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LC/MS/MS SOLUTIONS FOR CLINICAL RESEARCH
The highest level
of confidence
LC/MS/MS SOLUTIONS FOR CLINICAL RESEARCH
For Research Use Only. Not for use in diagnostic procedures.
The limitations of conventional analytical techniques
are accelerating the adoption of mass spectrometry
as a smart alternative for scores of clinical research
applications that involve trace level quantitation of
multiple compounds such as endocrine analytes,
biomarkers, and drugs of abuse.
AB SCIEX delivers easy-to-use solutions that speed
the adoption of LC/MS/MS by clinical researchers.
Combining best-in-class technologies, advanced
software, and pre-configured analytical methods,
AB SCIEX enables clinical research laboratories to
benefit from the power of LC/MS/MS technology.
Faster ROI
Easy-to-use methods
Significant savings through lower running costs and
Clinical researchers can implement LC/MS/MS pre-
increased throughput.
configured methods quickly and easily – even without
The right answer, the first time
previous mass spectrometry experience.
Superior sensitivity, low detection limits, and high
Maximum instrument uptime
specificity for greater confidence in your results.
AB SCIEX LC/MS/MS supports you with rugged, robust
Faster time to results
LC/MS/MS reduces sample prep and analytical run
times to give you results in minutes, not hours.
instrumentation and worldwide service and support.
LC/MS/MS solutions for endocrinology
and biomarker research
LC/MS/MS is ideally suited to identify panels of steroids, vitamin D and its metabolites,
peptides or proteins, and other compounds in complex matrices such as blood, urine,
saliva and whole cell lysates.
Tandem mass spectrometry is becoming an essential tool for
• 1,25-di-OH-vitamin D2 and D3
research applications in endocrine and biomarker analysis. Unlike
conventional immunoassay methods, LC/MS/MS enables highly
selective and specific analysis of multiple compounds in a single
• Multi-analyte steroid panel
• T3/T4 and FT3/FT4
• Homocysteine
run, leading to more confidence in results and opening up
new discoveries.
One key advantage of LC/MS/MS analysis is its ability to analyze
AB SCIEX offers an extensive portfolio of pre-configured, verified
a wide variety of compounds, even across different compound
methods to help you confidently implement tandem mass
classes. For example, you can easily combine analysis of
spectrometry in your laboratory, even if you are new to the
testosterone, estrogens, and mono-OH-vitamin D into a single
technology. This ever-expanding portfolio of applications
method. You can also add other steroids, such as OH-progesterone,
already includes:
cortisol, and DHEA into an LC/MS/MS method, and quantitate
a panel of steroids in a single test.
• Testosterone, including low level testosterone
• Estrogens: estradiol, estrone, and estriol
• 25-OH-vitamin D2 and D3
Cortisol
With 25-years of experience delivering highperformance systems, software, and support,
5.00e5
4.00e5
3.00e5
2.00e5
1.00e5
0.00
3.5
4.0
4.5
5.0
5.5
6.0
6.5
7.0
17-OH Progesterone
DHEA
6.00e5
Testosterone
Androstenedione
7.00e5
DHEAS
Intensity, cps
8.00e5
7.5
8.0
8.5
AB SCIEX is a partner you can trust to integrate
easy-to-use mass spec technology into your lab’s
25-OH Vitamin D3
1.00e6
9.00e5
Progesterone
1.10e6
9.0
day-to-day research routine.
Save time, cut costs, and solve analytical issues
9.5
10.0
10.5
Time, min
The chromatogram above shows a method that measures the levels of a wide variety
of compounds in a single analytical run. Combining multiple analytes into a single
analysis increases throughput and minimizes sample consumption when sample
volume is limited.
with greater confidence and better results.
LC/MS/MS solutions for inborn
errors of metabolism and amino
acid studies
Inborn Errors of Metabolism
Amino Acid Analysis
Move your research ahead
Faster time to result
AB SCIEX solutions enable you to use LC/MS/MS for classical
The AB SCIEX solution for LC/MS/MS analysis of amino acids
neonatal screening research and expand into other applications
is faster than conventional research methods and uses labeled
related to inborn errors of metabolism such as research into
internal standards for more accurate and robust quantitation,
lysosomal storage disorder, bile acids, very long chain fatty acids
with run times of less than 18 minutes per sample.
for peroxysomal disorder, and steroids involved into congenital
adrenal hyperplasia.
AB SCIEX provides pre-configured, verified method for research
into inborn errors of metabolism, including the analysis of both
nonderivatized and derivatized analytes and allowing users to
analyze amino acids and acylcarnitines panels simultaneously for
research on over 40 metabolic disorders. The run time is less than
2 minutes for the analysis of each dried blood spot sample.
8.0e5
C2-Carnitine
7.5e5
1.4e5
7.0e5
1.2e5
6.0e5
1.1e5
1.0e5
4.5e5
3.5e5
C5-Carnitine
Carnitine
C8-Carnitine
C4-Carnitine
3.0e5
C10-Carnitine
C3-Carnitine
2.5e5
C12-Carnitine
C16-Carnitine
C5DC-Carnitine C14-Carnitine C18-Carnitine
2.0e4
1.5e5
Intensity, cps
Intensity, cps
C6-Carnitine
5.0e5
4.0e5
Asn
Ser
Hyp
Gly
Gln
EtN
Asp
1.3e5
6.5e5
5.5e5
Cit
Sar
Thr
bAla
Glu
His
3MHis
Ala
Hcit
1MHis
Asa
GABA
1.5e5
9.0e4
8.0e4
PSer
PEtN
Tau
7.0e4
6.0e4
Aad
Ans
Car
bAib
Pro
Arg
Hyl
Abu
Orn
Cth
Cys
Lys
Val
Met
Tyr
Nva
Hcy
Ile
Leu
Nle
Phe
Trp
5.0e4
4.0e4
3.0e4
2.0e4
1.0e5
1.0e4
5.0e4
0.0
0.0
220 240
260
280
300
320
340
360
380
400
420
440
460
480
m/z Da
Example chromatogram of derivatized acylcarnitines and their corresponding
internal standards
500
1
33
2
65
3
97
4
129
5
161
6
193
7
225
8
258
Time, min
Separation of 45 amino acids in less than 18 minutes
9
290
10
322
11
354
12
385
13
418
LC/MS/MS solutions for research
into therapeutic drug monitoring
The development of new therapeutic drug monitoring (TDM) methods is an area
of intense research due to:
• The need for low-dose, multi-drug regimens
• The importance of assessing inter- and intra-individual metabolism variability
• The necessity of determining the narrow therapeutic concentration ranges
As a result, clinical research laboratories are turning to LC/MS/MS to analyze
immunosuppressant, antiretroviral, anticonvulsive, and antipsychotic drugs.
Get it done
AB SCIEX LC/MS/MS solutions quickly identify and quantify
low-dose, multi-drug regimens in different matrices to provide
the accuracy you need for confidence in your results with the
utmost speed and sensitivity.
AB SCIEX provides a direct injection method for the analysis of
AB SCIEX offers a pre-configured, verified method for the
cyclosporin A, everolimus, sirolimus and tacrolimus. A method
quantification of antiretroviral drugs in serum in a total run
for the analysis of mycophenolic acid in plasma is also available.
time of 7 minutes. Sample preparation is based upon protein
precipitation, centrifugation, dilution and injection.
Cyclosporin D (IS)
7.0e4
10
2
6.5e4
5.5e4
9
4
6.0e4
5.0e4
13
5.5e4
Intensity, cps
4.5e4
Intensity, cps
1.Nevirapine
2.Maraviroc
3.Raltegravir
4.Amprenavir
5.Darunavir
6.Efavirenz
7.Delviradine
8.Indinavir
9.Atazanavir
10. Nelfinavir
11. Tipranavir
12. Ritonavir
13. Lopinavir
14. Saquinavir
7.5e4
6.0e4
4.0e4
3.5e4
Tacrolimus
3.0e4
2.5e4
Everolimus
2.0e4
Sirolimus
4.5e4
3.5e4
6
3
4.0e4
8
11
12
1
2.5e4
Cyclosporin A
2.0e4
1.5e4
Ascomycin (IS)
1.0e4
5.0e4
3.0e4
1.5e4
1.0e4
5000.0
0.0
5
8.2e4
9.0e4
7.0e4
6.5e4
14
7
5000.0
1.0
1.1
1.2
1.3
1.4
1.5
1.6
1.7
1.8
1.9
2.0
2.1
2.2
Time, min
Direct injection analysis of cyclosporin A, everolimus, sirolimus and tacrolimus
2.3
2.4
0.0
2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 2.9 3.0 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8 3.9 4.0 4.1 4.2 4.3 4.4
Time, min
Quantitation of antiretroviral drugs in serum in 7 minutes
LC/MS/MS solutions
for toxicology
LC/MS/MS has become a very valuable tool for toxicology screening in clinical research.
Compared to other techniques such as GC/MS, by using LC/MS/MS, sample preparation can be
simplified and run times reduced, resulting in increased throughput and faster sample turnaround
times. In addition, the sensitivity of LC/MS/MS quantitative methods is capable of detecting and
quantifying drugs of abuse at significantly lower levels than GC/MS.
Identify and quantify hundreds of drugs in a single analysis
Unique AB SCIEX QTRAP® System technology enables shortened
analysis time and gives researchers more useful information from
every experiment. Combined with Cliquid® Software, iMethod™
Applications, and a 1250 drugs of a abuse compound library, you
are equipped with a rapid, high-throughput solution that is easy to
implement and quick to deliver results.
Diazepam
Temazepam
Oxazepam
Fentanyl
7-Aminoclonazepam
Hydrocodone
6-MAM
Codeine
Hydromorphone
Oxymorphone
Morphine
Intensity, cps
Desalkylflurazolam
Norfentanyl
Time, min
Nine opiates and ten benzodiazepines analyzed in a single method with a total run time of 6 minutes. Transferring analysis from GC/MS to LC/MS/MS simplifies sample preparation
(without the need for time-consuming SPE and derivatization), reduces analytical run time, and lowers operating and labor costs.
Proven solutions for confident results
AB SCIEX provides a variety of tools to simplify implementation of LC/MS/MS. Our state-of-the-art
hardware provides unparalleled sensitivity for high-quality results and rugged instrumentation for
maximum uptime. Cliquid® Software combined with iMethod™ Applications can get your clinical
research laboratory running commonly requested assays quickly and effortlessly. Whether you
are seeking an ultrasensitive assay for quantification of a single analyte or a high-throughput,
multi-component method, AB SCIEX can customize a solution to meet your needs.
High performance mass spectrometry
Based on AB SCIEX Triple Quad™ or QTRAP® technology, coupled
with industry leading HPLC, the AB SCIEX instrument portfolio
includes the most sensitive platforms on the market for challenging
clinical research applications such as low-level endocrine analysis.
Powerful software
Complete user-friendly software packages, with simplified
workflow for instrument control and large data-set analysis and
reporting –including LIS/LIMS compatibility.
• Cliquid® Software is an essential tool for simplifying routine
analyses. The point-and-click operation and four-step workflow
greatly simplify even complex LC/MS/MS analysis.
• With ChemoView™ Software, you can quickly screen large
sample sets for multiple components using flow injection
analysis on AB SCIEX triple quadrupole LC/MS/MS systems.
The intuitive Cliquid® Software point-and-click operation and four step workflow
greatly simplify method customization and analysis run.
This provides a powerful workflow for research into inborn
errors of metabolism, metabolic studies, lipid and endocrine
studies, acylcarnitine profiling, and monitoring compounds
such as amino acids (AA), acylcarnitines (AC), sugars, fatty
The iMethod™ Applications dramatically reduce the need
acids, peptides, and proteins.
for method development and enable easier and accelerated
• For utmost productivity you will double your throughput using
the Cliquid® MPX™-2 High Throughput System to control
and perform staggered injections from two HPLC systems.
Pre-configured methods
A continually updated portfolio of pre-configured, lab-proven,
application-specific AB SCIEX methods for a broad range of
compounds, each optimized for your LC/MS/MS system.
implementation of mass spectrometry in the clinical
research laboratory.
To learn more, visit www.absciex.com/imethods.
Comprehensive training, service and support
AB SCIEX offers backup solutions to ensure your laboratory’s
productivity and efficiency.
You invest in our technology.
We invest in your success.
As the world leader in mass spectrometry, AB SCIEX creates solutions that are backed by the
industry’s most extensive service and support organization. With a network of service
professionals, experienced compliance specialists, and over 150 PhD application scientists
worldwide, we are dedicated to supporting your technical needs and helping you get the
most out of your AB SCIEX systems.
AB SCIEX service professionals are recognized as the most highly qualified in the industry.
They are certified on our instrument platforms through a rigorous 4-step certification program,
with re-certification. This award-winning program helps to ensure that you receive the most
efficient, highest-quality, and most up-to-date service available for AB SCIEX products and
technology. Choose from flexible service plans and a variety of services for the right level of
support for your laboratory’s needs and budget.
Our customer support network is available to provide expert assistance in the use and application
of AB SCIEX products through a comprehensive range of services, including application support,
technical service, and training.
Whether you access our service and support team by phone, email, on-site visits, or through
our innovative remote monitoring technology, you can be confident that the AB SCIEX
organization will be there for you.
For more information, visit www.absciex.com
For Research Use Only. Not for use in diagnostic procedures.
© 2012 AB SCIEX. The trademarks mentioned herein are the property of AB Sciex Pte. Ltd. or their respective owners. AB SCIEX™ is being used under license.
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