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BIRLA INSTITUTE OF TECHNOLOGY AND SCIENCE, PILANI. INSTRUCTION DIVISION COURSE HANDOUT (PART II) In addition to part I (General Handout for all courses appended to the timetable) this portion gives further specific details regarding the course. Course No. : BIO C352 Course Title : Cell & Tissue Culture Technology 1.Scope and objective of the Course: This course will provide an introduction to theory and application of tissue culture technologies. 2.Text Book: 1) Freshney, R.I., Animal cell culture : A practical approach, IRL press at Oxford university press.1992.Second Edition. 2) Naarayanswamy S; Plant Tissue Culture TMH 1994 3.Reference Books: 1) Freshney, R.I. Culture of animal cells.John Wiley & Sons, Inc., publications.1994. Third edition. 2) Koriss & Halkai, M.A.; Pharmaceutical Biotechnology: Fundamentals and Applications; Vallabh Prakashan 2000. 4. Course Plan: Lec. No. Learning Objectives Topics to be covered Reference Chap./Sec. 1. 2. 3. 4. 5. Introduction Cell culture media ----do-------do---Scaling up animal cell culture 6. 7. 8. 9. ----do-------do-------do---Cell line preservation and characterization 10. 11. ----do-------do---- 12. 13. ----do---Viable cell separation 14. Organ cell culture 15. 16. 17. ----do-------do-------do---- 18. Blood culture 19. ----do---- 20. Introduction to basic principles Preparation and sterilization Role of serum and serum free cultures Selection of appropriate media General methods and culture parameters Monolayer culture Suspension culture Immobilized culture Cell line banking ,freezing and recovery Cell line characterization Intraspecies cross-contamination, verifying tissues of origin, Immunological products Computerized cell source and database (Book) T.B.1 R.B.8 T.B.2 T.B.2 T.B.3 T.B.3 T.B.3 T.B.3 T.B.4 T.B.4 T.B.4 T.B.4 Cell separation and centrifugal elutriation, data collection and interpretation T.B.5 Organ culture techniques & Teratological studies Examples Organ culture for light microscopy Autoradiography, advantages and disadvantages of organ culture Lymphocytee culture and cell line maintanance Proliferation assay and cytotoxicity assay T.B.7 Layout of laboratory Design, layout and equipments of laboratory, laboratory safety and biohazards R.B.3,4,6 21 22 -23 Introducing plant tissue culture Plant tissue culture laboratory Chap1(TB 2) Chap2(TB 2) 24-25 26-27 How to grow plants in vitro How to proceed for in vitro culture methodologies --do-----do--- Introduction Organization(layout +requirement) In vitro culture approach & methodologies Tissue nutrition Callus culture: types and methods Suspension culture Continuous culture: Laboratory methods, Chap26(RB 2) Chap26(RB 2) 28 29-30 T.B.7 T.B.7 T.B.7 T.B.8, R.B.23 T.B.8, R.B.23 Chap3(TB 2) Chap4(TB 2) 31-33 34-35 -----do-----do---- 36-37 38 Manipulating/ Modifying plant characteristics through tissue culture -----do------ 39 40 In vitro cultural application Genetic Transformation types & choice of culture reactors Organ culture Plant micropropagation: Present state of arts Plant protoplast: Isolation, culture Prrotoplast Fusion and Applications Chap6(TB 2) Chap7(TB 2)&Class Notes Chap11(TB 2)& Class Notes ----do---- Application of plant cell culture Transgenic plants Chap27(RB 2) Class notes 5. Evaluation scheme: No. Components Duration 1 2 3 4 Test I Test II Quiz Comprehensive Examination 50 min 50 min 50 min 3 Hours Date &Time Venue Weightage (%) 20% 20% 20% 40% Nature of Component Closed Book Open Book Closed Book Closed Book 6. Chamber Consultation Hour: To be announced in the class. 7. Notices: Notices, if any concerning the course will be displayed on the notice Board of Biology Group. Instructor-in-charge BIO C352