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Surface area: volume ratio All cells need to take in oxygen, glucose, water and other nutrition. They need to remove waste e.g. carbon dioxide, heat. As a cell, or organism, gets bigger, it requires more substance exchange. But as it gets bigger, the surface area: volume ratio decreases. Larger organisms have developed transport mechanisms to allow substance exchange, e.g. heart and blood vessels, lungs, small intestine, xylem and phloem. CELL SIZE is limited by the limit of productive surface area to volume ratio.