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CALORIC RESTRICTION INFLUENCES LIFESPAN OF DROSOPHILA MELANOGASTER INDIVIDUALS WHOSE GENES CONTROLLING CIRCADIAN RHYTHMS ARE OVEREXPRESSED IN PERIPHERAL TISSUES 1 E. Dobrovolskaya1, E. Plysnina1,2, I. Solovev2, A. Moskaliev1,2,3 Institute of Biology of Komi Science Center of Ural Branch of RAS, Syktyvkar 2 Syktyvkar State University, Syktyvkar 3 Moscow Institute of Physics and Technology (State University) Russia The aging process is associated with various genes' expression level changes. Genes forming a system of "biological clock" of the body are not an exception, it evidenced by the worsening with age violations in rhythms of physiological activity and aperiodizm of sleep and wakefulness cycles phenomenon in old specimens. The molecular clock found in each cell of the peripheral tissues of multicellular organisms, are present and all unicellular, including prokaryotes (Shnol, 1996). The main environmental factor connected to the rhythms of biological processes is light, with daily and annual variations in the light intensity are associated such phenomena as sleep, physical activity, rest, growth, reproduction, sexual behavior, moult and migration. Most human genes of circadian rhythms are evolutionarily conserved and have orthologs in the fruit fly Drosophila melanogaster. It is found that adults of D. melanogaster show the decrease in gene expression of photosensitive protein Cryptochrome, while its overexpression in old specimens slows down the rate of aging (Rakshit, Jadwiga, 2013). On the other hand, fruit flies with mutations in the genes of circadian rhythms are characterized by a reduced life expectancy (Kondratov, Antoch, 2007). One of the cyc gene ortholog profed roles is way dTOR inhibition, resulting in metabolism supression, which prevents uncontrolled flow of resources and prolonging life of an organism (Giebultovicz et al., 2010). The purpose of this study was to investigate wheather caloric restriction affected the life span of Drosophila melanogaster which genes of circadian rhythms (Period, Timeless, Doubletime, Clock, Cycle, Cryptochrome) had been overexpressed. We chose UAS / GAL4 system to еnsure conditioned (mifepristone-inducible) gene overexpression in flies' muscules. Next, Drosophila lines were placed on standard media with different caloric values life expectancy had been being observed once a day. The results of this study demonstrate the relationship of circadian rhythms' gene regulation mechanisms and aging components such as insulinlike and TOR signaling pathways. This work was supported by a grant from the President of the Russian Federation № MD-1090.2014.4 "Comparison of response mechanisms Drosophila melanogaster to oxidative, heat, cold, and genotoxic stresses using genome-wide transcriptome analysis."