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Pathways assessment: Entry Assessment for Exotic Viral Pathogens of Swine Lisa A. Ferguson USDA, APHIS, Veterinary Services NIAA, Omaha April 2014 Objectives • Identify and describe pathways by which exotic viral pathogens of swine may enter the US • Estimate the likelihood that each identified pathway may introduce exotic viral pathogens of swine into the US NO Virus in region YES no Infected or contaminated NO no product selected for export YES Virus survives process prior to export YES NO Virus survives transport to US YES Product contaminated post-process NO Virus survives import mitigation at border YES no no NO no ENTRY Next steps • Pathways with non-negligible likelihood of introduction - estimate likelihood of exposure • Pathways with non-negligible likelihood of exposure – evaluate consequences • Pathways where consequences, overall risk of introduction, and overall risk of exposure are non-negligible – identify potential mitigation measures Risk estimation terms Term Definition Negligible So rare that it doesn’t merit consideration Low Rare but does occur Medium Occurs regularly High Occurs very often Assumptions • One or more exotic viral pathogens are present • Infected animals are selected for slaughter and/or rendering • Current regulations and mitigation procedures effectively enforced Representative viruses • Stand in for both single- and double-stranded RNA and DNA virus • Encompass a range of resistance to inactivation – Classical swine fever – Foot and mouth disease – Pseudorabies Pathway groups • Airborne • Inanimate articles serve as fomites – – – – – Animal tissues or fluid and their products Conveyances and containers Equipments Food and feed Garbage • Live animals that may serve as vectors or fomites – – – – Livestock/germplasm Humans Microorganisms or arthropod vectors Other live animals Feed ingredient origin • Animal (rendered products, marine, dairy, etc..) • Plant (forage, grain, by-products, fats/oils, vitamins, etc…) • Microbial culture (amino acids, minerals, vitamins, etc…) • Mined material (anticaking, colorants, minerals) • Synthetic (amino acids, antioxidants, flavors, minerals, vitamins, etc…) Rendered animal proteins and fats, marine by-products • Includes blood products – none imported • Rendering process inactivates viruses • Post-processing contamination unlikely; transport times and conditions not conducive to virus survival • Negligible risk of entry Milk and milk derivatives • Small amount of imports • Treatment sufficient to inactivate FMD • Post-processing contamination unlikely; transport times and conditions not conducive to virus survival • Negligible risk of entry Animal manure • Virus could be present • Not allowed entry for purpose of feeding • Negligible risk of entry Plants and plant products • Imports – Canada, Argentina, Mexico • Potential for contamination if contact with infected animals/excretions/fomites • ? Virus survival time • Negligible risk of entry if pelleted/treated; low risk of entry if unprocessed Microbial culture • Amino acids (L-carnitine, lysine, etc..), probiotics, enzymes (phytase), vitamin (B2, B12), etc. • Contamination of culture (bacteria, fungi, bacteriophages) considered production failure; GMP control • Recovery of product – sterilization, centrifugation, filtration, crystallization, etc… not conducive to virus survival • Negligible risk of entry Chemical synthesis/mining • Amino acid (methionine), minerals, vitamins (A, B1, B3 or B5, B6, B7, B9, D, K; choline), etc.. • Viruses can’t propagate without living animal cells; contamination unlikely to survive manufacturing process • Post-processing contamination unlikely; transport times and conditions not conducive to virus survival • Negligible risk of entry • Questions?