Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Biacore™ systems for more information and faster decisions. Label-free interaction analysis. More answers: Deeper insights into biological realities Biological processes are “real-time” events, driven and regulated by dynamic interactions between key molecules. End-point techniques such as ELISA offer a snapshot view of interactions providing only basic information such as overall binding strength (affinity). The affinity depends on the ratio of on- and off-rates so that equal affinity interactions can have very different kinetic properties, resulting in different biological responses. Biacore systems can provide key data in real-time to discriminate these crucial differences, even for interactions where challenging targets are involved. Biacore systems are designed to help you to generate decisive, information-rich data, that will help answer key questions concerning the nature of the binding: 1.How strong? Affinity is a steady-state measurement made at equilibrium of a binding event and reflects the strength of an attraction between molecules. 2.How fast? Binding kinetics determine how fast/slow a complex forms or dissociates within a given time span and allow calculation of association and dissociation rate constants. 3.How much? Qualitative and quantitative determination of active analyte binding to a target protein. 4. How specific? Is the molecule specific for its target? Does the antibody recognize multiple derivatives? The flexibility in the Biacore assay design allows rapid assessment of cross-reactivity and specificity. 2 3 More insights: Real-time monitoring of binding events delivers deeper understanding. Biacore systems provide valuable information from high-quality molecular interaction data in a range of fields: from biological research, biotherapeutics and small-molecule drug discovery and development, through immunogenicity studies and vaccine development to quality control. The sensitivity and performance of Biacore systems enables detailed characterization of molecular interactions even for challenging targets such as membrane proteins. Biacore systems allow you to: Association: Interactants in solution bind to molecules on sensor chip surface. • Understand the relationship between molecular interaction and function. • Screen for hits and optimize leads based on selectivity, affinity, and kinetics. • Examine interactions of ions, small molecules, and multidomain proteins or viruses with targets. • Screen and characterize antibodies and proteins based on yes/no binding, affinity, and kinetics from the fastest on-rates to the slowest off-rates. • Quantitate protein by measuring the concentration of active protein with retained biological function. Biacore systems Molecules Methods Fields Antibodies Affinity Basic research Proteins Kinetics Peptides Screening Biotherapeutics discovery and development Compounds and fragments Specificity Small-molecule discovery Nucleic acids Concentration Ions Epitope binning Biotherapeutics manufacturing and quality control Virus Immunogenicity Thermodynamics Vaccine design and development Dissociation: Binders allowed to dissociate from molecule on sensor chip surface. Association Dissociation The binding (association) and dissociation of molecules to the sensor chip surface are monitored in real-time and displayed in a sensorgram. 4 5 More information: Biacore systems provide high-quality data in key applications Understanding the nature of interactions between molecules is fundamentally important for increased understanding of biological processes. Biacore surface plasmon resonance (SPR)-based sensors are widely used to study molecular interactions in real-time and are applied as powerful tools in research and drug discovery throughout to quality control and drug-release testing. Research Characterization of molecular interactions Structural/functional studies Biotherapeutics and small molecule discovery Biotherapeutics development and manufacturing Screening Critical quality attributes (CQA) Characterization Boost your protein research and enable the design of new and innovative studies. Screen and optimize your compounds based on direct label-free binding data. • Real-time monitoring of binding events gives a deeper understanding of the dynamics of molecular interactions. • Screen and select hits based on selectivity, affinity, and kinetics. • Kinetics, affinity, transition state thermodynamics, and yes/ no binding data help you prove your hypotheses and make dependable conclusions. • Optimize lead compounds through increased understanding of molecular mode of action. Lead optimization Design your vaccines and biotherapeutics with desired binding properties. • Accurate ranking and selection of antibodies and proteins based on yes/no binding, affinity, and kinetics—from very fast on-rates to very slow off-rates. Potency Reliably define CQA and monitor drug release parameters. • Fingerprint analysis of drug interaction and stability, even for new drug formats such as bispecific antibodies. • Novel opportunity for comparability and biosimilar studies. • Seamless integration into GxP-regulated workflows. Application and technical support – Training – Instrument service – Validation services 6 Release testing 7 Get more from your system. Biacore service and support. GE Healthcare Life Sciences is committed to supporting customers with innovative tools, analytics, and everyday lab reagents to uncover new insights in research or to discover, develop and manufacture drugs. More than 30 000 scientific publications and still counting... Thousands of Biacore systems are installed globally and more than 30 000 scientific articles have been published in peer-reviewed journals where Biacore systems have been featured for a wide range of applications. Experienced application specialists and service experts Our instrument service and application support is performed by specially trained service experts available close to you. They can help you to get the most out of your Biacore system for all applications and improve efficiency by minimizing your equipment system downtime. This allows you to focus on your work delivering reliable binding analysis results. To learn more about assay setup and data interpretation, GE offers a wide range of self-training tools, classroom trainings, handbooks, and lab protocols. Biacore club: Connect. Learn. Share. Biacore club is an online platform for Biacore system users that provides: • Easy access to information about your Biacore system. • Ability to explore a rich content library including videos and handbooks. • Exclusive offers on Biacore products. • Access to presentations and materials from our regional user days and DiPIA conferences. Join Biacore club to connect, share, and get access to exclusive content and offerings. 8 9 Consumables and software that deliver more GE Healthcare Life Sciences has developed a range of tools designed specifically to make Biacore assays as easy and reliable as possible, suitable for users of all levels of experience. The complete toolbox is backed by stringent development, production methods and quality control. Selected Biacore systems facilitates compliance with worldwide regulatory expectations by offering Validation Services and 21 CFR Part 11 functionality. Get started quickly at any experience level Use predefined Biacore run methods with application-relevant settings to rapidly set up your assay, or utilize the full flexibility of the control software to tailor methods to your specific needs. Reduce time to conclusive results The efficient evaluation software is designed to provide data overview, speed up data evaluation, and to shorten time to results. Quality control tools help you to assess high-quality kinetic and affinity data for improved interpretation of results. A sensor surface for every need Biacore sensor chips offer support for analysis of a wide range of interactions, backed up with a selection guide to help you select and use the right tools for the application. Save time and effort using kits and ready-made consumables Our capture kits significantly reduce assay development time and gives consistent capture of antibodies as well as the most common tags. Ready-made buffers and solutions are developed for your convenience and to ensure reliable results. 10 11 More choices for label-free interaction analysis. From research through drug discovery and development to QC. Choose the right system for your needs. Biacore 8K Biacore X100 High sensitivity and efficiency Delivering high-quality binding data to meet the toughest challenges in small-molecule/biotherapeutic screening and characterization. Reliable, built-in knowledge base Reliable insights into biological processes in multiuser environments/small-scale interaction analysis. Biacore S200 Biacore 4000 Outstanding sensitivity for cutting-edge applications Our most sensitive SPR-based sensor enables confident analysis of the smallest fragments, even against challenging targets such as GPCRs in LMW/fragment drug discovery. Convenient and productive Large-scale, label-free analysis in drug discovery providing high throughput without compromise on data quality. Biacore T200 Biacore C Reliable, versatile, and precise Versatile system for high-quality characterization of molecular interactions—from ions to viruses. Quality, compliant, and secure The world’s first SPR instrument dedicated to rapid determination of concentration in GxP environments. 12 13 GE Healthcare Bio-Sciences Corp. 100 Results Way Marlborough, MA 01752 USA For local office contact information, visit gelifesciences com/contact. gelifesciences.com/biacore GE, the GE Monogram, and Biacore are trademarks of General Electric Company. © 2016 General Electric Company. All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them. A copy of these terms and conditions is available on request. Contact your local representative for the most current information. 29244549AA 11/2016