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Biophysics of Cancer. From in vitro information to functional information Maria Filomena Botelho The biophysics, physics of biological functions, provides tools to address cancer at different levels ranging from understanding molecular in vitro mechanisms in vitro involved in the malignant transformation to the possibility of using these alterations for in vivo functional imaging. A longitudinal perspective of the running projects, in which a view to understanding the mechanisms that underlie the evolution of cancer is the main stream, will be presented. Through the in-depth knowledge of these mechanisms is possible to develop new methods for diagnosis and treatment of solid tumors. Nuclear medicine is a powerful tool that allows the detection with great sensitivity, through the image, location and extent of disease. Simultaneously, the functional approach which is at the core of nuclear medicine image is itself a powerful tool for studying mechanisms of disease.