Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Drug design wikipedia , lookup
Pharmacogenomics wikipedia , lookup
Pharmacokinetics wikipedia , lookup
Pharmaceutical industry wikipedia , lookup
Prescription drug prices in the United States wikipedia , lookup
Prescription costs wikipedia , lookup
Drug discovery wikipedia , lookup
Drug interaction wikipedia , lookup
Pharmacognosy wikipedia , lookup
Neuropharmacology wikipedia , lookup
31817 Drosophila melanogaster, a Model System for High-Throughput Screening of Drug-Induced Central Nervous System Depression Brian Tavakoli Mentor: Mahtab Jafari Central nervous system (CNS) research is highly dependent on in vivo behavioral research, in which a live animal is subjected to a sequence of tests to determine the behavioral effect of a drug. Currently, behavioral testing on model animals is a very time-consuming, costly, and subjective process requiring a significant amount of behavioral research expertise. The approach to model the phenotypical effects of drugs on the central nervous system in Drosophila melanogaster offers many advantages in efficiency. Comparisons between the fly and human genomes indicate a high degree of conservation in fundamental biological pathways. Large-scale pharmacological screens of drugs are possible since flies have a complex nervous system and brain, yet minimal barrier to prevent access to central nervous system tissue. In this project, we aimed to design a method to efficiently test the effects of drugs on the central nervous system of Drosophila melanogaster. We orally administered different dosages of modanaifil (Provigil®), a memory-improving and mood-brightening psycho stimulant, and tested for any significant correlations in mating success. The experiment was carried out 121 times by competing drugged vs. non-drugged males in vying for copulation with virgin females. A dose-response analysis concluded that at low and high doses, modanafil had a statistically significant effect on mating success. The dose-response data proves that the drug was effectively uptaken by the subjects and the CNS was perturbed. These results offer great motivation in further developing a model system using Drosophila melanogaster for high-throughput testing and creating a system to cost-effectively screen compounds for the CNS.