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Bioenergetics Study Guide Bioenergetics – Overview - Use the PowerPoint from Dr. Chorney to understand what the overall idea of bioenergetics is. o Understand Gibbs Free Energy and spontaneous reactions o Slide 16 – Catabolism vs. Anabolism o What does an enzyme do? Glycolysis - What is it? - How does it work? (General overview – You will not need to have all the steps memorized, but be able to describe the steps if shown a diagram.) - What is used during glycolysis? - What is produced? Citric Acid Cycle - What is it? - How does it work? (General overview – You will not need to have all the steps memorized, but be able to describe the steps if shown a diagram.) - What is used during glycolysis? - What is produced? - How are glycolysis and the citric acid cycle related? Electron Transport Chain - What is it? - How does it work? (General overview – You will not need to have all the steps memorized, but be able to describe the steps if shown a diagram.) - What is used during glycolysis? - What is produced? - How are the electron transport chain and the citric acid cycle related? Enzyme Lab - What were the experiments in the enzyme lab and what happened in each? (Do not memorize the steps, but be able to explain the concepts and what you learned.) - How do genetic mutations affect enzyme catalysis? - Explain how enzymes are used in the production of ATP in glycolysis, citric acid cycle, and electron transport chain. - How do pH, concentration, and temperature affect the rate of enzyme activity? - Understand the information in the introduction and background portion of the lab.