Download The George Washington University School of Medicine and Health

Fatah Kashanchi, Ph.D.
The George Washington University School of
Medicine and Health Sciences
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Genomics and Proteomics of HIV and other related human
retroviruses
Resources
– 12 members
– NIH, DOD, Keck, Snyder funding
– Affymetrix, MALDI, LCQ
Active collaborations with GWU, NIH, TIGR
Output
– 86 peer reviewed publications
– Director of Genomics and Proteomics MS program
– Co-Director of the Institute for Proteomics Technology and
development
[email protected]
202-994-1781
Human Retroviruses
Apoptotic
Anti-Apoptotic
(Cancerous)
HIV
(AIDS)
HTLV-1
(ATL)
Overall approach: Genomics and Proteomics
- Standard Microarrays, siRNA, ChIP-Chip
- Virus Chip with TIGR
- Proteomics and Mass Spec
Drug studies and Membrane Proteome
- New animal model for HIV using Stem cells (In
collaboration with Dr. Saifuddin (Conrad))
Complex
Microarray
(Computer assisted data
analysis)
Gene
Narrow down to ~20-50 genes to define
individual pathways.
“Guilt by Association” for unknown genes or
for ~2 fold differences
Detection of protein complexes (Use of
conventional and immunoaffinity
chromatography)
Mass Spec to determine
individual partners
Protein
Biochemical/Functional
Assays
Simple
Localization
studies