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Evaluation of piperlongumine as an anti-cancer drug in oral cancer Speaker : Geng-Hung Liu Advisor : Hau-Ren Chen Date: 2012.5.18 Abstract Oral cancer is one of the most prevalence cancers and is listed as the 6th leading cancer worldwide. In South and Southeast Asia regions, oral cancer was the third leading cancer among all cancers. In Taiwan and India, betel nut is the major risk factor for oral cancer. Unfortunately, there is no proper auxiliary drug for chemotherapy effectively so far. Piperlongumine (PL) is a natural product of the Long pepper (Piper longum), which is a pepper plant found in southern India and Southeast Asia. Recent study discovers that PL is a novel compound and blocks the response to oxidative stress selectively in cancer cells, but spares in normal cells, suggesting that this small molecule probably has the potential to be an anticancer drug in the future. In this study, I first treated two oral squamous cancer cell lines (OC2 and OCSL) with PL and found that PL could suppress the growth of oral cancer cells from MTT assay. To investigate the possible mechanisms for the suppression of cell growth, experiments were performed to examine cell cycle arrest, apoptosis, senescence and autophagy. From preliminary results, I found that PL might induce cell cycle arrest at G0/G1 phase and also cause apoptosis from cytometry analysis by PI single staining or Annexin V/PI double staining, respectively. In addition, protein level of PARP increased in a time-dependent manner after treatment with 20 μM PL. Moreover, OC2 induced senescence significantly in comparison with OCSL after 24 hour treatment. However, the autophagy marker LC3 could not be activated after PL treatment by western blots analysis. In the future, I will test the effect of anti-oral cancer drugs, by co-treatment 5-Fu/cisplantin and PL to investigate whether the combined treatments has the synergistic effect on growth suppression in both oral cancer cell lines. --------------------------------------------------------------------------------------------Evaluation of Piperlongumine as an anti-cancer drug in oral cancer Speaker: Geng-Hung Liu Code: Comments: Score: