Hydrocodone 6-MAM Codeine Hydromorphone Oxymorphone Morphine Intensity, cps Desalkylflurazolam Norfentanyl Time, min Nine opiates and ten benzodiazepines analyzed in a single method with a total run time of 6 minutes. Transferring analysis from GC/MS to LC/MS/MS simplifies sample preparation (without the need for time-consuming SPE and derivatization), reduces analytical run time, and lowers operating and labor costs. Proven solutions for confident results AB SCIEX provides a variety of tools to simplify implementation of LC/MS/MS. Our state-of-the-art hardware provides unparalleled sensitivity for high-quality results and rugged instrumentation for maximum uptime. Cliquid® Software combined with iMethod™ Applications can get your clinical research laboratory running commonly requested assays quickly and effortlessly. Whether you are seeking an ultrasensitive assay for quantification of a single analyte or a high-throughput, multi-component method, AB SCIEX can customize a solution to meet your needs. High performance mass spectrometry Based on AB SCIEX Triple Quad™ or QTRAP® technology, coupled with industry leading HPLC, the AB SCIEX instrument portfolio includes the most sensitive platforms on the market for challenging clinical research applications such as low-level endocrine analysis. Powerful software Complete user-friendly software packages, with simplified workflow for instrument control and large data-set analysis and reporting –including LIS/LIMS compatibility. • Cliquid® Software is an essential tool for simplifying routine analyses. The point-and-click operation and four-step workflow greatly simplify even complex LC/MS/MS analysis. • With ChemoView™ Software, you can quickly screen large sample sets for multiple components using flow injection analysis on AB SCIEX triple quadrupole LC/MS/MS systems. The intuitive Cliquid® Software point-and-click operation and four step workflow greatly simplify method customization and analysis run. This provides a powerful workflow for research into inborn errors of metabolism, metabolic studies, lipid and endocrine studies, acylcarnitine profiling, and monitoring compounds such as amino acids (AA), acylcarnitines (AC), sugars, fatty The iMethod™ Applications dramatically reduce the need acids, peptides, and proteins. for method development and enable easier and accelerated • For utmost productivity you will double your throughput using the Cliquid® MPX™-2 High Throughput System to control and perform staggered injections from two HPLC systems. Pre-configured methods A continually updated portfolio of pre-configured, lab-proven, application-specific AB SCIEX methods for a broad range of compounds, each optimized for your LC/MS/MS system. implementation of mass spectrometry in the clinical research laboratory. To learn more, visit www.absciex.com/imethods. Comprehensive training, service and support AB SCIEX offers backup solutions to ensure your laboratory’s productivity and efficiency. You invest in our technology. We invest in your success. As the world leader in mass spectrometry, AB SCIEX creates solutions that are backed by the industry’s most extensive service and support organization. With a network of service professionals, experienced compliance specialists, and over 150 PhD application scientists worldwide, we are dedicated to supporting your technical needs and helping you get the most out of your AB SCIEX systems. AB SCIEX service professionals are recognized as the most highly qualified in the industry. They are certified on our instrument platforms through a rigorous 4-step certification program, with re-certification. This award-winning program helps to ensure that you receive the most efficient, highest-quality, and most up-to-date service available for AB SCIEX products and technology. Choose from flexible service plans and a variety of services for the right level of support for your laboratory’s needs and budget. Our customer support network is available to provide expert assistance in the use and application of AB SCIEX products through a comprehensive range of services, including application support, technical service, and training. Whether you access our service and support team by phone, email, on-site visits, or through our innovative remote monitoring technology, you can be confident that the AB SCIEX organization will be there for you. For more information, visit www.absciex.com For Research Use Only. Not for use in diagnostic procedures. © 2012 AB SCIEX. The trademarks mentioned herein are the property of AB Sciex Pte. Ltd. or their respective owners. AB SCIEX™ is being used under license. 4440112-02 Headquarters 500 Old Connecticut Path | Framingham, MA 01701 USA Phone 508-383-7700 www.absciex.com International Sales For our office locations please call the division headquarters or refer to our website at www.absciex.com/offices