Download Dr. Ajit Chande - IISER Biological Science

Dr. Ajit Chande
Joining in May, 2017 as Assistant Professor, Biological Sciences, IISER Bhopal
Postdoctoral Fellow, Laboratory of Virus-Cell Interaction
Centre for Integrative Biology (CiBIO), University of Trento
Via Sommarive-9, Povo, Trento, Italy-38123. Cell phone: +39-389 162 9852;
E-mail: [email protected], [email protected]; Skype: ajit.chande
EDUCATION
Ph.D. (2012-Life Science), Homi Bhabha National Institute (HBNI), Mumbai, India.
[Place of research work: Virology lab, ACTREC, Tata Memorial Centre, Kharghar, Navi Mumbai]
M.Sc. (2006-Biotechnology), Amravati University, India.
B.Sc. (2004-Biology and Chemistry), Amravati University, India.
RESEARCH INTERESTS
Virus biology, restriction factors, innate immunity, CRISPER/Cas9 genome engineering,
molecular anti- viral/-tumoral strategy designing.
TECHNICAL EXPERTISE
Pathogenic microbes handling, propagation and maintenance in BSL-3 facility; functional
proteomics, molecular biology techniques;CRISPR/Cas9 precision genome editing; Highcontent screening, high throughput assay development, molecular manipulation of viral,
bacterial, and mammalian cells including cell type specific gene delivery; LCM and
conventional microscopy; animal experimentation, Click-chemistry for functional proteomics,
live cell imaging and post-translational modifications; cell-selective metabolic labeling in
complex cellular mixtures.
RESEARCH EXPERIENCE
Postdoctoral research (July 2014-till date)
Laboratory of Virus-Cell Interaction, CIBIO, University of Trento
Mentor: Prof. Massimo Pizzato, Group Leader Viral Pathogenesis Program
In the molecular arms race between viruses and their host cells, each side employs multiple
strategies to deal with the other. Whereas the host has sophisticated antiviral signaling
programs to combat viral infection, viruses evolved their own proteins to subvert these host
defences. Studies towards resolving these interactions and pathogen’s evasion tactics,
however, potentially may translate into alternate therapeutic strategies to reduce disease
burden. We have recently discovered a cellular factor SERINC5 that potently inhibits HIV-1 and
other retroviruses. This host-factor has a broad spectrum activity and we have now identified
three different retroviruses have developed countermeasures to inhibit this host restriction.
We are currently exploring the mechanisms of retrovirus restriction exercised by SERINC5 and
investigating the existence of further cross-species anti-retroviral barriers.
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PUBLICATIONS
1. Sood C, Marin M, Chande A, Pizzato M, Melikyan GB. SERINC5 Inhibits HIV-1 Fusion Pore Formation
by Promoting Functional Inactivation of Envelope Glycoproteins. Journal of Biological Chemistry
2017; ahead of print.
2. Chande A, Cucurullo E, Ziglio S, Carpenter S, Pizzato M. S2 from Equine infectious anemia virus is an
infectivity factor which counteracts the retroviral inhibitors SERINC5 and SERINC3. Proceedings of
the National Academy of Sciences, USA 2016; 113(46):13197-13202.
3. Rosa A*, Chande A*, Ziglio S*, De Sanctis V, Bertorelli R, Goh S L, McCauley SM, Nowolsielska A,
Antonarakis SE, Luban J, Santoni FA and Pizzato M. HIV-1 Nef promotes infection by excluding
SERINC5 from virion incorporation. Nature 2015; 526:212-7. (*contributed equally)
[Article Preview in- Nature 526, 202–203 (2015) & -Nature Reviews Microbiology 13, 660–661 (2015); Recommended by F1000 as a must
read article]
4. Chande AG#, Siddiqui Z, Midha MK, Ravichandran R, Rao KVS. Selective enrichment of mycobacterial
proteins from infected host macrophages. Scientific Reports 2015; 5:13430. (#corresponding author)
5. Khan TH, Srivastava N, Srivastava A, Sareen A, Mathur RK, Chande AG, Musti KV, Roy S,
Mukhopadhyaya R, Saha B. SHP-1 Plays a Crucial Role in CD40 Signaling Reciprocity. The Journal of
Immunology 2014;193:3644-53.
6. Ukil A, Gupta P, Giri J, Srivastav S, Chande AG, Mukhopadhyaya R, Das PK. Leishmania donovani
negatively regulates TLR4 mediated host response by preventing ubiquitination dependent
degradation of TRAF3. The FASEB Journal 2014;28:1756-68.
7. Dhamne H*, Chande AG* and Mukhopadhyaya R. Lentiviral vector platform for improved
erythropoietin expression concomitant with shRNA mediated host cell elastase down regulation.
Plasmid 2014;71:1-7 (*contributed equally).
8. Raina S, Chande AG, Baba M and Mukhopadhyaya R. A reporter based single step assay for
evaluation of inhibitors targeting HIV-1 Rev-RRE interaction. VirusDisease 2014;25:101-6.
9. Goel P, Tailor P, Chande AG, Basu A, Mukhopadhyaya R. An infectious HHV-6B isolate from a healthy
adult with chromosomally integrated virus and a reporter based relative viral titer assay. Virus
Research. 2013;173:280-5.
10. Chande AG, Raina S, Dhamne H, Kamat RH and Mukhopadhyaya R. Multiple platforms of a HIV-2
derived lentiviral vector for expanded utility. Plasmid 2013;69:90-95.
11. Sarma U, Sareen A, Maiti M, Kamat V, Sudan R, Pahari S, Srivastava N, Roy S, Sinha S, Ghosh I,
Chande AG, Mukhopadhyaya R, Saha B. Modeling and experimental analyses reveals signaling
plasticity in a bi-modular assembly of CD40 receptor activated kinases. PLoS One 2012; 7(7):e39898.
12. Srivastav S, Kar S, Chande AG, Mukhopadhyaya R, Das PK. Leishmania donovani Exploits Host
Deubiquitinating Enzyme A20, a Negative Regulator of TLR Signaling, To Subvert Host Immune
Response. The Journal of Immunology 2012;189:924-34.
13. Chande AG, Baba M, Mukhopadhyaya R. A Single Step Assay for Rapid Evaluation of Inhibitors
Targeting HIV Type 1 Tat-Mediated Long Terminal Repeat Transactivation. AIDS Research and
Human Retroviruses 2012;28:902-6.
14. Basu Ball W, Kar S, Mukherjee M, Chande AG, Mukhopadhyaya R, Das PK. Uncoupling protein 2
negatively regulates mitochondrial reactive oxygen species generation and induces phosphatasemediated anti-inflammatory response in experimental visceral leishmaniasis. The Journal of
Immunology 2011;187:1322-32.
15. Dutt A, Ramos AH, Hammerman PS, Mermel C, Cho J, Sharifnia T, Chande A, Tanaka KE, Stransky N,
Greulich H, Gray NS, Meyerson M. Inhibitor-sensitive FGFR1 amplification in human non-small cell
lung cancer. PLoS One. 2011;6(6):e20351.
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16. Chande A, Kövics GJ, Sandhu SS, Rai MK. Morphological and genetic differentiation among four
pigment producing Indian species of Phoma (Saccardo, 1899). Indian Journal of Microbiology 2010
;50:110-16.
CONFERENCES/ ABSTRACTS/PRESENTATIONS
1. “A novel lentiviral vector for enhanced biodistribution of gene products” in the 2 nd Molecular
Virology Meeting, IISc, Bangalore India (2011). [Poster presentation]
2. “A novel, sensitive assay to detect and quantify retrovirus fusion to target cells” in the Retroviruses,
Cold Spring Harbour Laboratory, USA (2015). [Poster presentation]
3. “Identification of a host factor that inhibits retrovirion infectivity and is counteracted by Nef and
glycoGag” in the Retroviruses, Cold Spring Harbour Laboratory, USA (2015).
4. “Evaluation of a lentiviral based fusion assay as a novel neutralisation assay for Ebola virus” in the
Annual meeting of the Microbiology Society, in Liverpool-UK (2016).
5. “SERINC5: evidence for an ancestral antiretroviral activity and molecular determinants required for
HIV-1 inhibition” in the Retroviruses, Cold Spring Harbour Laboratory, USA (2016). [Oral
presentation]
6. “The S2 accessory protein of EIAV is an infectivity factor which counteracts SERINC5” in the
Retroviruses, Cold Spring Harbour Laboratory, USA (2016). [Poster presentation]
HONORS & OTHER SCIENTIFIC RECOGNITIONS
• University first rank medal in M.Sc. (Biotechnology) by Amravati University.
• Junior Research Fellowship, ACTREC, Government of India (Aug 2006-Oct 2008)
• Senior Research Fellowship, ACTREC, Government of India (Nov 2008-Dec 2011)
• Best poster award: in the 2nd Molecular Virology Meeting, IISc, Bangalore, India (2011).
• Research Scientist at ICGEB-India (from Jan 2012-June 2014).
• Invited talk at Biocon-Bangalore: “Lentiviral vectors for transgenesis and genome editing
through CRISPR/Cas9” (Oct 13, 2015).
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