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BIOLOGY STUDY SHEET FOR MIDTERMS 1) CHEMISTRY Reading an Element Bohr’s Model Dehydration Synthesis Taking out water to make a complex substance Lewis Structure Hydrolysis Adding water to break apart a molecule Diatomic Molecule A molecule only two atoms. Ex) CO, H2, N2 Ions A molecule that gains electrons (-) or loses electrons (+) CHNOPS Carbon, Hydrogen, Nitrogen, Oxygen, Phosphorous, Sulfur Functional Groups of Macromolecules Biomacromolecules I. WATER Polar Covalent Bonds Partial sharing of electrons when the electron pair is closer to the more electronegative atom Intermolecular Forces Between 2 molecules of water Intramolecular Forces Interactive forces inside a molecule of water 2) TAXONOMY Taxonomy Naming organisms based on their characteristics Carolus Linnaeus Father of binomial nomenclature Binomial Nomenclature Identifying an organism by first the genus and then the species Species An organism capable of breeding offspring of its own kind 3) MICROSCOPE Parts Electronic Shows organelles Calculating Magnification Ocular Lens (10) x Objective (4, 10, 40) Magnification (40,100,400) mm into Micrometers Multiply x1000 Magnification vs. Resolution When the magnification is increased, the resolution needs to be adjusted to see the small details. Cell Theory 1. Cells are the basic structural and functional units of life. 2. If it is a living thing, then it must contain one or more cells. 3. All cells arise from pre-existing cells. Pseudopods Temporary projections of the cytoplasm used for locomotion Protista A kingdom containing eukaryotic organisms that have one or more cells and also a nucleus enclosed by a membrane. Paramecium 4) CELLS Organelles Cell wall Cell membrane Nuclear membrane Cytoplasm Endoplasmic reticulum Golgi bodies Ribosome Vacuoles Chloroplast Nucleolus Nucleus Chromosomes Mitochondria Microtubules Microfilaments Lysosomes Functions Protects and supports plant cell and maintains shape. Controls transport of materials into and out of cell Controls transport of materials into and out of the nucleus Provides an organized watery environment in which life functions take place by means of organelles contained in it. Provides channels through which transport of materials occurs in cytoplasm. Packages secretions for discharge from the cell Sites for protein synthesis Storage sacs fro water, dissolved materials, and wastes; maintain internal pressure of cell Continual chlorophyll in green plant cells; act as sites for food manufacture Contains centrioles that function during reproduction of animal cells The information center for cell reproduction and control of cell functions Contain the hereditary material; are agents for distribution f hereditary information. Cites of cellular respiration and ATP production Serves as support of the cell Aid in cell movement Have chemical used in cellular digestion 6) HIERARCHY OF LIFE Hierarchy of Life 1. Subatomic particle 2. Atom 3. Element 4. Compound 5. Micromolecule 6. Macromolecule 7. Organelles 8. Cell 9. Tissue 10. Organ 11. Organ system 12. Organism 13. Population – a group of organisms that inhabit a specific area 14. Community – a group of organism sharing an environment 15. Ecosystem – a natural unit consisting of all plants, animals, and microorganisms 16. Biosphere – a global ecosystem 4) ENZYMES Substrate Complex The attachment of the substrate + the complex Active Site To where the substrate attaches to Factors that affect the rate of action of an enzyme 1) pH Most enzymes have an optimum pH. A pH that is too high or too low can denaturizes enzymes and cause it to be ineffective. 2) Temperature If the temperature is too high, the enzyme unravels If it is too low, the chemical process slows down 3) Concentration of enzymes and substrates With a fixed amount of enzyme and excess substrate, the reaction speeds up then levels off. 5) DIFFUSION/OSMOSIS Passive Transport No energy required; simple diffusion and facilitated diffusion a) Simple Diffusion Movement of non-polar molecules from high to low concentration across the membrane b) Facilitated Diffusion Movement of polar molecules from high to low concentration using a protein channel Active Transport Movement of molecules from low to high concentration using a protein pump and ATP Osmosis Movement of water into a highly concentrated substance from a low concentration substance. 6) INDICATORS Indicator Test Benedict’s Solution (+ Heat) Lugol’s Solution Biuret’s Solution Brown Bag (Paper) Test For Simple Sugars Negative Result (Color) Blue Positive Result (Color) Orange Starches Proteins Lipids Amber Transparent/Gray Brown Black Lavender Translucent 7) PHOTOSYNTHESIS Reactants and Products Light Dependant Reaction Location: Grana Function: Produce ATP (energy carrier) and NADPH (electron carrier) for the dark reaction 1) Light goes to Photosystem II 2) Electrons from the water in the grana go to the electron transport chain (Oxygen secreted) 3) The electrons go through the ETC to Photosystem I and ATP is produced. 4) Photosystem I combines the electrons with NADP+ to form NADPH Light Independent (Dark) Reaction Location: Stroma (Liquid matrix of the chloroplast) Function: To produce glucose 1) 6CO2 (1-Carbon) bonds to 6RuBP (5-Carbon) to make 6 6-Carbon molecule 2) The 6C molecules break down because they’re unstable into 12 3C molecules called PGA 3) ATP and NADPH energize PGA and make it PGAL, so now we have 12 PGAL molecules 4) 2 PGAL bond to make glucose while 10 PGAL are recycled back into RuBP Chloroplast Anatomy Outer Membrane- Membrane that protects the organelle Intermembrane Space- Located between inner and outer membrane Inner Membrane- Innermost membrane that protects the organelles in the chloroplast Thylakoid- Flat membrane sacks that house chlorophyll Grana- Stacks of thylakoids (Location of Light Dependant Reaction) Lumen- The inside of the thylakoid Lamella- Thin film connecting granum Stroma- Liquid outside the thylakoids containing DNA, ribosomes, and products of photosynthesis (Location of the Dark Reaction) Why do leaves change color in the autumn/winter? Because there is less sunlight Chromatography Using laboratory techniques to separate mixtures Photolysis Using light to break apart molecules 8) LEAVES Parts of a Leaf Cuticle- waxy layer that prevents water loss Upper Epidermis- Flat cells that protect the inner tissues Palisade Mesophyll- Primary site of photosynthesis because it has many chloroplasts “Spongy” Mesophyll- Irregularly shaped cells allowing for the diffusion of CO2 Bundle Sheath Cells- Conduct water to the xylem, to the mesophyll, to the intercellular spaces; store water; prevent air from entering the xylem Xylem- Transports water Phloem- Transports food Stomata- Permit exchange of moisture and CO2 Guard Cells- Open and close the stomata Lower Epidermis- Protects the cell tissues 9) CELLULAR RESPIRATION Reactants and Products C6H12O6 + O2 → CO2 + Glucose Oxygen Carbon Dioxide H2O + Energy Water Anaerobic Respiration (Glycolysis) Location: Cytoplasm Products: 2 ATP; Ethanol + CO2 OR Lactic Acid Aerobic Respiration (Krebs Cycle and ETC) Krebs Cycle Location: Mitochondrial Matrix. Products: 2 ATP; 6 NADH; 4 CO2; 2 FADH2 Electron Transport System Location: Inner Mitochondrial Membrane. Products: 32-34 ATP; H2O Parts of a Mitochondria 10) BODY SYSTEMS I. Digestive System Food enters the oral cavity, where it begins physical digestion. Food is bit into smaller pieces to increase the surface area of chemical digestion. Salivary glands release saliva, which contains amylase, an enzyme that breaks down polysaccharides. Carbohydrates are broken down – for example, starch is broken down into glucose. All this travels down the esophagus, where it is propelled into the stomach when the epiglottis covers the air pathway. At the stomach, food goes through physical digestion as muscle contractions churn the food. It stores ingested food to release slowly into the small intestine. The lining produces: Hydrochloric acid: activates pepsin. Pepsin: protease enzyme, breaking down proteins to peptides. Mucus: protection prevents the stomach from self-digestion. The liver produces bile, which is stored in the gallbladder. It also stores fats and carbohydrates, regulates blood glucose levels, synthesizes blood proteins, stores vitamins, and detoxifies the body. The pancreas creates pancreatic juice, which provides an abundance of enzymes that digest food later on in the small intestine. In the small intestine, chemical breakdown and absorption of food molecules occur with the help from pancreatic juice and bile. Bile is a fat emulsifier, which breaks fat globules to increase surface area for chemical digestion. Lipase from the pancreatic juice then takes over the chemical digestion – they are broken down to glycerol and fatty acids. Peptidases –break down small peptides into amino acids through hydrolysis. Dissacharidases/ carbohydrases – breaks down dissaccharides into monosaccharides through hydrolysis. Amino acids, glycerol, fatty acids, and monosaccharides pass through the wall into the bloodstream as they are absorbed through villi. Fiber, usually in the form of cellulose, is indigestible and stimulates the release of H2O. It bulks up the waste and keeps the intestines healthy. At the large intestine, the bacteria created there produce vitamins that are absorbed along with water and salts. Elimination of undigested material occurs here, where it is led through the rectum out the anus. Breakdown Humans Mouth → Pharynx → Esophagus → Stomach → Small Intestine → Large Intestine → Rectum → Anus Worms Mouth → Pharynx → Esophagus → Crop → Gizzard → Intestine Grasshopper Mouth → Esophagus → Crop → Gizzard → Stomach → Hindgut II. Circulatory System Human Heart Blood enters through the superior vena cava (above the heart) and the inferior vena cava (below the heart). These structures that bring deoxygenated blood to the heart from the muscles are called veins. The blood enters the right atrium, and as the ventricles contract, the blood passively goes through the tricuspid valve to the right ventricle, where it is pushed up the pulmonary valve to the pulmonary arteries. They lead to the left and right lungs. When the blood circles back, they come through the pulmonary veins to the left atrium. There, it goes through the mitral valves to the left ventricle, where it is pushed up the aortic valve to the aorta. The newly oxygenated blood goes to the rest of the body through arteries. Controlling Heartbeat SinoA trial node (SA node or pacemaker): Location: the right atrium of the heart near the entrance of the superior vena cava Function: initiates and generates electrical impulses in order for the contraction to occur; controls the contractions of the atrium Atrioventricular node (AV node): Location: an area between the atria and the ventricles of the heart Function: which conducts the normal electrical impulse from the atria to the ventricles; controls the contractions of the ventricles Bundle of His: Location: between the atria and the ventricles to the point of the apex of the fascicular branches Function: transmits the electrical impulses from the AV node Breakdown Humans Superior/Inferior vena cava → Right Atrium → Tricuspid valve → Right ventricle → Pulmonic valve → Pulmonary artery → Lungs → Pulmonary veins → Left atrium → Bicuspid/Mitral valve → Left ventricle → Aortic valve → Aorta and into the body Worm Anus → Dorso-subneural vessel → Intestine → Dorsal vessel → Hearts (aortic arches) → Mouth → Ventral vessel → Subneural vessel Grasshopper Open Circulatory System Heart Attack When oxygenated blood is blocked from getting to the heart III. Respiratory System Pathway Nasal passage: exchanges carbon dioxide and oxygen Lined with mucus used to trap bacteria Hairs also remove unwanted “germs” Pharynx: (back of the throat) transports air from the mouth and nose to the lungs Larynx: (“voice box”) vocal chords vibrate with the passage of air Trachea: (“wind pipe”) transports air to the bronchi Bronchial tubes: transports air to small vessels called bronchioles Pleura: membrane fluid filled sac surrounding the lungs that keeps the lungs moist to facilitate diffusion Bronchioles: smaller vessels used to transport air to the alveoli Alveoli: allows the direct diffusion of oxygen and carbon dioxide in and out of the blood and tissue Diaphragm: expands the chest cavity to allow the maximum amount of air into the lungs Transport of Carbon Dioxide into/out of the Blood CO2 makes blood have an acidic pH In order for carbon dioxide to be removed from the tissues, it must be carried on RBC (red blood cell) Carbon dioxide must be converted to bicarbonate using carbonic amyhydrase H2O + CO2 H2CO3 H+ + HCO3water carbon dioxide carbonic acid Hydrogen bicarbonate H+ dissociates from carbonic acid to produce bicarbonate Transport of red blood cells in and out of the lungs They go through the capillaries near the arterioles Emphysema A condition in which the air sacs of the lungs are damaged and enlarged, causing breathlessness. Bronchitis Inflammation of the mucous membrane in the bronchial tubes Breakdown Humans Nasal Passage → Pharynx → Larynx → Trachea → Bronchial Tubes → Pleura → Bronchioles → Alveoli → Diaphragm Worms It has no circulatory system. It takes in oxygen through its skin and gives off CO2 Grasshopper Spiracles in the abdomen take in air and use trachea to conduct air to cells. Cells are always near one of these tubes. IV. Excretory System Excretion Removal of waste from metabolic processes Waste Removed 1) Urea- water, uric acid, salts, hormones, and products of hemoglobin Hemoglobin: pigment used to transport oxygen in the blood Nitrogenous wastes: excess amino acids due to the digestion of protein Ammonia: substance made when amino acids break down through deamination 2) CO2 3) H2O 4) Salts What Organs Make up the Excretory System? Kidneys: remove urine Lungs: remove carbon dioxide Liver: participate in deamination Sweat glands The Kidneys Secrete nitrogenous waste Regulate the concentration of salt in the body Maintain a stable internal environment for cells The Nephron (Functional Unit of the Kidney) Steps in the nephron filtration process: Bowman’s capsule: water is forced out of the glomerular capillaries into the Bowman’s capsule. This water is funneled into the tubules Glomerular capillaries: highly permeable to water. Brings waste products to the nephron Proximal tubule: highly permeable to water. Water follows the nutrients out of this tubule. Toxins remain in the tubule Descending limb: thin tube. Permeable to water and not to salt and minerals Loop of Henle: essential for urine concentration. Water leaves this portion of the neuron and what is left is a high concentration of salt Ascending limb: thicker tube. Impermeable to water and urea. Permeable to salt. Salt is pumped out leaving waste behind Distal tube: transfers filtrate to the collecting duct Collecting duct: highly permeable to water. Utilizes the hormone ADH (antidiuretic hormone) ADH present: water moves out of the collecting tube ADH absent: collecting duct is impermeable to water Flow of blood through the nephron: aorta => renal artery => afferent arteriole => glomerulus => efferent arteriole => peritubular capillaries => venules => renal vein => vena cava You drink too little water Blood volume low, high solute concentration in urine ADH secreted High reabsorption of water You drink too much water Blood volume high, dilute urine No ADH secreted Distal tubule not permeable to water Filtration Location: Bowman’s Capsule/Glomerulus Function: to pass pressurized blood over a filter (glomerulus) to form a filtrate (water, glucose, amino acids, and salts) *Note: proteins and blood cells remain in the glomerulus Selective reabsorption Location: proximal tubule Function: useful items are reabsorbed (glucose, water, and amino acids) while wastes and water remains in the tube *Note: reabsorption of water occurs through structures called microvilli via osmosis however, active transport is required for the reabsorption of glucose and amino acids Concentration and dilution Location: 1. Loop of Henle – as water is reabsorbed into the blood the filtrate becomes more concentrated 2. Distal tubule – requires the hormone ADH (antidiuretic hormone) for the reabsorption of water Ions Na+, Cl-, K+ Function: urine (dilute or concentrated) will be secreted *Note: transport of urine out of the body nephron => collecting ducts => renal pelvis => ureter => urethra => out In Earthworm Nephridia carry around wastes and dispose of them in the gut. In Grasshopper Maliphagan tools carry around the waste 11) BLOOD Blood Types Rh Factor An additional protein that is present in some blood but not in others Blood Pressure The force of blood against the walls of arteries Hypertension Abnormally high blood pressure 12) IMMUNITY Active Immunity Exposed to pathogen Passive Immunity Received from a parent Allergy When your immune system responds to a usually harmless substance in a defensive way Koch’s Postulates 1. The microorganism must be found in abundance in all organisms suffering from the disease, but should not be found in healthy animals. 2. The microorganism must be isolated from a diseased organism and grown in pure culture. 3. The cultured microorganism should cause disease when introduced into a healthy organism. 4. The microorganism must be re-isolated from the inoculated, diseased experimental host and identified as being identical to the original specific causative agent. Non-specific external barriers The skin and mucus membranes. If the pathogen penetrates and enters the body… Non-specific internal defenses These include phagocytes and natural killer cells, fever, and inflammation. Inflammation 1. Tissue is wounded and bacteria enter. 2. Wounded tissues send signals to increase white-blood cell production and stimulate local mast cells to release histamine. 3. Histamine causes the capillaries to become leaky and increase blood flow. 4. White blood cells and fluids seep out of the capillaries. White blood cells engulf the pathogen while the fluids cause swelling at the wound. 5. Local capillary clotting occurs to prevent future invasions. Specific internal defenses These include T and B lymphocytes that engage in cell-mediated and humoral immunity. Humoral Immunity 1. Each B-cell contains only one type of antibody receptor on its surface, and has a matching antigen. The antigen is identified by one of the B-cells. 2. B-cells produce memory cells, which are kept for the future so that immune responses to a certain antigen will be faster and plasma cells, which release antibodies that kill the bacteria. * B-cells are produced and matured in the bone marrow, and then sent out to lymph nodes and the circulatory system. Cell-Mediated Immunity 1. In order to be activated, T-cells must have viruses, or viral antigens, presented to them by other cells such as macrophages. 2. When activated, T-cells produce memory cells to speed up future invasions by the same virus, cytotoxic cells to search for the viruses and kill them, and helper T-cells to amplify the immune response of both B and T cells. * T-cells are produced in the bone marrow, and mature in the thymus.