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Please note: full preps of any of these viruses can be ordered. Inquire with me: [email protected] Please read these notes before deciding what to order: (1) “LS1L” denotes cre-dependent (2) hEF1α vectors are for long term retrograde studies. I have recently made an improvement in my packaging protocol that increases the titers of the retrograde vectors 3to-4-fold. As I shift the viruses I offer as aliquots to the new protocol , I will give them the designation “HT”. (3) For first time orders of any of the optogenetic viruses, including those containing IREScre or IRE-flpo, laboratory PIs please contact Dr. Karl Deisseroth and Charu Ramakrishnan for an MTA (4) Certain cre-and flpo-dependent AAV viruses that can be used in combination with my retrograde HSV viruses may be requested directly from Dr. Karl Deisseroth and Charu Ramakrishnan The PI of your lab should make that request, and an MTA will be necessary. Information is available at this site: http://web.stanford.edu/group/dlab/optogenetics/request_dna.html (5) This is NOT a full list of viruses available from the core. This is a list of the most commonly-used viruses, which I stock as aliquots. I may have stock aliquots that are not on the list – ask. All other viruses are available as custom preps. Inquire with me: [email protected] Regular Stock Short term vectors express at the site of injection but not in retrograde neurons Note that from here out, I will be offering stock aliquots of ST HSVs that express fluorescent proteins and cre only (see below). Any others will need to be custom orders. ST HSV- EGFP ST HSV-EYFP ST HSV-mCherry ST HSV-cre ST HSV-cre and GFP ST HSV-cre and mCherry hEF1α vectors express in retrograde neurons but not at the site of injection hEF1α-EGFP (HT) hEF1α-EYFP (HT) hEF1α-mCherry (HT) hEF1α-hChR2(H134R)-mCherry (HT) hEF1α-hChR2(H134R)-EYFP (HT) hEF1α-eNpHR3.0-EYFP (HT) hEF1α-LS1L-GFP (HT) hEF1α-LS1L-mCherry (HT) hEF1α-LS1L-EYFP (HT) hEF1α-LS1L-hChR2(H134R)-mCherry (HT) hEF1α-LS1L-hChR2(H134R)-EYFP (HT) hEF1α-LS1L-eNpR3.0-EYFP (HT) hEF1α-mCherry-IRES-cre (HT) hEF1α-EYFP-IRES-cre (HT) hEF1α-cre (HT) hEF1α-flpo (HT) hEF1α-LS1L-flpo (HT) hEF1α-mCherry-IRES-flpo (HT) hEF1α-GFP-IRES-flpo hEF1α-EYFP-IRES-flpo (HT) hEF1α-LS1L-mCherry-IRES-flpo (HT) hEF1α-LS1L-EYFP-IRES-flpo (HT) hEF1α-hM4Di-mCherry (HT) hEF1α-hM3Dq-mCherry (HT) hEF1α-LS1L-hM4Di-mCherry (HT) hEF1α-LS1L-hM3Dqi-mCherry (HT) hEF1α-LS1L-hM3Dq-IRES-citrine (HT) Cell-filling channel rhodopsins and DREADDS hEF1α-hChR2(H134R)-p2A-mCherry (HT) hEF1α-hChR2(H134R)-p2A-EYFP (HT) hEF1α-LS1L-hChR2(H134R)-p2A-mCherry (HT) hEF1α-LS1L hChR2(H134R)-p2A –EYFP (HT) hEF1α-hM4Di-IRES-citrine (HT) hEF1α-hM3Dq-IRES-citrine (HT) hEF1α-LS1L-hM4Di-IRES-citrine (HT) hEF1α-LS1L-hM3Dq-IRES-citrine (HT) hEF1α-GCaMP6f (HT) hEF1α-GCaMP6s (HT) hEF1α-LS1L-GCaMP6f (HT) hEF1α-LS1L-GCaMP6s (HT) RabiesG/TVA vectors hEF1α-rabiesG hEF1α-LS1L-rabiesG hEF1α-rabiesG co-packaged with hEF1α-TVA800 hEF1α-LS1L-rabiesG co-packaged with hEF1α-LS1L-TVA800 hEF1a-TVA950-T2A-rabiesG hEF1a-TVA66T-T2A-rabiesG hEF1a-TVA950-T2A-rabiesG-IRES-GFP hEF1a-TVA66T-T2A-rabiesG-IRES-GFP hEF1a-TVA950-T2A-rabiesG-IRES-mCherry hEF1a-TVA66T-T2A-rabiesG-IRES-mCherry hEF1a-LS1L-TVA950-T2A-rabiesG hEF1a-LS1L-TVA66T-T2A-rabiesG hEF1a-LS1L-TVA950-T2A-rabiesG-IRES-GFP hEF1a-LS1L-TVA66T-T2A-rabiesG-IRES-GFP hEF1a-LS1L-TVA950-T2A-rabiesG-IRES-mCherry hEF1a-LS1L-TVA66T-T2A-rabiesG-IRES-mCherry ALL TVA and rabies viruses will be provided at 5X titer in 20 ul aliquots. For all that coexpress GFP or mCherry, a tube of dilution buffer will be included in the shipment in case you find it necessary to dial down the expression of the fluorescent protein a bit. Presynaptic markers hEF1α-synaptophysin-mCherry (HT) hEF1α-LS1L-synaptophysin-mCherry (HT) hEF1α-synaptophysin-EYFP (HT) hEF1α-LS1L-synaptophysin-EYFP (HT) hCMV vectors are for use in zebrafish, in the peripheral nervous system, and in the spinal cord hCMV-EGFP (HT) hCMV-EYFP (HT) hCMV-mCherry (HT) hCMV-GCaMP6f hCMV-hChR2(H134R)-mCherry (HT) hCMV-hChR2(H134R)-EYFP (HT) hCMV-eNpHR3.0-EYFP (HT) hCMV-mCherry-IRES-cre (HT) hCMV-GCaMP6s (HT)