Download EXAM # 3 (week 6-8) - Blackboard Learn

TCC, WEST CAMPUS
VETERINARY TECHNOLOGY PROGRAM
SYLLABUS
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COURSE: VT 2224
MICROBIOLOGY AND SANITATION
CALL #: 13767
MEETS:
Monday
Wednesday
9:00 am – 11:50 am
9:00 am – 11:50 am
Lecture/Lab
Lecture /Lab
ROOM:
INSTRUCTOR:
Jan Weaver, DVM
Katherine Hill, RVT
VTC
VTC
595-8213
595-8218
DIVISION
OFFICE:
Dr. Bryan Coppedge
Room S 128
595-8193
Assoc Dean, Science/Math
TCC West Campus
VTC
VTC
PREREQUISITE(S): All prior VT courses with a grade of "C" or better.
COURSE DESCRIPTION:
An introduction to microorganisms. Consideration will be given primarily to the pathogenic microorganisms related to
veterinary medicine, including bacteria, viruses, fungi, and protozoa. Emphasis will be placed on sanitation,
disinfectants, aseptic technique, sterilization techniques, zoonoses, and related public health concerns. Prerequisite:
All prior VT courses with a grade of “C” or better.
Lecture 3 hr, Lab 3hr.
NEXT COURSE IN SEQUENCE: None
DRESS CODE:
Scrubs and appropriate footwear (no sandals, open toes,etc). Laboratory smocks and protective eyewear are required
for all laboratory sessions.
TEXTBOOKS AND SUPPLEMENTAL MATERIAL::
Bauman, et al, Microbiology, with Diseases by Taxonomy, 2nd(book, CD, study cards package)
With Interactive student tutorial CD ROM; ISBN 32153934
Leboffe, Pierce, Microbiology Laboratory Theory and Application (Brief Ed); ISBN 0895827085
(OPTIONAL)
Bauman, Study Guide for Microbiology (ISBN 0-8053-4887-5)
Alcamo/Elson Microbiology Coloring Book
(ISBN 0-06-041925-3)
Ikram and Hill, Microbiology for Veterinary Technicians (ISBN 0-939674-30-0) <Out of Print>
TEXTS ON RESERVE IN LRC:
Bergey’s Manual of Determinative Bacteria
(also available in VTC)
Quinn, Carter, Clinical Veterinary Microbiology ISBN 0-7234-1711-3
Quinn, Markey Concise Review of Veterinary Microbiology (ISBN 1-405-10898-3)
CAI (COMPUTER ASSISTED INSTRUCTION):
DVD:
Safety in the Microbiology Lab (14 min) Insight Media
Videotapes:
Care and Use of the Microscope (21 min) Carolina Biological
Laboratory Safety
CD ROM:
The Microbiology Place, microbiology interactive student tutorial, (ancillary with text)
Internet/web sites: The Microbiology Place, “Research Navigator”, pre-tests, practice tests, Microbe Reviews,
links, glossary, videos, tutorials
Other materials appropriate for this course
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SUGGESTED RESOURCES: As suggested by Instructor. These may be found in the LRC on RESERVE.
GENERAL EDUCATION GOAL STATEMENT:
The General Education Goals are designed to ensure that graduates of Tulsa Community College have the skills,
knowledge, and attitudes to carry them successfully through their work and their personal lives. General Education
Goals relevant to this course include: Critical Thinking; Effective Communication-written and verbal; Civic
Responsibility.
OBJECTIVES:
The student will gain an understanding of microbiology, including bacteriology, mycology, virology and immunology,
and properly perform diagnostic procedures and appropriately record their findings.
Students should understand the theory and demonstrate the following:
 Use and Care of the microscope
 Naming and classification of microorganisms
 Components and structures of microorganisms
 Biochemistry of microorganisms
 Microbial genetics
 Obtaining and preparing microbiological samples
 Culturing and growth requirements of microorganisms
 Control and sanitation of microorganisms
 Principles of disease and epidemiology
 Principles of Immunology
AVMA ESSENTIAL & RECOMMENDED TASK LIST Required tasks are denoted by an asterisk (*).
6. LABORATORY PROCEDURES
Specimen management
Skill: Properly package, handle and store specimens for laboratory analysis
Tasks:
 Prepare specimens for diagnostic analysis*
 Select and maintain laboratory equipment*
 Implement quality control measures* [GROUP]
 Ensure safety of patients, clients and staff*
Decision-making abilities:
1. Given the characteristics of the patient and the requested analysis, the veterinary technician will properly prepare, handle
and submit appropriate samples for diagnostic analysis in order to ensure maximum accuracy of results.
2. Given the characteristics of laboratory instruments and equipment, the veterinary technician will determine proper
maintenance and quality control procedures necessary to ensure accurate results.
Skill: Properly carry out analysis of laboratory specimens.
Tasks:

Perform microbiologic procedures/evaluations:
 collect representative samples*
 culture bacteria and perform sensitivity tests*
 identify common animal pathogens using commercially available media and reagents* [GROUP]
 collect milk samples and conduct mastitis testing (e.g., CMT, bacterial culture)
 perform common biochemical tests* [GROUP]
 perform staining procedures*
 culture and identify common dermatophytes*
Decision-making abilities:
1. Given the characteristics of the patient, the specimen submitted and the results of the analysis, the veterinary technician will be
able to recognize accurate vs. erroneous results in order to provide maximum diagnostic benefit.
2. Given the laboratory specimen collected and characteristics of the patient, the veterinary technician will determine appropriate
methodology and carry out analytical procedures necessary to provide accurate and precise diagnostic information.
3. Having determined the accuracy of analytical results, the veterinary technician will work with the veterinarian to determine if
a need exists for additional laboratory tests that will provide useful diagnostic information.
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TEACHING METHODS:
Lecture, Laboratory and hands-on practice of theory, TCC Blackboard academic suite, plus any other methods deemed
suitable by instructor.
Field trips: Transportation to and from field trips and any off-site locations are the student's responsibility.
EVALUATIONS AND GRADING:
Student attendance will be taken each class and each lab period. Your participation in discussion and laboratory will
contribute to your final grade. Additionally, your participation, completion and submission of “Incubation” and
“Growth” activities via Blackboard (online) will also contribute to your final grade.
GRAND ROUNDS – Scores from Grand Rounds will accrue to this course. Remember: you earn GR points by
attendance and participation!
LECTURE -- Students will be evaluated by (5) regularly scheduled tests of 100 points each covering lecture and
laboratory. Comprehensive Final Examination worth 200 points, covering lecture and laboratory.
In addition to the examinations, each student is required to complete a scientific research paper of between 3 and 5
pages and present it to the class. Focus: the description and characteristics of a specific organism of veterinary medical
importance. Topics will be chosen by the student and approved by the instructor. Students will sign-up for
presentation date, presentations during weeks 4-14. The paper shall be in APA format, double-spaced, 1 inch
margins, font size 12 Times New Roman and is worth 100 points. Papers not adhering to APA format will be rejected
for student rewrite and will automatically lose 1 letter grade. Your presentation must have visual aids: powerpoint,
poster, handouts or other demonstration material. References: You must have at least 2 textbooks and at least 1
reputable journal article in addition to other references.
LABORATORY -- Demonstration to the instructor’s satisfaction of laboratory tasks and procedures. Lab sessions will
be worth 10 points each. Students will earn lab points through participation, completion/submission of weekly
laboratory reports and instructor evaluation of weekly laboratory activities (growth/test results). Each student will
complete one Unknown Identification, worth 50 points. There is 1 practical laboratory test covering laboratory
information and techniques, worth 100 points. There are 50 points allotted to unannounced quizzes and/or BlackBoard
(BB) discussions. Field Trips will have 1-page papers, each worth 50 points.
LECTURE POINTS POSSIBLE
LAB PARTICIPATION(30 labs)
LAB UNKNOWNS & Practicals
FIELD TRIP & PAPERS
QUIZZES, BlackBoard
GRAND ROUNDS
TOTAL POINTS POSSIBLE
800
300
300
100
50
50
90% +
80 – 89%
70 – 79%
60 – 69%
< 60%
A
B
C
D
F
1,600
MAKE-UP AND LATE ASSIGNMENTS POLICY
Make-up for lecture examinations must occur within 1 week of the scheduled examination, or with permission of the
Instructor. Make-up examinations may be of a different style, (for example, all essay questions) at the Instructor's
discretion and are taken in the Academic and Campus Services (ACS) office, I-106.
Make-up for missed laboratory sessions are at the Instructor’s discretion. (Some labs simply cannot be duplicated).
Students must arrange with the Instructor to make-up lab activities. Lab reports remain due regardless of whether you
were able to perform the lab or not, due date at the Instructor’s discretion. Unknowns will be performed or student
will receive a “0” score. THERE WILL BE NO MAKE-UP OF LABORATORY PRACTICAL EXAMINATIONS.
Late Assignments require approval by the Instructor. You will automatically lose 1 grade level for all late assignments
(ex. assignment will receive no higher than a grade of "B").
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COMMUNICATION with the Instructor is the key. I emphasize contacting the Instructor as soon as you know of a
potential problem.
GENERAL POLICIES:
1. TCC prohibits smoking or the use of other tobacco products within all College-owned or leased buildings.
2. Eating and/or drinking is not permitted in the classroom, unless specified by your physician and Student Health
Services is apprised.
3. The College student is considered a responsible adult. The student’s enrollment indicates acceptance of those
standards of conduct which appear in the student handbook and activities calendar.
4. Dropping / Withdrawal policy – as specified in the College Catalog. Consult this Semester’s Calendar. Please
meet with instructor(s), Program Coordinator and/or Counseling prior to dropping or withdrawing from any course
in the Veterinary Technology Program.
5. Appropriate dress is required for each course. Consult the instructor about the specific dress code for this course.
6. Students should be on time to class out of respect to other class members and the learning process.
7. Any incidence of cheating or academic dishonesty will result in a grade of zero on that assignment/exam and the
assignment/exam will not be dropped, and incident will be referred to the Dean of Student Services and/or Dean of
Instruction for further action. The second incidence of cheating or academic dishonesty will result in an “F” for
the course.
8. Late assignments / make-ups policies – Late assignments will not be accepted, unless previously arranged with the
instructor. Make-up tests must be arranged in advance with the instructor. In case of emergency absences, the
student must make arrangements with the instructor and Program Coordinator.
ATTENDANCE
Please refer to the General Policies. Attendance will be taken at every lecture and lab. Anticipated absences must be
arranged with the instructor and/or Program Coordinator. Regular class attendance is not only important from the
standpoint of learning, but also is a must in order to be successful in this course. Students are held responsible for all
materials covered during their absence and any changes made in the agenda.
An Excessive Absence notice will be issued after a student misses the equivalent of two class periods and is not
maintaining a “C” average for the class. The Excessive Absence notice will automatically notify the Financial Aid
office and Veterans Services, if applicable. The student may also be subject to Administrative Withdrawal (AW) for
non-attendance.
WITHDRAWAL, AUDIT, AND INCOMPLETE POLICY
To withdraw from the class, the student should initiate an official withdrawal through the counseling office; nonattendance DOES NOT constitute official withdrawal. Failure to withdraw may result in the student receiving a
regular grade of “F” at the end of the semester.
To request a change to Audit (A) or Incomplete (I), the student must be maintaining a passing grade. The student must
initiate the change with the instructor and sign the audit or incomplete agreement. If the Incomplete is due to a
circumstance that prevents the student from being able to sign the form him/herself, the instructor may make other
arrangements.
The last date to withdraw or change to audit for the Fall 2006 semester is Nov. 10, 2006.
READING & WRITING TUTORING SERVICES
The Technology Learning Center’s Reading & Writing Services helps student’s build analytic reading, solid research
and effective writing skills. Additionally, Reading & Writing Services can supplement classroom instruction with
information on MLA, APA, or Chicago Manual styles, Internet research strategies, source evaluation and
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documentation methods, and essay writing techniques. Hours: Monday-Thursday 8:00 am – 8:00pm; Friday 8:00am –
5:00pm; Saturday 8:00am – 1:00pm. (subject to change)
INCLEMENT WEATHER AND CLASS CANCELLATION POLICY
When severe winter weather hits, you may assume that TCC will be open for classes unless it is announced otherwise
on television, radio stations and on the TCC web site. TCC does not automatically close if Tulsa Public Schools close.
If your instructor decides individually to cancel class because of inclement weather or other unexpected reasons, your
instructor will contact you. It is important that your instructor have current contact information so you can be notified
if necessary. Notify the TCC Registrar’s office and your instructor if your contact information changes during the
semester.
PLAGIARISM POLICY
Deliberate plagiarism is claiming, indicating, or implying that the ideas, sentences, or words of another writer are your
own; it includes having another writer do work claimed to be your own, copying the work of another and presenting it
as your own, or following the work of another as a guide to ideas and expression that are then presented as your own.
At the instructor’s discretion, a student guilty of deliberate plagiarism may receive a zero for the assignment
and an “F” in the course.
Accidental plagiarism is the handling of quotations and paraphrases without a deliberate attempt to deceive; it includes
failing to mark the beginning of paraphrases, failing to get away from the language of the original text when
paraphrasing, failing to mark quotations with properly placed quotation marks, and failing to properly identify the
source of a quotation or paraphrase. At the instructor’s discretion, a student whose paper contains accidental
plagiarism may have the opportunity to rewrite the paper with a reduction in grade.
ACADEMIC DISHONESTY OR MISCONDUCT
Academic dishonesty or misconduct is not condoned nor tolerated at campuses within the Tulsa Community College
system. Academic dishonesty is behavior in which a deliberately fraudulent misrepresentation is employed in an
attempt to gain undeserved intellectual credit, either for oneself or for another. Academic misconduct is behavior that
results in intellectual advantage obtained by violating specific standard, but without deliberate intent or use of
fraudulent means. Academic dishonesty or misconduct cases are governed by the Campus Student Rights and
Responsibilities Code (see Student Conduct Handbook).
COMPUTER SERVICES ACCEPTABLE USE
Access to computing resources is a privilege granted to all TCC faculty, staff, and students. Use of TCC computing
resources is limited to purposes related to the College’s mission of education, research, and community service.
Student use of technology is governed by the Computer Services Acceptable Use Statements/Standards found in the
TCC Student Code of Conduct Policy Handbook. Handbooks may be obtained by contacting any Student Activities or
Dean of Student Services office, or Online at www.tulsacc.edu, http://www.tulsacc.edu/page.asp?durki=2984
CLASSROOM ETIQUETTE
All classroom and lab areas are to be cleaned and organized before leaving class. Cell phones are to be turned off
during lab and lecture unless special arrangements are made.
INSTITUTIONAL STATEMENT
Each student is responsible for being aware of the information contained in the TCC Catalog, TCC Student Handbook,
and semester information listed in the Class Schedule.
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LECTURE SCHEDULE
Week
Date
4-14
1
Topic
Reading
Student Presentations – one/two per week
18Aug08
Introduce Syllabi
Introduction to Microorganisms and History
Classification and ID of Microorganisms
Presentations (10 min each)
B: Ch 1, Ch 4 p 112-end
SG: Ch 1
IH: Ch 1
Mc: Ch 8  {Read Mc Ch8 ALL THRU course}
20Aug08
Chemical properties
B: Ch 2
Osmolarity and Transport
B: Ch 3 p 69-73, Ch 6 p 173-4
B: Ch 3; Ch 4 p 112-end
SG:
IH: Ch 3 p 11-21
** READ ** ** READ ** ** READ **
B: Ch1-4, Ch 11, Ch 6 p 174-184, Ch 11 p315-26,
Appdx A
IH: Ch 3-4
Physical Characteristics-Structure and Function
2
25Aug08
3
01Sep08
(Labor Day Monday Sept 1 – NO CLASS)
4
5
6
(Wed)
03Sep08
----Structure and Function (cont’d)
Microscopy, Staining and Classification
B: Ch 3, Ch 4 (all), Ch 6 p 174-184, Ch 11 p315-26,
Appdx C
IH: Ch 2, 3 & 4, Ch 5
08Sep08
EXAM #1 (week 1-3)
Microbial Metabolism
B: Ch 5, Appendix A (pathways), Appendix B
SG:
IH: Ch 4 p 22-27
15Sep08
Microbial Nutrition and Growth
Microbial Genetics
Recombinant DNA and Biotechnology
B: Ch 6 p 165-174, p 184-end, Ch 7, Ch 8
SG:
IH: Ch 5
22Sep08
EXAM # 2 (Weeks 4-5)
Pathogenic Gram + Bacteria
B: Ch 11 p 326-end, Ch 19
SG:
IH: Ch 6,7,8
Pathogenic Gram – Bacteria
B: Ch 11 p 326-end, Ch 20
SG:
IH: Ch 9-17 (9 chapters, 44 pgs)
24Sep08
Page 6
7
29Sep08
Pathogenic Gram – Bacteria
B: Ch 11 p 326-end, Ch 20
SG:
IH: Ch 9-17 (9 chapters, 44 pgs)
8
06Oct08
Mycoplasmas, Rickettsias, Chlamydias,
Spirochetes, Vibrios
B: Ch 21
HO: organisms of VM importance
13Oct08
EXAM # 3 (week 6-8)
Characterization & Classification of Eukaryotes
Pathogenic Fungi and Mycology –Superficial &
Systemic
B: Ch 3 p77-end, Ch 12, Ch 22
SG:
IH: Ch 18 – 26 (9 chapters, 34 pgs)
Mc: Ch 8 (p170-171)
20Oct08
Characterization & Classification of Viruses,
Viriods, and Prions
Pathogenic DNA Viruses
Pathogenic RNA Viruses
B: Ch 13, Ch 24, Ch 25
HO: organisms of VM importance
IH: Ch 27 – 30 (4 chapters, 18 pgs)
Mc: Ch 8 (p171-172)
11
27Oct08
EXAM # 4 (week 9-10)
Controlling Microbial Growth
Sanitation
B: Ch 9, Ch 10
SG:
Mc: Ch 8 (Nosocomial inf to end), Ch 22 p 692-703
12
03Nov08
Controlling Microbial Growth
Sanitation
HO: Dr. Weaver’s Notes
Mc: Ch 8 (Nosocomial inf to end), Ch 22 p 692-703
13
10Nov08
Controlling Microbial Growth – Antibiotic Sensitivity
B: Ch 10
SG:
17Nov08
EXAM # 5 (week 11-13)
Infection and Epidemiology
(Principles of Disease, Epidemiology, Microbial
Pathogenicity)
B: Ch 14, Appdx D
SG:
Mc:
(Wed)
19Nov08
Innate Immunity
Adaptive Immunity
B: Ch 15, Ch 16
SG:
24Nov08
Immunization and Immune Testing
Hypersensitivities, Autoimmune Diseases and
Immune Deficiencies
B: Ch 17, Ch 18
SG:
Mc: Ch 8 (p172-178 Serology-Nosocomial)
9
10
14
15
Page 7
(Wed)
26Nov08
16
01Dec08
Wed Nov 26 – Thanksgiving – No Class
Student-Guided Review
Microorganisms and Animal Disease – selected
topics
17
08Dec08
COMPREHENSIVE FINAL EXAMINATION
Mc: Ch8 (p162-186 including Mycoplasma)
** Lecture & Lab Material **
Reading Abbreviations:
xxxxx
xxxxxxxxxxxxx
xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
Extra
unscheduled
Field Trip - Wastewater Treatment Plant
Applied and Environmental Microbiology
Field Trip – Microbiology Laboratory
Paper Presentations (10 min each)
B: Bauman, Microbiology with Dz by Tax. (textbook)
SG: Study Guide
IH: Ikram and Hill, Microbiology for Vet Techs
Mc: McCurnin, Clinical Textbook for Vet Techs
xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
B: Ch 26
TBD
Page 8
LABORATORY SCHEDULE
Week
Date
Topic
Laboratory Safety
Lab Orientation, Syllabus and Lab Manual “cruise”
Reading
L: Introduction p 1-5; HO’s
DVD: Safety In the Microbiology Laboratory (14 min)
L: Introduction p 5-6; Sect. 1 Fundamental Skills p 7
Experimental Design
L: p 7, Ex 1-5
HO: Sampling
Hx: Ch 4 p 105-112
Sampling
1a
18Aug08
3-1 Light Microscope
Orientation, Proper cleaning, Proper storage
Assign Lab Drawers and Smock Storage
Video: Use and Care of the Microscope (23 min)
L: Ex 3-1 (prepared slides- parasites & bacteria)
1-1 Hand Wash Education (dry lab)
1-2 Media Preparation (dry lab)
Core Technique: Measures and Weighing,
Reading the Meniscus
L: p 7, Ex 1-1; 1-2, Apdx B
IH: Ch2 p 5-6
HO: Core techniques
Collaboration and Teamwork Discussion
2-1 Ubiquity of Microorganisms & EnvironmentalSampling
1b
20Aug08
******
Thursday
1-3 Common Aseptic Transfers and Inoculations
CT: Stirring broth tubes (vortex mixer and by
hand), Aerosol avoidance
READ: 2-1, 1-3
L: 2-1
IH: Ch5 p 29-39
L: 1-3
Hx: Ch 4 Diagnostic Microbiology, p128-32, 138-41
READ: 2-1, 1-3 – Collaborate w/ colleagues, Share data
L: p47, 2-2, 2-3, 2-4;
2a
25Aug08
2b
27Aug08
3a
1Sep08
CT: Positive and Negative Controls
2-2 Colony Morphology (use 2-1 plates)
2-3 Growth Patterns on Slants
}
2-4 Growth Patterns on Broth
} (use 1-3 tubes)
3-3 Examination of Eukaryotes (pond H20)
READ: (previous exercises)
p119, 3-3, 3-10
** 3-10 Omit Hanging Drop section
HO’s
Hx: Ch 4 Diagnostic Microbiology
{pond H2O, Bacillus, E.coli, Serratia)
Discuss: Dr. W’s “Trilogy” morphology/motility/Gram stain
L: 3-4, 3-5
** Ex 3-5: Make 2 slides – 1 heavy inoculum, 1 thin
Hx: Ch 4 Diagnostic Microbiology
3-10 Wet Mounts and MOTILITY
3-4 Simple Stains
3-5 Negative Stains
LABOR DAY
READ READ READ READ READ READ READ READ
Page 9
3b
3Sep08
4a
8Sep08
4b
10Sep08
1-4 Streak Plate Methods of Isolation
(Isolation by Dilution Technique)
Discuss Data Sheets and Lab Reports, Progress to date
READ: 1-4 plates
L: 1-4
3-6 Gram Stain
Questions???
L: 3-6 (Hint: Colony morphology 2-2)
Hx: Ch 4 Diagnostic Microbiology, p105-214
IH: Gram stain
Nutrition:
2-5 Evaluation of Media (nutritional requirements)
5-20 BAP vs TSA
(Blood Agar Plate vs Tryptic Soy Agar)
L: p 47, 2-5, 5-20
HO – BAP (blood agar plate), TSA (tryptic soy agar),
NA (nutrient agar plate)
READ: 2-5, 5-20, Gram Stain – 1 from each plate
5a
5b
******
L: p 225, 5-1, 2-6
17Sep08
Metabolic Pathways:
Cellular Respiration vs Fermentation
5-1 Oxidation / Fermentation Test (O/F Tubes)
2-6 Fluid Thioglycollate Medium
READ: 5-1, 2-6
Metabolic Pathways:
Cellular Respiration vs Fermentation
5-4 Catalase Test
5-5 Oxidase Test
Carbohydrate Catabolism
5-2 Phenol Red Broths
Thursday
READ: 5-2
** Gram stain Turn In
** Practice Motility and Gram stains – Use PR broths; Turn in
student slides to Dr.W & Katherine properly ID’d
Metabolic Pathways:
Protein catabolism
5-3 MRVP Methyl Red / Voges-Proskauer
5-12 Urease Test
5-14 Gelatinase Test (Gelatin Hydrolysis/catabolism)
5-8 Decarboxlylation (amino acid catabolism)
L: 5-3, 5-12, 5-14,5-8
Hx: Ch 4 Diagnostic Microbiology, p105-214
15Sep08
6a
22Sep08
******
Tuesday
?? Questions about Lab Reports ??
L: 5-4, 5-5; 5-2, 5-11
Hx: Ch 4 Diagnostic Microbiology, p105-214
IH: Catalase & Oxidase tests, Carb metabolism
(MR/VP requires 48 hrs, others exercises may require 48hr +
incubation time; decarbox & gelatin 5-7 days)
** Lots to inoculate !! ORGANIZE and PLAN !!
** Practice Gram stains – Use Urease tubes
READ: 5-12 Urease
READ: 5-3 MRVP
CHO and Protein Metabolism, final pH, Energy Pathways and
inducible pathways, Production of proteins (enzymes) and
genetic info need to produce them
6b
24Sep08
7a
29Sep08
QUIZ next Monday – up to this point including today’s
Basic techniques and stains, Identification and
explanation of test results
Discuss ?’s to date – Student Guided Review
Basic techniques and stains, Identification & explanation of test
results (up to this point including week 6 labs)
*** LAB PRACTICAL ***
Page 10
******
Tuesday
READ: 5-14 Gelatinase, 5-8 Decarboxylations
** Lots to inoculate !! ORGANIZE and PLAN !!
Discuss Differential vs Selective
7b
******
1Oct08
Thursday
L: p 197, 4-1, 4-2, 4-4, 4-6
Each student will streak all 4 plates with
Differential Media: Read and review p 197, 4-3, 4-5 (omit
procedures, any ??’s)
(Some of these media have multiple biochemical reactions
occurring—be sure to record them ALL.)
Differentiation of Bacteria:
Selective vs Differential Media
4-1 Mannitol Salt Agar (MSA)
4-2 Phenylethyl Alcohol Agar (PEA)
4-4 Eosin Methylene Blue Agar (EMB)
4-6 MacConkey Agar
(Some of these media have multiple biochemical reactions
occurring—be sure to record them ALL.)
READ: (previous labs) Remember TEAMWORK !!
READ: (previous labs)
L: 5-21, 5-10, 5-19
review 5-20 Blood Agar Plate (review hemolysis);
4-1 Mannitol Salt; 4-2 PEA
L: p 455, 9-2 & 9-6 Demonstration, HO
Bergey’s Group 17
Staphylococcus, Streptococcus (Lancefield groups) & S.bovis,
S.pyogenes, S.equi, Enterococcus, Ruminococcus
Group 18 Clostridium, Bacillus
Group 19 Erysipelothrix, Listeria
Group 20 Actinomyces, Bifidoacterium, Corynebacterium
Group 22 Nocardia, Rhodococcus (R.equi-formerly Coryne)
Group 21 Mycobacteria
Gram Positive Cocci and Rods:
5-21 Coagulase Test
5-10 Bile Esculin Test (dry lab)
5-19 Bacitracin Disc (on BAP)
9-2 Identification of G(+) Cocci
CAMP Test
PYR Test (dry lab)
Optochin Test
9-6 ID Streptococci by Latex Agglutination (dry lab)
8a
6Oct08
READ: (previous labs)
8b
8Oct08
13Oct08
9a
******
Tuesday
L: 3-8, 5-4
IH: structural stains
Structural stains:
3-8 Capsule Stain
5-4 Catalase test
** Gram Stains
< Continue G(+) Bergey’s Groups >
L: all applicable exercises, “Trilogy”, API HO’s, Bergey’s
Manual and other references, (oxidase, catalase, capsule stain)
< Continue G(+) Bergey’s Groups >
Gram Positive Cocci:
API Staph
API Strept
DDx: Staph vs Strept
DDx: Staph spp and Strept spp
** API Strept – read some tests in 4 hours **
Hosp II lab – obtain gingival pocket inoculums & set up
anaerobic cultures
(or, you may use your own gingiva for next lab)
Page 11
Gram Positive Rods (and Cocci):
Endospore vs. Non-Endospore Forming Rods
L: 3-9, 2-7
Group 18: G+ endospore rods: Bacillus, Clostridium
Group 19: G+ non-endo rods: Erysipelothrix, Lactobacillus, Listeria
Group 20: Pleomorphic non-endo rods:
Actinomyces, Bifidobacterium, Corynebacterium,
Propionibacterium
Group 22: Pleomorphic non-endo rods: Rhodococcus (formerly
Corynebacterium)
L: 3-7
Bergey Group 21-22: Mycobacterium, Nocardia, Actinomyces
Other Acid-Fast organisms: Rhodococcus +/- weak positive,
Protozoan oocysts – Cryptosporidium, Isospora
Others:
Dermatophilus, Streptomyces
3-9 Endospore stain
2-7 Anaerobic Environment (& 2-6)
(oral gingival pocket inoculum)
9b
15Oct08
Acid Fast Microorganisms
3-7 Acid Fast Stain
10a
10b
20Oct08
22Oct08
(“Gram+” because they don’t decolorize well)
Gram Negatives: part 1
Enterobacteriaceae
5-18 Kligler’s Iron Agar (&Triple Sugar Iron Agar (TSI)
5-6 Nitrate Reduction
9-1 IMViC tests and Enterobacteriaceae ID
(IMViC is dry lab)
L: p455 5-18, 5-6, 9-1 (These media have multiple biochemical
reactions occurring—be sure to record them ALL.)
******
Thursday
READ: 5-18 Kligler’s, 5-6 NO3
** Schedule your time for reading and recording !!
Thursday
ACR Team –collect 5 fecal samples (Ca, Fe, Lg an, lab an)
Katherine: Thioglycollate and TSB inoculation
Place properly ID’d samples in lab for Katherine
******
Friday
Katherine: Streak plates for isolation MacConkey and BAP
Inoculum = TSB (mix the tube before sampling)
******
** prepare for Monday’s lab
L: 5-5, 9-4, 9-5
** Each student will inoculate 1 Rapid ID test with known
bacterium
** Each table will inoculate 1 Rapid ID test with your choice
from your poop plate
(think about which Rapid ID test you will use & why, based upon
“Trilogy”, oxidase tests – must be approved by Dr. W.)
DUE DATE: __________________
READ: (previous labs) + poo poo platters
Rapid Identification Systems
5-5 Oxidase test on selected bacteria (see Dr. W)
9-4 API 20E
9-5 Enterotube
Bergey’s Group 5 (Subgroup 1-important genera):Escherichia,
Enterobacter, Klebsiella, Salmonella, Shigella, Proteus, Serratia,
Yersinia
11a
27Oct08
** must read Rapid ID’s within 24 hours **
(SAVE poop plates for next lab)
Page 12
READ Rapid ID tests
******
Tuesday
Schedule enough time to add reagents, reaction times for
tests and to record your results
Optional testing for Enterobacteriaceae; Others available – see
Dr. W to help with differentiation and ID
ONPG Test , XLD agar, SS Agar
READ: (previous labs) + poo poo platters
L: In-house OxiFerm tubes (all other pertinent labs and
references), HO
Gram Negatives, part 2:
Other than Enterobacteriaceae
ID procedures: “Trilogy”, oxidase, catalase, TSB (O2 req’s),
MacConkey, BAP, O/F tubes
Bergey’s Group 5: (Subgroups 2,3)
Rods straight/curved: Aeromonas, Plesiomonas, Vibrio
Pleomorphic: Actinobacillus, Pasteurella-Burkholderia,
Mannheimia-Hemophilus
Bergey’s Group 4 – Aerobic and Microaerophilic Gram(-):
Rods: Pseudomonas, Francisella, Rochalimaea-Bartonella,
Bacteroides
Coccobacilli or pleomorphic: Bordetella, Brucella, Moraxella,
Branhamella, Taylorella, (Neisseria)
Others: Chlamydophila (aka Chlamydia), Porphyromonas
Curved/Helical: Campylobacter, Helicobacter
Spirochetes: Leptospira, Serpulina-Treponema, Borrelia
5-5 Oxidase test on selected bacteria (see Dr. W)
9-4 API 20E
In-house Lab: OxiFerm tubes
11b
******
12a
******
29Oct08
Thursday
3Nov08
Tuesday
READ Rapid ID tests
(schedule enough time to add reagents, reaction times for tests
and to record your results, due Oct 23)
7-2 Antimicrobial Susceptibility Test (Kirby-Bauer)
L: 7-2
Read 7-2, measure zones w/in 24 hours (mm’s)
(Schedule enough time to record zones for all discs)
L: Read 7-5, 7-6 and textbook Ch26 BEFORE trip
(not procedures)
Field Trip – Water Treatment Plant
12b
5Nov08
Meet at VTC and carpool, special arrangements for those living
close to Plant – see Dr. W
** 1 page paper due MON Nov 10 **
Page 13
L: p 455, all applicable exercises, Lab section 9, Bergey’s
Manual and other Keys
You must decide which Rapid ID test you will use, based upon
“Trilogy”. All oxidase tests – must be approved by Dr. W.
Unknown # 1 with Antibiotic Susceptibility
13a
10Nov08
13b
12Nov08
Lab Report: 9-1,2,3 + Rapid ID sheet + HO (explanation of
tests and support of your ID) + Report of Susceptibility
due
Nov 17
(schedule enough time to add reagents, reaction times for tests
and to record your results, due Nov 17)
L: 2-6, 2-7, 2-8--HO
READ Rapid ID tests
Control of Growth:
2-6 & 2-7 Oxygen as Growth Control (dry lab discussion)
2-8 Effect of Temperature on Microorg’s
14a
17Nov08
Control of Growth:
2-9 Effect of pH on Microorg’s
2-10 Effect of Osmotic Pressure on Microorg’s
2-11 Lethal Effect of UV Light on Microorg’s
L: 2-9, 2-10, 2-11, 2-12
Tables 1 & 2 Ex 2-9 } Collaboration: Share your results
Tables 3 & 4 Ex 2-10 }
with ALL classmates – HINT: for
Tables 5 & 6 Ex 2-11 }
Final Exam !!
2-12 Effectiveness of Hand Washing
Use-Dilution Test (dry lab discussion)
(inoculum = rub your face, nose, forehead with your
inoculating fingertips)
L: 2-12 (ALL Tables perform)
HO: USE DILUTION Lab
14b
19Nov08
2-13 Steam Sterilization
******
Tuesday
READ: (previous labs)
2-13 Retrieve and incubate ampules
24Nov08
15b
26Nov08
L: 2-13 (demonstration-place inside surg pack-autoclave and
use pack Tues—incubate Tuesday )
L: 3-3; HO (fungal ID keys)
Yeast: Malessezia, Candida, Saccharomyces
Mold: Penicillium, Trichophyton, Microsporum, Aspergillus,
Mucor, Rhizopus, Fusarium
L: Appendix C, D + HO
Mycology:
3-3 The Fungi-Common Yeast and Molds
CT: Pipetting
15a
** Bring Soap or Sanitizer of your choice for next lab Ex 2-12 **
** Bring liquid disinfectant & manufacturer’s directions for
dilution -- for next lab **
Thanksgiving Vacation
(prehension, mastication, deglutition, digestion)
Page 14
** Lab first, then Lecture, then Lab to read results **
16a
1Dec08
*****
Tuesday
16b
3Dec08
17a
8Dec08
Molecular Biology: Electrophoresis
HO: Electrophoresis, OSU “Footlocker” Ex - Electrophoresis
Epidemiology
7-3 MMWR Report
7-4 Epidemic Simulation
L: 7-3, 7-4, CDC Website—MMWR reporting, OK State Dept of
Health Report
Review: CMT Californial Mastitis Test
HO: CMT
Field Trip - Dairy or Diagnostic Lab (TBA)
California Mastitis test
HO: California Mastitis test
Check in Lab Drawers
Final Lab Cleaning
Reading Abbreviations:
L: Leboffe & Pierce, Microbiology Laboratory Theory , and
Application, Brief Edition (lab book)
IH: Ikram, Hill Microbiology for Vet Techs
Hx: Hendrix, Laboratory Procedures for Vet Techs
HO = Hand Out (“stuff” from Dr W)
CT = Core Technique
xxxx
xxxxxxxx
Extra
Labs
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Quantitative Techniques:
6-1 Standard Plate Count
6-2 Urine Culture
6-3 Direct Count
LAB FINAL
3-2 Calibration of the Ocular Microscope
3-12 Morphologic Unknown
3-12 and perform ID stains
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L: 6-1, 6-2, 6-3, HO-CMT Mastitis & Milk
Water-borne Illness (coliforms)
Food-borne illness (Listeria, coliforms, Staph, Strept)
Basic techniques, Gram Stain, Selective & Differential media,
Interpretation of test results, Characteristics of major organisms
(Ex. E.coli, Pseudomonas, Clostridium, etc)
5-11 Starch Hydrolysis
Page 15