Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download Streptococcus pyogenes Staphylococcus aureus Escherichia coli
Document related concepts
Transcript
Euroimmun/Lund University Meeting Heiko Herwald Lund University Sweden Lübeck July 7, 2016 Sepsis is a complex syndrome Streptococcus pyogenes Staphylococcus aureus • Infecting pathogen • Site of infectionFigure 2:Malmström et al a) • Disease spectrum Escherichia coli Salmonella spp. “The microorganisms ... turn out ... to be rather more like bystanders. It is our response to their presence that makes the disease. Our arsenals for fighting off bacteria are so powerful ... that we are more in danger from them than the invaders.” Lewis Thomas (1972) Host parasite interactions Streptococcus pyogenes Staphylococcus aureus Escherichia coli Salmonella spp. Pathogenesis of Severe Sepsis Infection Microbial Products (exotoxin/endotoxin) Cellular Responses Platelet Activation Coagulation Activation Oxidases Kinins Complement Coagulopathy/ Disseminated Intravascular Coagulation Vascular/Organ System Injury Multi-Organ Failure Death Cytokines TNF-, IL-1, IL-6 cell culture RT-PCR in vivo patient material sample processing mass spectroscopy BiaCore Bio-Plex IVIS electron microscopy microscopy QuantStudio™ 7 Flex Real-Time PCR System RT-PCR • Real-Time PCR System • broad range of real-time PCR-based applications through • multiplexing capabilities • interchangeable block formats. With a simplified • exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation. SPR biosensor BiaCore T200 BiaCore • • • • label-free system for detailed studies of biomolecular interactions measurements in real-time analyze interactants ranging from ions to viruses small concentrations needed Bio-Plex 200 System Bio-Plex • suspension array system • automates the reading of 96-well plates • reliable multiplex assay solution that permits analysis of up to 100 biomolecules in a single sample • yielding up to 9,600 data points in about 35 min In Vivo Imaging Instruments PerkinElmer IVIS • non-invasive real-time measurements in living animals • 2D and 3D quantitative bioluminescence and fluorescence with fast and low dose CT imaging • the system is capable of both luminescence and fluorescence modes of imaging Electron microscopy electron microscopy • high resolution • morphological studies • structural studies Microscopy microscopy • • • • tissue morphology histology immuno-histology co-localization cell culture RT-PCR in vivo patient material sample processing mass spectroscopy BiaCore Bio-Plex IVIS electron microscopy microscopy
