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The Effects of Resveratrol and
Alcohol on Short Term Memory in
Drosophila Melanogaster
By Paul Mascali
Need
Alcohol
 A drug found
through the
fermentation of
fruits
 Alcohol deactivates
the genes protein
kinase and sirtuin 1
Resveratrol
 Resveratrol is a chemical
that is found in plants
 Resveratrol activates the
protein kinase and sirituin
1
Memory Perception
Alcohols Effect on the Nerve
Alcohol effects
the Dendrites on
the ends of the
Nerve
Wernicke-Korsakoff Syndrome
results from
severe acute
deficiency of
thiamine
(Vitamin B1),
Resveratrol on lifespan in Drosophila and C.
Elegans
 Bass, 2007
 Resveratrol was inserted
in the drosophila food at
1, 3.2, 10, 32, 100, 200,
and 1000 mM
 Resveratrol was
dissolved at a 1:1000 to
molten agar NGM ratio
during preparation of
plates for C. Elegans
Drosophila Memory
Notch is required for long-term memory in Drosophila
Asaf Presente
Resveratrol effect on
Neurodegeneration
Resveratrol in prevention and
treatment of common clinical
conditions of aging
M. Andrea Markus
Alcohols Effect on
Memory
Genetic Dissociation of Ethanol Sensitivity and Memory Formation in Drosophila
Melanogaster
Holly LaFerriere 2007
A Publication of The Genetics Society of America
Purpose
The purpose of this experiment is to see
how alcohol and resveratrol effects the
short term memory in Drosophila
Melanogaster
Hypothesis
 Null Hypothesis: There will be no significant
difference between the control group, the
group exposed to alcohol, and the group
exposed to resveratrol.
 Alternative Hypothesis: The groups that are
exposed to resveratrol will show more of an
increase in short term memory than the control
and the groups exposed to alcohol
Memory Testing
Memory learning
Memory testing
Exposure to Alcohol
Air Pump
Ethanol
Beaker
Testing Chamber
Exposure to Resveratrol
Methodology
Wild Type Drosophila
Control Group
Never exposed to
Resveratrol or
ethanol
Alcohol
exposed Group
Resveratrol
exposed Group
Resveratrol and Alcohol
exposed Group
Exposed to 200 Proof
ethanol for 7 minutes
Exposed to 1000 Micro
Liters of Resveratrol
N=200
N=200
N=200
Exposed to 200 proof ethanol for
7 minutes and 1000 Micro Liters
of Resveratrol
N=200
Flies put through
shock training test
Flies tested for memory
Data put into SPSS
for a one-way
ANOVA and scheffe
post-hoc test
Budget
Vendor
Cat.#
Carolina
Np-86-1283
Page
Describe Item
811
Quantity
Unit Price
Total Price
95% Ethanol
3
N/A
Air pump
1
Already obtained
N/A
Drosophila
Exposure
Chamber
1
Already obtained
N/A
Ethanol beaker
1
Already obtained
N/A
Short term memory
monitor
1
23.5
70.5
Already obtained
4methycyclihe
xanol
N/A
Sigma-Aldrich
Online
Catalog
R5010-500MG
Resveratrol
1
1
Already obtained
$281.50
$281.50
Do-ability
Exposure to Resveratrol
Exposure to Alcohol
Bibliography
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Bibliography
Tabakoff,B; Conrell, N; &Hoffman, P.L. Alcohol tolerance. Annals of Emergency Medicine 15(9):10005-1012, 1986
Kathryn Pillips, Ethanol Tolerance in Temperate Drosophila, Journal of Experimental Biology 209, September 19, 2006
Heberlein Ulrike. Molecular Genetic Analysis of Ethanol Intoxication in Drosophila Melanogaster. Aug. 2004
Natural selection protected some east Asian Population form Alcoholism Science Daily 2003
Jama and Archives journals (2007) Almost one-third of Adults report having some Form of Alcohol use problem during their lifetime.
Science Daily
Sullivan Ashburner Hawley, Drosophila Protocols, Cold Spring Harbor Laboratory Press, 1996
Mumenthaler, Martin Gender Differences in Moderate drinking effect alcohol Health & Research World
Chein Samson Homophila: human disease gen cognates in Drosophila Nucleic Acids Research
Steven Gans Fruitflies Used to Examine Alcohol Tolerance 2000
Humenthaler Martin Gender Differences in Moderate Drinking Effects Alcohol Health & Research World
Windberg Jan Olof Drosophila Melanogaster Alcohol Dehydrogenase: Pridyct- Inhibition 1994
Ulrike Heverlein Molecular Genetic Analysis of Ethanol Intoxication in Drosohoila Melanogaster 2004 Department of Anatomy and
Program in Neuroscience
Morozava Transcriptional Response to Alcohol exposure in Drosophila Melanogaster; Genome Biology
White, Raskin H., and Kristina Jackson. "Social and Psychological Influences on Emerging Adult Drinking Behavior." 10 Jan. 2004.
Winberg, Jan-Olof, and John McKinley-McKee. "Drosophila Melanogaster Alcohol Dehydrogenase: Product-Inhibition." 1994.
Jordi Benach, Silvia, Atrian, Joan Fibla, Roser Gonzaler-Duarte ,and Rudolf Ladenstein, Structure-fuction relationships in Drosophila
Melanogaster alcohol Dehydrogenase allozymes, Copyright 2000, Center for Structural Biochemistry.
Ulrike Heberlein, Fred W. Wolf, Adrian Rothenfluh and Douglas J. Guarnier, Molecular Genetic Analysis of Ethanol Intoxication In
Drosophila Melangaster, Copyright 2004, Department of Anatomy and Program in Neuroscience.
Medina, J. (1996). The Clock of Ages. Cambridge: Cambridge University Press.
Hyung-Lyun Kang , Seymour Benzer , Kyung-Tai Min, Life extension in Drosophila by feeding a drug. . 2002 Jan