Download The George Washington University School of Medicine and Health

Survey
yes no Was this document useful for you?
   Thank you for your participation!

* Your assessment is very important for improving the work of artificial intelligence, which forms the content of this project

Document related concepts

Proteomics wikipedia, lookup

Protein mass spectrometry wikipedia, lookup

Transcript
Fatah Kashanchi, Ph.D.
The George Washington University School of
Medicine and Health Sciences
•
•
•
•
Genomics and Proteomics of HIV and other related human
retroviruses
Resources
– 12 members
– NIH, DOD, Keck, Snyder funding
– Affymetrix, MALDI, LCQ
Active collaborations with GWU, NIH, TIGR
Output
– 86 peer reviewed publications
– Director of Genomics and Proteomics MS program
– Co-Director of the Institute for Proteomics Technology and
development
bcmfxk@gwumc.edu
202-994-1781
Human Retroviruses
Apoptotic
Anti-Apoptotic
(Cancerous)
HIV
(AIDS)
HTLV-1
(ATL)
Overall approach: Genomics and Proteomics
- Standard Microarrays, siRNA, ChIP-Chip
- Virus Chip with TIGR
- Proteomics and Mass Spec
Drug studies and Membrane Proteome
- New animal model for HIV using Stem cells (In
collaboration with Dr. Saifuddin (Conrad))
Complex
Microarray
(Computer assisted data
analysis)
Gene
Narrow down to ~20-50 genes to define
individual pathways.
“Guilt by Association” for unknown genes or
for ~2 fold differences
Detection of protein complexes (Use of
conventional and immunoaffinity
chromatography)
Mass Spec to determine
individual partners
Protein
Biochemical/Functional
Assays
Simple
Localization
studies