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TCC, WEST CAMPUS VETERINARY TECHNOLOGY PROGRAM SYLLABUS ************************************************************************ COURSE: VT 2224 MICROBIOLOGY AND SANITATION CALL #: 13767 MEETS: Monday Wednesday 9:00 am – 11:50 am 9:00 am – 11:50 am Lecture/Lab Lecture /Lab ROOM: INSTRUCTOR: Jan Weaver, DVM Katherine Hill, RVT VTC VTC 595-8213 595-8218 DIVISION OFFICE: Dr. Bryan Coppedge Room S 128 595-8193 Assoc Dean, Science/Math TCC West Campus VTC VTC PREREQUISITE(S): All prior VT courses with a grade of "C" or better. COURSE DESCRIPTION: An introduction to microorganisms. Consideration will be given primarily to the pathogenic microorganisms related to veterinary medicine, including bacteria, viruses, fungi, and protozoa. Emphasis will be placed on sanitation, disinfectants, aseptic technique, sterilization techniques, zoonoses, and related public health concerns. Prerequisite: All prior VT courses with a grade of “C” or better. Lecture 3 hr, Lab 3hr. NEXT COURSE IN SEQUENCE: None DRESS CODE: Scrubs and appropriate footwear (no sandals, open toes,etc). Laboratory smocks and protective eyewear are required for all laboratory sessions. TEXTBOOKS AND SUPPLEMENTAL MATERIAL:: Bauman, et al, Microbiology, with Diseases by Taxonomy, 2nd(book, CD, study cards package) With Interactive student tutorial CD ROM; ISBN 32153934 Leboffe, Pierce, Microbiology Laboratory Theory and Application (Brief Ed); ISBN 0895827085 (OPTIONAL) Bauman, Study Guide for Microbiology (ISBN 0-8053-4887-5) Alcamo/Elson Microbiology Coloring Book (ISBN 0-06-041925-3) Ikram and Hill, Microbiology for Veterinary Technicians (ISBN 0-939674-30-0) <Out of Print> TEXTS ON RESERVE IN LRC: Bergey’s Manual of Determinative Bacteria (also available in VTC) Quinn, Carter, Clinical Veterinary Microbiology ISBN 0-7234-1711-3 Quinn, Markey Concise Review of Veterinary Microbiology (ISBN 1-405-10898-3) CAI (COMPUTER ASSISTED INSTRUCTION): DVD: Safety in the Microbiology Lab (14 min) Insight Media Videotapes: Care and Use of the Microscope (21 min) Carolina Biological Laboratory Safety CD ROM: The Microbiology Place, microbiology interactive student tutorial, (ancillary with text) Internet/web sites: The Microbiology Place, “Research Navigator”, pre-tests, practice tests, Microbe Reviews, links, glossary, videos, tutorials Other materials appropriate for this course Page 1 SUGGESTED RESOURCES: As suggested by Instructor. These may be found in the LRC on RESERVE. GENERAL EDUCATION GOAL STATEMENT: The General Education Goals are designed to ensure that graduates of Tulsa Community College have the skills, knowledge, and attitudes to carry them successfully through their work and their personal lives. General Education Goals relevant to this course include: Critical Thinking; Effective Communication-written and verbal; Civic Responsibility. OBJECTIVES: The student will gain an understanding of microbiology, including bacteriology, mycology, virology and immunology, and properly perform diagnostic procedures and appropriately record their findings. Students should understand the theory and demonstrate the following: Use and Care of the microscope Naming and classification of microorganisms Components and structures of microorganisms Biochemistry of microorganisms Microbial genetics Obtaining and preparing microbiological samples Culturing and growth requirements of microorganisms Control and sanitation of microorganisms Principles of disease and epidemiology Principles of Immunology AVMA ESSENTIAL & RECOMMENDED TASK LIST Required tasks are denoted by an asterisk (*). 6. LABORATORY PROCEDURES Specimen management Skill: Properly package, handle and store specimens for laboratory analysis Tasks: Prepare specimens for diagnostic analysis* Select and maintain laboratory equipment* Implement quality control measures* [GROUP] Ensure safety of patients, clients and staff* Decision-making abilities: 1. Given the characteristics of the patient and the requested analysis, the veterinary technician will properly prepare, handle and submit appropriate samples for diagnostic analysis in order to ensure maximum accuracy of results. 2. Given the characteristics of laboratory instruments and equipment, the veterinary technician will determine proper maintenance and quality control procedures necessary to ensure accurate results. Skill: Properly carry out analysis of laboratory specimens. Tasks: Perform microbiologic procedures/evaluations: collect representative samples* culture bacteria and perform sensitivity tests* identify common animal pathogens using commercially available media and reagents* [GROUP] collect milk samples and conduct mastitis testing (e.g., CMT, bacterial culture) perform common biochemical tests* [GROUP] perform staining procedures* culture and identify common dermatophytes* Decision-making abilities: 1. Given the characteristics of the patient, the specimen submitted and the results of the analysis, the veterinary technician will be able to recognize accurate vs. erroneous results in order to provide maximum diagnostic benefit. 2. Given the laboratory specimen collected and characteristics of the patient, the veterinary technician will determine appropriate methodology and carry out analytical procedures necessary to provide accurate and precise diagnostic information. 3. Having determined the accuracy of analytical results, the veterinary technician will work with the veterinarian to determine if a need exists for additional laboratory tests that will provide useful diagnostic information. Page 2 TEACHING METHODS: Lecture, Laboratory and hands-on practice of theory, TCC Blackboard academic suite, plus any other methods deemed suitable by instructor. Field trips: Transportation to and from field trips and any off-site locations are the student's responsibility. EVALUATIONS AND GRADING: Student attendance will be taken each class and each lab period. Your participation in discussion and laboratory will contribute to your final grade. Additionally, your participation, completion and submission of “Incubation” and “Growth” activities via Blackboard (online) will also contribute to your final grade. GRAND ROUNDS – Scores from Grand Rounds will accrue to this course. Remember: you earn GR points by attendance and participation! LECTURE -- Students will be evaluated by (5) regularly scheduled tests of 100 points each covering lecture and laboratory. Comprehensive Final Examination worth 200 points, covering lecture and laboratory. In addition to the examinations, each student is required to complete a scientific research paper of between 3 and 5 pages and present it to the class. Focus: the description and characteristics of a specific organism of veterinary medical importance. Topics will be chosen by the student and approved by the instructor. Students will sign-up for presentation date, presentations during weeks 4-14. The paper shall be in APA format, double-spaced, 1 inch margins, font size 12 Times New Roman and is worth 100 points. Papers not adhering to APA format will be rejected for student rewrite and will automatically lose 1 letter grade. Your presentation must have visual aids: powerpoint, poster, handouts or other demonstration material. References: You must have at least 2 textbooks and at least 1 reputable journal article in addition to other references. LABORATORY -- Demonstration to the instructor’s satisfaction of laboratory tasks and procedures. Lab sessions will be worth 10 points each. Students will earn lab points through participation, completion/submission of weekly laboratory reports and instructor evaluation of weekly laboratory activities (growth/test results). Each student will complete one Unknown Identification, worth 50 points. There is 1 practical laboratory test covering laboratory information and techniques, worth 100 points. There are 50 points allotted to unannounced quizzes and/or BlackBoard (BB) discussions. Field Trips will have 1-page papers, each worth 50 points. LECTURE POINTS POSSIBLE LAB PARTICIPATION(30 labs) LAB UNKNOWNS & Practicals FIELD TRIP & PAPERS QUIZZES, BlackBoard GRAND ROUNDS TOTAL POINTS POSSIBLE 800 300 300 100 50 50 90% + 80 – 89% 70 – 79% 60 – 69% < 60% A B C D F 1,600 MAKE-UP AND LATE ASSIGNMENTS POLICY Make-up for lecture examinations must occur within 1 week of the scheduled examination, or with permission of the Instructor. Make-up examinations may be of a different style, (for example, all essay questions) at the Instructor's discretion and are taken in the Academic and Campus Services (ACS) office, I-106. Make-up for missed laboratory sessions are at the Instructor’s discretion. (Some labs simply cannot be duplicated). Students must arrange with the Instructor to make-up lab activities. Lab reports remain due regardless of whether you were able to perform the lab or not, due date at the Instructor’s discretion. Unknowns will be performed or student will receive a “0” score. THERE WILL BE NO MAKE-UP OF LABORATORY PRACTICAL EXAMINATIONS. Late Assignments require approval by the Instructor. You will automatically lose 1 grade level for all late assignments (ex. assignment will receive no higher than a grade of "B"). Page 3 COMMUNICATION with the Instructor is the key. I emphasize contacting the Instructor as soon as you know of a potential problem. GENERAL POLICIES: 1. TCC prohibits smoking or the use of other tobacco products within all College-owned or leased buildings. 2. Eating and/or drinking is not permitted in the classroom, unless specified by your physician and Student Health Services is apprised. 3. The College student is considered a responsible adult. The student’s enrollment indicates acceptance of those standards of conduct which appear in the student handbook and activities calendar. 4. Dropping / Withdrawal policy – as specified in the College Catalog. Consult this Semester’s Calendar. Please meet with instructor(s), Program Coordinator and/or Counseling prior to dropping or withdrawing from any course in the Veterinary Technology Program. 5. Appropriate dress is required for each course. Consult the instructor about the specific dress code for this course. 6. Students should be on time to class out of respect to other class members and the learning process. 7. Any incidence of cheating or academic dishonesty will result in a grade of zero on that assignment/exam and the assignment/exam will not be dropped, and incident will be referred to the Dean of Student Services and/or Dean of Instruction for further action. The second incidence of cheating or academic dishonesty will result in an “F” for the course. 8. Late assignments / make-ups policies – Late assignments will not be accepted, unless previously arranged with the instructor. Make-up tests must be arranged in advance with the instructor. In case of emergency absences, the student must make arrangements with the instructor and Program Coordinator. ATTENDANCE Please refer to the General Policies. Attendance will be taken at every lecture and lab. Anticipated absences must be arranged with the instructor and/or Program Coordinator. Regular class attendance is not only important from the standpoint of learning, but also is a must in order to be successful in this course. Students are held responsible for all materials covered during their absence and any changes made in the agenda. An Excessive Absence notice will be issued after a student misses the equivalent of two class periods and is not maintaining a “C” average for the class. The Excessive Absence notice will automatically notify the Financial Aid office and Veterans Services, if applicable. The student may also be subject to Administrative Withdrawal (AW) for non-attendance. WITHDRAWAL, AUDIT, AND INCOMPLETE POLICY To withdraw from the class, the student should initiate an official withdrawal through the counseling office; nonattendance DOES NOT constitute official withdrawal. Failure to withdraw may result in the student receiving a regular grade of “F” at the end of the semester. To request a change to Audit (A) or Incomplete (I), the student must be maintaining a passing grade. The student must initiate the change with the instructor and sign the audit or incomplete agreement. If the Incomplete is due to a circumstance that prevents the student from being able to sign the form him/herself, the instructor may make other arrangements. The last date to withdraw or change to audit for the Fall 2006 semester is Nov. 10, 2006. READING & WRITING TUTORING SERVICES The Technology Learning Center’s Reading & Writing Services helps student’s build analytic reading, solid research and effective writing skills. Additionally, Reading & Writing Services can supplement classroom instruction with information on MLA, APA, or Chicago Manual styles, Internet research strategies, source evaluation and Page 4 documentation methods, and essay writing techniques. Hours: Monday-Thursday 8:00 am – 8:00pm; Friday 8:00am – 5:00pm; Saturday 8:00am – 1:00pm. (subject to change) INCLEMENT WEATHER AND CLASS CANCELLATION POLICY When severe winter weather hits, you may assume that TCC will be open for classes unless it is announced otherwise on television, radio stations and on the TCC web site. TCC does not automatically close if Tulsa Public Schools close. If your instructor decides individually to cancel class because of inclement weather or other unexpected reasons, your instructor will contact you. It is important that your instructor have current contact information so you can be notified if necessary. Notify the TCC Registrar’s office and your instructor if your contact information changes during the semester. PLAGIARISM POLICY Deliberate plagiarism is claiming, indicating, or implying that the ideas, sentences, or words of another writer are your own; it includes having another writer do work claimed to be your own, copying the work of another and presenting it as your own, or following the work of another as a guide to ideas and expression that are then presented as your own. At the instructor’s discretion, a student guilty of deliberate plagiarism may receive a zero for the assignment and an “F” in the course. Accidental plagiarism is the handling of quotations and paraphrases without a deliberate attempt to deceive; it includes failing to mark the beginning of paraphrases, failing to get away from the language of the original text when paraphrasing, failing to mark quotations with properly placed quotation marks, and failing to properly identify the source of a quotation or paraphrase. At the instructor’s discretion, a student whose paper contains accidental plagiarism may have the opportunity to rewrite the paper with a reduction in grade. ACADEMIC DISHONESTY OR MISCONDUCT Academic dishonesty or misconduct is not condoned nor tolerated at campuses within the Tulsa Community College system. Academic dishonesty is behavior in which a deliberately fraudulent misrepresentation is employed in an attempt to gain undeserved intellectual credit, either for oneself or for another. Academic misconduct is behavior that results in intellectual advantage obtained by violating specific standard, but without deliberate intent or use of fraudulent means. Academic dishonesty or misconduct cases are governed by the Campus Student Rights and Responsibilities Code (see Student Conduct Handbook). COMPUTER SERVICES ACCEPTABLE USE Access to computing resources is a privilege granted to all TCC faculty, staff, and students. Use of TCC computing resources is limited to purposes related to the College’s mission of education, research, and community service. Student use of technology is governed by the Computer Services Acceptable Use Statements/Standards found in the TCC Student Code of Conduct Policy Handbook. Handbooks may be obtained by contacting any Student Activities or Dean of Student Services office, or Online at www.tulsacc.edu, http://www.tulsacc.edu/page.asp?durki=2984 CLASSROOM ETIQUETTE All classroom and lab areas are to be cleaned and organized before leaving class. Cell phones are to be turned off during lab and lecture unless special arrangements are made. INSTITUTIONAL STATEMENT Each student is responsible for being aware of the information contained in the TCC Catalog, TCC Student Handbook, and semester information listed in the Class Schedule. Page 5 LECTURE SCHEDULE Week Date 4-14 1 Topic Reading Student Presentations – one/two per week 18Aug08 Introduce Syllabi Introduction to Microorganisms and History Classification and ID of Microorganisms Presentations (10 min each) B: Ch 1, Ch 4 p 112-end SG: Ch 1 IH: Ch 1 Mc: Ch 8 {Read Mc Ch8 ALL THRU course} 20Aug08 Chemical properties B: Ch 2 Osmolarity and Transport B: Ch 3 p 69-73, Ch 6 p 173-4 B: Ch 3; Ch 4 p 112-end SG: IH: Ch 3 p 11-21 ** READ ** ** READ ** ** READ ** B: Ch1-4, Ch 11, Ch 6 p 174-184, Ch 11 p315-26, Appdx A IH: Ch 3-4 Physical Characteristics-Structure and Function 2 25Aug08 3 01Sep08 (Labor Day Monday Sept 1 – NO CLASS) 4 5 6 (Wed) 03Sep08 ----Structure and Function (cont’d) Microscopy, Staining and Classification B: Ch 3, Ch 4 (all), Ch 6 p 174-184, Ch 11 p315-26, Appdx C IH: Ch 2, 3 & 4, Ch 5 08Sep08 EXAM #1 (week 1-3) Microbial Metabolism B: Ch 5, Appendix A (pathways), Appendix B SG: IH: Ch 4 p 22-27 15Sep08 Microbial Nutrition and Growth Microbial Genetics Recombinant DNA and Biotechnology B: Ch 6 p 165-174, p 184-end, Ch 7, Ch 8 SG: IH: Ch 5 22Sep08 EXAM # 2 (Weeks 4-5) Pathogenic Gram + Bacteria B: Ch 11 p 326-end, Ch 19 SG: IH: Ch 6,7,8 Pathogenic Gram – Bacteria B: Ch 11 p 326-end, Ch 20 SG: IH: Ch 9-17 (9 chapters, 44 pgs) 24Sep08 Page 6 7 29Sep08 Pathogenic Gram – Bacteria B: Ch 11 p 326-end, Ch 20 SG: IH: Ch 9-17 (9 chapters, 44 pgs) 8 06Oct08 Mycoplasmas, Rickettsias, Chlamydias, Spirochetes, Vibrios B: Ch 21 HO: organisms of VM importance 13Oct08 EXAM # 3 (week 6-8) Characterization & Classification of Eukaryotes Pathogenic Fungi and Mycology –Superficial & Systemic B: Ch 3 p77-end, Ch 12, Ch 22 SG: IH: Ch 18 – 26 (9 chapters, 34 pgs) Mc: Ch 8 (p170-171) 20Oct08 Characterization & Classification of Viruses, Viriods, and Prions Pathogenic DNA Viruses Pathogenic RNA Viruses B: Ch 13, Ch 24, Ch 25 HO: organisms of VM importance IH: Ch 27 – 30 (4 chapters, 18 pgs) Mc: Ch 8 (p171-172) 11 27Oct08 EXAM # 4 (week 9-10) Controlling Microbial Growth Sanitation B: Ch 9, Ch 10 SG: Mc: Ch 8 (Nosocomial inf to end), Ch 22 p 692-703 12 03Nov08 Controlling Microbial Growth Sanitation HO: Dr. Weaver’s Notes Mc: Ch 8 (Nosocomial inf to end), Ch 22 p 692-703 13 10Nov08 Controlling Microbial Growth – Antibiotic Sensitivity B: Ch 10 SG: 17Nov08 EXAM # 5 (week 11-13) Infection and Epidemiology (Principles of Disease, Epidemiology, Microbial Pathogenicity) B: Ch 14, Appdx D SG: Mc: (Wed) 19Nov08 Innate Immunity Adaptive Immunity B: Ch 15, Ch 16 SG: 24Nov08 Immunization and Immune Testing Hypersensitivities, Autoimmune Diseases and Immune Deficiencies B: Ch 17, Ch 18 SG: Mc: Ch 8 (p172-178 Serology-Nosocomial) 9 10 14 15 Page 7 (Wed) 26Nov08 16 01Dec08 Wed Nov 26 – Thanksgiving – No Class Student-Guided Review Microorganisms and Animal Disease – selected topics 17 08Dec08 COMPREHENSIVE FINAL EXAMINATION Mc: Ch8 (p162-186 including Mycoplasma) ** Lecture & Lab Material ** Reading Abbreviations: xxxxx xxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx Extra unscheduled Field Trip - Wastewater Treatment Plant Applied and Environmental Microbiology Field Trip – Microbiology Laboratory Paper Presentations (10 min each) B: Bauman, Microbiology with Dz by Tax. (textbook) SG: Study Guide IH: Ikram and Hill, Microbiology for Vet Techs Mc: McCurnin, Clinical Textbook for Vet Techs xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx B: Ch 26 TBD Page 8 LABORATORY SCHEDULE Week Date Topic Laboratory Safety Lab Orientation, Syllabus and Lab Manual “cruise” Reading L: Introduction p 1-5; HO’s DVD: Safety In the Microbiology Laboratory (14 min) L: Introduction p 5-6; Sect. 1 Fundamental Skills p 7 Experimental Design L: p 7, Ex 1-5 HO: Sampling Hx: Ch 4 p 105-112 Sampling 1a 18Aug08 3-1 Light Microscope Orientation, Proper cleaning, Proper storage Assign Lab Drawers and Smock Storage Video: Use and Care of the Microscope (23 min) L: Ex 3-1 (prepared slides- parasites & bacteria) 1-1 Hand Wash Education (dry lab) 1-2 Media Preparation (dry lab) Core Technique: Measures and Weighing, Reading the Meniscus L: p 7, Ex 1-1; 1-2, Apdx B IH: Ch2 p 5-6 HO: Core techniques Collaboration and Teamwork Discussion 2-1 Ubiquity of Microorganisms & EnvironmentalSampling 1b 20Aug08 ****** Thursday 1-3 Common Aseptic Transfers and Inoculations CT: Stirring broth tubes (vortex mixer and by hand), Aerosol avoidance READ: 2-1, 1-3 L: 2-1 IH: Ch5 p 29-39 L: 1-3 Hx: Ch 4 Diagnostic Microbiology, p128-32, 138-41 READ: 2-1, 1-3 – Collaborate w/ colleagues, Share data L: p47, 2-2, 2-3, 2-4; 2a 25Aug08 2b 27Aug08 3a 1Sep08 CT: Positive and Negative Controls 2-2 Colony Morphology (use 2-1 plates) 2-3 Growth Patterns on Slants } 2-4 Growth Patterns on Broth } (use 1-3 tubes) 3-3 Examination of Eukaryotes (pond H20) READ: (previous exercises) p119, 3-3, 3-10 ** 3-10 Omit Hanging Drop section HO’s Hx: Ch 4 Diagnostic Microbiology {pond H2O, Bacillus, E.coli, Serratia) Discuss: Dr. W’s “Trilogy” morphology/motility/Gram stain L: 3-4, 3-5 ** Ex 3-5: Make 2 slides – 1 heavy inoculum, 1 thin Hx: Ch 4 Diagnostic Microbiology 3-10 Wet Mounts and MOTILITY 3-4 Simple Stains 3-5 Negative Stains LABOR DAY READ READ READ READ READ READ READ READ Page 9 3b 3Sep08 4a 8Sep08 4b 10Sep08 1-4 Streak Plate Methods of Isolation (Isolation by Dilution Technique) Discuss Data Sheets and Lab Reports, Progress to date READ: 1-4 plates L: 1-4 3-6 Gram Stain Questions??? L: 3-6 (Hint: Colony morphology 2-2) Hx: Ch 4 Diagnostic Microbiology, p105-214 IH: Gram stain Nutrition: 2-5 Evaluation of Media (nutritional requirements) 5-20 BAP vs TSA (Blood Agar Plate vs Tryptic Soy Agar) L: p 47, 2-5, 5-20 HO – BAP (blood agar plate), TSA (tryptic soy agar), NA (nutrient agar plate) READ: 2-5, 5-20, Gram Stain – 1 from each plate 5a 5b ****** L: p 225, 5-1, 2-6 17Sep08 Metabolic Pathways: Cellular Respiration vs Fermentation 5-1 Oxidation / Fermentation Test (O/F Tubes) 2-6 Fluid Thioglycollate Medium READ: 5-1, 2-6 Metabolic Pathways: Cellular Respiration vs Fermentation 5-4 Catalase Test 5-5 Oxidase Test Carbohydrate Catabolism 5-2 Phenol Red Broths Thursday READ: 5-2 ** Gram stain Turn In ** Practice Motility and Gram stains – Use PR broths; Turn in student slides to Dr.W & Katherine properly ID’d Metabolic Pathways: Protein catabolism 5-3 MRVP Methyl Red / Voges-Proskauer 5-12 Urease Test 5-14 Gelatinase Test (Gelatin Hydrolysis/catabolism) 5-8 Decarboxlylation (amino acid catabolism) L: 5-3, 5-12, 5-14,5-8 Hx: Ch 4 Diagnostic Microbiology, p105-214 15Sep08 6a 22Sep08 ****** Tuesday ?? Questions about Lab Reports ?? L: 5-4, 5-5; 5-2, 5-11 Hx: Ch 4 Diagnostic Microbiology, p105-214 IH: Catalase & Oxidase tests, Carb metabolism (MR/VP requires 48 hrs, others exercises may require 48hr + incubation time; decarbox & gelatin 5-7 days) ** Lots to inoculate !! ORGANIZE and PLAN !! ** Practice Gram stains – Use Urease tubes READ: 5-12 Urease READ: 5-3 MRVP CHO and Protein Metabolism, final pH, Energy Pathways and inducible pathways, Production of proteins (enzymes) and genetic info need to produce them 6b 24Sep08 7a 29Sep08 QUIZ next Monday – up to this point including today’s Basic techniques and stains, Identification and explanation of test results Discuss ?’s to date – Student Guided Review Basic techniques and stains, Identification & explanation of test results (up to this point including week 6 labs) *** LAB PRACTICAL *** Page 10 ****** Tuesday READ: 5-14 Gelatinase, 5-8 Decarboxylations ** Lots to inoculate !! ORGANIZE and PLAN !! Discuss Differential vs Selective 7b ****** 1Oct08 Thursday L: p 197, 4-1, 4-2, 4-4, 4-6 Each student will streak all 4 plates with Differential Media: Read and review p 197, 4-3, 4-5 (omit procedures, any ??’s) (Some of these media have multiple biochemical reactions occurring—be sure to record them ALL.) Differentiation of Bacteria: Selective vs Differential Media 4-1 Mannitol Salt Agar (MSA) 4-2 Phenylethyl Alcohol Agar (PEA) 4-4 Eosin Methylene Blue Agar (EMB) 4-6 MacConkey Agar (Some of these media have multiple biochemical reactions occurring—be sure to record them ALL.) READ: (previous labs) Remember TEAMWORK !! READ: (previous labs) L: 5-21, 5-10, 5-19 review 5-20 Blood Agar Plate (review hemolysis); 4-1 Mannitol Salt; 4-2 PEA L: p 455, 9-2 & 9-6 Demonstration, HO Bergey’s Group 17 Staphylococcus, Streptococcus (Lancefield groups) & S.bovis, S.pyogenes, S.equi, Enterococcus, Ruminococcus Group 18 Clostridium, Bacillus Group 19 Erysipelothrix, Listeria Group 20 Actinomyces, Bifidoacterium, Corynebacterium Group 22 Nocardia, Rhodococcus (R.equi-formerly Coryne) Group 21 Mycobacteria Gram Positive Cocci and Rods: 5-21 Coagulase Test 5-10 Bile Esculin Test (dry lab) 5-19 Bacitracin Disc (on BAP) 9-2 Identification of G(+) Cocci CAMP Test PYR Test (dry lab) Optochin Test 9-6 ID Streptococci by Latex Agglutination (dry lab) 8a 6Oct08 READ: (previous labs) 8b 8Oct08 13Oct08 9a ****** Tuesday L: 3-8, 5-4 IH: structural stains Structural stains: 3-8 Capsule Stain 5-4 Catalase test ** Gram Stains < Continue G(+) Bergey’s Groups > L: all applicable exercises, “Trilogy”, API HO’s, Bergey’s Manual and other references, (oxidase, catalase, capsule stain) < Continue G(+) Bergey’s Groups > Gram Positive Cocci: API Staph API Strept DDx: Staph vs Strept DDx: Staph spp and Strept spp ** API Strept – read some tests in 4 hours ** Hosp II lab – obtain gingival pocket inoculums & set up anaerobic cultures (or, you may use your own gingiva for next lab) Page 11 Gram Positive Rods (and Cocci): Endospore vs. Non-Endospore Forming Rods L: 3-9, 2-7 Group 18: G+ endospore rods: Bacillus, Clostridium Group 19: G+ non-endo rods: Erysipelothrix, Lactobacillus, Listeria Group 20: Pleomorphic non-endo rods: Actinomyces, Bifidobacterium, Corynebacterium, Propionibacterium Group 22: Pleomorphic non-endo rods: Rhodococcus (formerly Corynebacterium) L: 3-7 Bergey Group 21-22: Mycobacterium, Nocardia, Actinomyces Other Acid-Fast organisms: Rhodococcus +/- weak positive, Protozoan oocysts – Cryptosporidium, Isospora Others: Dermatophilus, Streptomyces 3-9 Endospore stain 2-7 Anaerobic Environment (& 2-6) (oral gingival pocket inoculum) 9b 15Oct08 Acid Fast Microorganisms 3-7 Acid Fast Stain 10a 10b 20Oct08 22Oct08 (“Gram+” because they don’t decolorize well) Gram Negatives: part 1 Enterobacteriaceae 5-18 Kligler’s Iron Agar (&Triple Sugar Iron Agar (TSI) 5-6 Nitrate Reduction 9-1 IMViC tests and Enterobacteriaceae ID (IMViC is dry lab) L: p455 5-18, 5-6, 9-1 (These media have multiple biochemical reactions occurring—be sure to record them ALL.) ****** Thursday READ: 5-18 Kligler’s, 5-6 NO3 ** Schedule your time for reading and recording !! Thursday ACR Team –collect 5 fecal samples (Ca, Fe, Lg an, lab an) Katherine: Thioglycollate and TSB inoculation Place properly ID’d samples in lab for Katherine ****** Friday Katherine: Streak plates for isolation MacConkey and BAP Inoculum = TSB (mix the tube before sampling) ****** ** prepare for Monday’s lab L: 5-5, 9-4, 9-5 ** Each student will inoculate 1 Rapid ID test with known bacterium ** Each table will inoculate 1 Rapid ID test with your choice from your poop plate (think about which Rapid ID test you will use & why, based upon “Trilogy”, oxidase tests – must be approved by Dr. W.) DUE DATE: __________________ READ: (previous labs) + poo poo platters Rapid Identification Systems 5-5 Oxidase test on selected bacteria (see Dr. W) 9-4 API 20E 9-5 Enterotube Bergey’s Group 5 (Subgroup 1-important genera):Escherichia, Enterobacter, Klebsiella, Salmonella, Shigella, Proteus, Serratia, Yersinia 11a 27Oct08 ** must read Rapid ID’s within 24 hours ** (SAVE poop plates for next lab) Page 12 READ Rapid ID tests ****** Tuesday Schedule enough time to add reagents, reaction times for tests and to record your results Optional testing for Enterobacteriaceae; Others available – see Dr. W to help with differentiation and ID ONPG Test , XLD agar, SS Agar READ: (previous labs) + poo poo platters L: In-house OxiFerm tubes (all other pertinent labs and references), HO Gram Negatives, part 2: Other than Enterobacteriaceae ID procedures: “Trilogy”, oxidase, catalase, TSB (O2 req’s), MacConkey, BAP, O/F tubes Bergey’s Group 5: (Subgroups 2,3) Rods straight/curved: Aeromonas, Plesiomonas, Vibrio Pleomorphic: Actinobacillus, Pasteurella-Burkholderia, Mannheimia-Hemophilus Bergey’s Group 4 – Aerobic and Microaerophilic Gram(-): Rods: Pseudomonas, Francisella, Rochalimaea-Bartonella, Bacteroides Coccobacilli or pleomorphic: Bordetella, Brucella, Moraxella, Branhamella, Taylorella, (Neisseria) Others: Chlamydophila (aka Chlamydia), Porphyromonas Curved/Helical: Campylobacter, Helicobacter Spirochetes: Leptospira, Serpulina-Treponema, Borrelia 5-5 Oxidase test on selected bacteria (see Dr. W) 9-4 API 20E In-house Lab: OxiFerm tubes 11b ****** 12a ****** 29Oct08 Thursday 3Nov08 Tuesday READ Rapid ID tests (schedule enough time to add reagents, reaction times for tests and to record your results, due Oct 23) 7-2 Antimicrobial Susceptibility Test (Kirby-Bauer) L: 7-2 Read 7-2, measure zones w/in 24 hours (mm’s) (Schedule enough time to record zones for all discs) L: Read 7-5, 7-6 and textbook Ch26 BEFORE trip (not procedures) Field Trip – Water Treatment Plant 12b 5Nov08 Meet at VTC and carpool, special arrangements for those living close to Plant – see Dr. W ** 1 page paper due MON Nov 10 ** Page 13 L: p 455, all applicable exercises, Lab section 9, Bergey’s Manual and other Keys You must decide which Rapid ID test you will use, based upon “Trilogy”. All oxidase tests – must be approved by Dr. W. Unknown # 1 with Antibiotic Susceptibility 13a 10Nov08 13b 12Nov08 Lab Report: 9-1,2,3 + Rapid ID sheet + HO (explanation of tests and support of your ID) + Report of Susceptibility due Nov 17 (schedule enough time to add reagents, reaction times for tests and to record your results, due Nov 17) L: 2-6, 2-7, 2-8--HO READ Rapid ID tests Control of Growth: 2-6 & 2-7 Oxygen as Growth Control (dry lab discussion) 2-8 Effect of Temperature on Microorg’s 14a 17Nov08 Control of Growth: 2-9 Effect of pH on Microorg’s 2-10 Effect of Osmotic Pressure on Microorg’s 2-11 Lethal Effect of UV Light on Microorg’s L: 2-9, 2-10, 2-11, 2-12 Tables 1 & 2 Ex 2-9 } Collaboration: Share your results Tables 3 & 4 Ex 2-10 } with ALL classmates – HINT: for Tables 5 & 6 Ex 2-11 } Final Exam !! 2-12 Effectiveness of Hand Washing Use-Dilution Test (dry lab discussion) (inoculum = rub your face, nose, forehead with your inoculating fingertips) L: 2-12 (ALL Tables perform) HO: USE DILUTION Lab 14b 19Nov08 2-13 Steam Sterilization ****** Tuesday READ: (previous labs) 2-13 Retrieve and incubate ampules 24Nov08 15b 26Nov08 L: 2-13 (demonstration-place inside surg pack-autoclave and use pack Tues—incubate Tuesday ) L: 3-3; HO (fungal ID keys) Yeast: Malessezia, Candida, Saccharomyces Mold: Penicillium, Trichophyton, Microsporum, Aspergillus, Mucor, Rhizopus, Fusarium L: Appendix C, D + HO Mycology: 3-3 The Fungi-Common Yeast and Molds CT: Pipetting 15a ** Bring Soap or Sanitizer of your choice for next lab Ex 2-12 ** ** Bring liquid disinfectant & manufacturer’s directions for dilution -- for next lab ** Thanksgiving Vacation (prehension, mastication, deglutition, digestion) Page 14 ** Lab first, then Lecture, then Lab to read results ** 16a 1Dec08 ***** Tuesday 16b 3Dec08 17a 8Dec08 Molecular Biology: Electrophoresis HO: Electrophoresis, OSU “Footlocker” Ex - Electrophoresis Epidemiology 7-3 MMWR Report 7-4 Epidemic Simulation L: 7-3, 7-4, CDC Website—MMWR reporting, OK State Dept of Health Report Review: CMT Californial Mastitis Test HO: CMT Field Trip - Dairy or Diagnostic Lab (TBA) California Mastitis test HO: California Mastitis test Check in Lab Drawers Final Lab Cleaning Reading Abbreviations: L: Leboffe & Pierce, Microbiology Laboratory Theory , and Application, Brief Edition (lab book) IH: Ikram, Hill Microbiology for Vet Techs Hx: Hendrix, Laboratory Procedures for Vet Techs HO = Hand Out (“stuff” from Dr W) CT = Core Technique xxxx xxxxxxxx Extra Labs xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx Quantitative Techniques: 6-1 Standard Plate Count 6-2 Urine Culture 6-3 Direct Count LAB FINAL 3-2 Calibration of the Ocular Microscope 3-12 Morphologic Unknown 3-12 and perform ID stains xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx L: 6-1, 6-2, 6-3, HO-CMT Mastitis & Milk Water-borne Illness (coliforms) Food-borne illness (Listeria, coliforms, Staph, Strept) Basic techniques, Gram Stain, Selective & Differential media, Interpretation of test results, Characteristics of major organisms (Ex. E.coli, Pseudomonas, Clostridium, etc) 5-11 Starch Hydrolysis Page 15