Download 1 - BITS Pilani

BIRLA INSTITUTE OF TECHNOLOGY AND SCIENCE, PILANI.
INSTRUCTION DIVISION
COURSE HANDOUT (PART II)
In addition to part I (General Handout for all courses appended to the timetable) this
portion gives further specific details regarding the course.
Course No.
: BIO C352
Course Title
: Cell & Tissue Culture Technology
1.Scope and objective of the Course:
This course will provide an introduction to theory and application of tissue culture
technologies.
2.Text Book:
1) Freshney, R.I., Animal cell culture : A practical approach, IRL press at Oxford
university press.1992.Second Edition.
2) Naarayanswamy S; Plant Tissue Culture TMH 1994
3.Reference Books:
1) Freshney, R.I. Culture of animal cells.John Wiley & Sons, Inc., publications.1994.
Third edition.
2) Koriss & Halkai, M.A.; Pharmaceutical Biotechnology: Fundamentals and
Applications; Vallabh Prakashan 2000.
4. Course Plan:
Lec.
No.
Learning Objectives
Topics to be covered
Reference
Chap./Sec.
1.
2.
3.
4.
5.
Introduction
Cell culture media
----do-------do---Scaling up animal cell culture
6.
7.
8.
9.
----do-------do-------do---Cell line preservation and
characterization
10.
11.
----do-------do----
12.
13.
----do---Viable cell separation
14.
Organ cell culture
15.
16.
17.
----do-------do-------do----
18.
Blood culture
19.
----do----
20.
Introduction to basic principles
Preparation and sterilization
Role of serum and serum free cultures
Selection of appropriate media
General methods and culture parameters
Monolayer culture
Suspension culture
Immobilized culture
Cell line banking ,freezing and recovery
Cell line characterization
Intraspecies cross-contamination, verifying
tissues of origin, Immunological products
Computerized cell source and database
(Book)
T.B.1
R.B.8
T.B.2
T.B.2
T.B.3
T.B.3
T.B.3
T.B.3
T.B.4
T.B.4
T.B.4
T.B.4
Cell separation and centrifugal elutriation,
data collection and interpretation
T.B.5
Organ culture techniques
& Teratological studies
Examples
Organ culture for light microscopy
Autoradiography, advantages and
disadvantages of organ culture
Lymphocytee culture and cell line
maintanance
Proliferation assay and cytotoxicity assay
T.B.7
Layout of laboratory
Design, layout and equipments of
laboratory, laboratory safety and biohazards
R.B.3,4,6
21
22 -23
Introducing plant tissue culture
Plant tissue culture laboratory
Chap1(TB 2)
Chap2(TB 2)
24-25
26-27
How to grow plants in vitro
How to proceed for in vitro culture
methodologies
--do-----do---
Introduction
Organization(layout +requirement) In
vitro culture approach & methodologies
Tissue nutrition
Callus culture: types and methods
Suspension culture
Continuous culture: Laboratory methods,
Chap26(RB 2)
Chap26(RB 2)
28
29-30
T.B.7
T.B.7
T.B.7
T.B.8,
R.B.23
T.B.8,
R.B.23
Chap3(TB 2)
Chap4(TB 2)
31-33
34-35
-----do-----do----
36-37
38
Manipulating/ Modifying plant
characteristics through tissue culture
-----do------
39
40
In vitro cultural application
Genetic Transformation
types & choice of culture reactors
Organ culture
Plant micropropagation: Present state of
arts
Plant protoplast: Isolation, culture
Prrotoplast Fusion and Applications
Chap6(TB 2)
Chap7(TB
2)&Class Notes
Chap11(TB 2)&
Class Notes
----do----
Application of plant cell culture
Transgenic plants
Chap27(RB 2)
Class notes
5. Evaluation scheme:
No.
Components
Duration
1
2
3
4
Test I
Test II
Quiz
Comprehensive
Examination
50 min
50 min
50 min
3 Hours
Date &Time
Venue
Weightage
(%)
20%
20%
20%
40%
Nature of
Component
Closed Book
Open Book
Closed Book
Closed Book
6. Chamber Consultation Hour: To be announced in the class.
7. Notices: Notices, if any concerning the course will be displayed on the notice Board of
Biology Group.
Instructor-in-charge
BIO C352