Download Annex A M. PHIL PROGRAMME IN MOLECULAR”BIOLOGY

Annex A
M. PHIL PROGRAMME
IN MOLECULAR”BIOLOGY, VIROLOGY and IMMUNOLOGY
NATIONAL CENTRE OF EXCELLENCE IN VIROLOGY AND IMMUNOLOGY
For M. Phil Degree, 24 credit hours course work +06 credit hours for one year of original
research work and for Ph.D degree additional 18 credit hours course work + 30 credit hours of
original research work will be required.
M Phil. Semester-I
Course No.
Course Title
Credit Hours
Status
MVI-800
Gen & Mol. Virology
3
Core
MVI-810
Cell and Mol. Biology
3
Core
MVI-930
Biosafety and Bioethics
3
Core
MVI-830
Gen & Mol. Immunology
3
Core
Semester –I Total Credits
12
M.Phil. Semester-II
Course No. Course Title
Credit Hours
Status
MVI-804
Bacteriophage Biology
3
Core
MVI-860
Viral Pathology
3
Core
MVI-850
Signal Transduction
3
Core
MVI-901
Bioinformatics
3
Elective
MVI-xxx
Elective – II
3
Elective
Semester – II Total Credits
12
M Phil. Thesis Research
6
Total Credits
30
MVI-899
List of Elective_II. Courses
MVI-810
Recombinant DNA techniques
MVI-820
Microbiology
MVI-834
Immune Pharmacology
MVI-880
Molecular & General Toxicology
MVI-920
Lab. Diagnostic Techniques
(3 Credit Hours)
(3 Credit Hours)
(3 Credit Hours)
(3 Credit Hours)
(3 Credit Hours)
1
Compulsory
Annex B
PH D COURSE WORK
IN MOLECULAR”BIOLOGY, VIROLOGY and IMMUNOLOGY
NATIONAL CENTRE OF EXCELLENCE IN VIROLOGY AND IMMUNOLOGY
PhD. Semester-I
Course No.
Course Title
Credit Hours
Status
MVI-810
Adv. in Mol. Biology
3
Core
MVI-801
Adv. in Virology
3
Core
MVI-xxx
Elective – I
3
Elective
Scientific writing and presentation skills
Total Credits
PhD. Semester-II
Course No.
Non credit course
9
Course Title
Credit Hours
Status
MVI-851
Medical Genetics
3
Core
MVI-870
Vaccinology
3
Core
MVI-xxx
Elective – II
3
Elective
Total Credits
9
PhD Thesis Project:
30
Total Credits
(excluding MPhil Credits)
48
MVI-999
Elective Courses:
MVI-840
Trends in Pharmacology
MVI-852
Immunogenetics
MVI-861
Molecular Mechanisms of Disease
MVI-910
Stem Cell Studies
MVI-940
Experimental Therapeutics
MVI-950
Plant viruses and diseases
2
Annex C
NCVI Core Curriculum
MPhil/PhD program
Following is the list of course that will be initially offered in NCVI. With the induction of more
students and faculty, additional course will be offered and some will be modified. The changes will
be made periodically by the academic body of NCVI and sent to Academic and Examination (A &
E) body of NUST for final approval.
Major Courses CODE List
Course Title
Serial No Course Code
1
MVI-80
Virology
2
MVI-81
Molecular and Cell Biology
3
MVI-82
Microbiology
4
MVI-83
Immunology/Immune disorders
5
MVI-84
Pharmacology (Viral, Immune etc)
6
MVI-85
Medical Genetics/ Targeted Gene Therapy
7
MVI-86
Viral Pathology
8
MVI-87
Vaccinology
9
MVI-88
Metabolism/Toxicology
10
MVI-89
Biotechnology/Fermentation
11
MVI-90
Bioinformatics
12
MVI-91
Stem Cell Studies
13
MVI-92
Lab Diagnostic Techniques/Instrumentation
14
MVI-93
Biosafety/Bioethics
15
MVI-94
Experimental Therapeutics
16
MVI-95
Plant viruses and diseases
Each course has further Subdivisions as listed below.
3
.
Serial
No
MVI-80
1
Virology
MVI-81
2
Molecular Biology/Cell
Biology
MVI-82
3
Microbiology
MVI-83
4
Immunology/Immune
Disorders
MVI-84
5
Pharmacology (Viral,
Immune etc)
MVI-85
6
Course Title
Course Code
Medical Genetics/ Targeted
Gene Therapy
CODE
800
801
802
803
804
805
CODE
810
811
812
813
814
815
CODE
820
821
822
823
824
825
CODE
830
831
832
833
834
835
CODE
840
841
842
843
844
845
CODE
850
851
852
4
.
CONTENTS
GENERAL VIROLOGY
Basic Concepts in Virology
ADVANCES IN VIROLOGY
MECHANISTIC VIROLOGY
Bacteriophage Biology
CONTENTS
MOLECULAR BIOLOGY
CELL BIOLOGY-I
CELL BIOLOGY -II
GENOMICS AND PROTEOMICS
CONTENTS
MOLECULAR MICROBIOLOGY_1
MOLECULAR MICROBIOLOGY_2
CONTENTS
CLINICAL IMMUNOLOGY 1
CLINICAL IMMUNOLOGY 2
ANTIVIRAL IMMUNOLOGY
CONTENTS
PHARAMACOLOGY
CONTENTS
GENERAL GENETICS
MEDICAL GENETICS
IMMUNOGENETICS
MVI-86
7
Microbial Pathology
MVI-87
8
Vaccinology
MVI-88
9
Metabolism/Toxicology
MVI-89
10
Biotechnology/Fermentation
MVI-90
11
Bioinformatics
MVI-91
12
Stem Cell Studies
853
854
855
CODE
860
861
862
863
864
865
CODE
870
871
872
873
874
875
CODE
880
881
882
883
884
885
CODE
890
891
892
893
894
895
CODE
900
901
902
903
904
905
CODE
910
911
912
913
5
GENETHERAPY
CONTENTS
Viral Pathology
Molecular Mechanisms of Disease
CONTENTS
VACCINOLIOGY
CONTENTS
MOLECULAR TOXICOLOGY
CONTENTS
BIOTECHNOLOGY
CONTENTS
BIOSTATISTICS
BIOINFORMATICS
CONTENTS
STEM CELL STUDIES
MVI-92
13
Lab Diagnostic
Techniques/Instrumentation
MVI-93
14
Biosafety/Bioethics
MVI-94
15
Experimental Therapeutics
MVI-95
16
914
915
916
CODE
920
921
922
923
924
925
CODE
930
931
932
933
934
935
CODE
940
941
942
943
944
945
CODE
950
951
Plant Viruses and Diseases
952
953
954
955
6
CONTENTS
LAB DIAGNOSTICS/INSTRUMENTATION
CONTENTS
ISO-9007
CONTENTS
EXPERIMENTAL THERAPEUTICS
CONTENTS
PLANT VIROLOGY
STRATEGIES TO COMBACT PLANT
VIRUSES
MVI-800: GENERAL VIROLOGY
(3 CREDIT HOURS)
COURSE CONTENTS: This is an introductory level course that describes the history of virology,
development of the concepts of viruses, and various biological and molecular aspects of bacterial,
animal and plant viruses. The course is divided into 3 parts.
COUSRE CONTENTS:

200 years of discoveries

General aspects of virology

Viral Classification and Structure

Modes of infection

Cellular Models of virus propagations

Replication and pathogenesis in a comparative fashion.

Host-virus interactions

Transformation and oncogenesis

Immunopathology

Host defense mechanisms

Antiviral pharmacology and applied virology

Bacteriophages

Plant viruses and their associated diseases

What is next for Virology?
MVI-800: BASIC CONCEPTS IN VIROLOGY (3 CREDIT HOURS)
LEARNIG OBJECTIVES: Viral life cycle, mode of replication and infection will be discussed.
Cytopathic effects of viral infection, lytic and lysogenic infection and viral gene expression and host
defenses will be discussed.
COURSE CONTENTS:

Introduction to Bacteriophages and viruses

Virus Genomes/ Viral Genetics: Linear, Circular, DNA or RNA

Viral Infection: Attachment, penetration, uncoating

Viral Gene Expression: Early and late gene expression

Viral Replication: Lytic and latent life cycles
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
Viral Pathogenesis: Virulence, Cytopathic effects, Host responses

Virus spread through insect vectors

Phenotype mixing and pseuodtypes

Viral Transformation

Prions, Viroids and Virosoids

RNA transforming viruses

DNA transforming viruses

Host Defense Mechanisms

Apoptotic or Anti-apoptotic Viruses

Immune activation

Anti-Viral Pharmacology

Antiviral drugs
BOOKS RECOMMENDED:

Lytic mode of lambda development by D. Freidman and M. Gottesman, cold spring harbor
laboratory, cold spring harbor, N.Y.

Single stranded DNA by R.L. Sinsheimer.

Principles of Virology by S.J. Flint.

Fields Virology by B. Fields

Principles of Virology by Knipe.

Principles of Virology by Alan J. Cann.
MVI-802: ADVANCES IN VIROLOGY
LEARNING OBJECTIVES:
(3 CREDIT HOURS)
Details of the life cycle of different classes of viruses will be
thought. This course is deigned in such as way that students will be able to distinguish target specific
pathways of various classes of viruses. By understanding the common factors that contribute to
diseases caused by viral infections, it will help students to develop strategies to prevent or resolve
such infections.
COURSE CONTENTS
Hepresvirues (HSV-1, HSV-2, HCMV, EBV, PRV, VZV, HSV-6, HSV-8)
Retroviruses (HIV-1, HIV-2, HTLV-1, HTLV-2)
Defective Retroviruses and Endogenous Retrotransposons
8
Hepatitis viruses (HAV, HBV, HCV, HDV, HEV, HGV)
Entroviruses (Coxsackieviruses, Echovirus, Hepatitis A, Norwalk and Norwalk like viruses,
Reovirus, and Rotavirus)
Influenza and cold viruses
Neurotropic viruses [lymphocytic choriomeningitis virus (LCMV) and Measles virus (MV)]
Adenoviruses (Ad-1, Ad-2, Ad-6)
Picornaviruses (Poliovirus, rhinovirus)
Togaviruses (Rubella, Yellow fever, encephalitis)
Polyoma viruses (SV40, JC, and Papilloma)
Coronaviruses
Parvoviruses (ssDNA)
Reoviruses (dsRNA)
Poxviruses
Plus stranded RNA viruses vs. Negative-stranded RNA viruses
Yeast viral like particles (VLPs) (Ty-1, Ty-2, Ty3)
Insect viruses (Dengue, Tick born)
Primate viruses (Ebola and Marburg)
Avian viruses (Bird flu)
BOOKS RECOMMENDED:

Hepatitis B and C carrier to cancer by Sarin.

Retroviruses by Coffin and John M.

Human Retroviruses by Bryan R.

Interaction by retroviruses and Herpes viruses by H. J. Kung.

Human Papilloma viruses by D.J.McCance.

Human Papilloma viruses by Clare (EDT) Davy.

The coronaviridae by Stuart G. Siddenn.

Molecular Biology of Hepatitis B virus by Koshy.
9
MVI-803: MECHANISTIC VIROLOGY
LEARNING OBJECTIVES:
(3 CREDIT HOURS)
Details of the potential mechanism(s) of viral replication and
transcription and translation control will be thought. This course is deigned in such as way that
students will be able to design antiviral approaches that will target specific pathways of viral life
cycle.
COURSE CONTENTS

Oncogenic viruses and potential role of oncogenes

Neurotropic viruses and neurovirulence

Balance between neuroprotection and immunopathogenesis

Mechanism(s) of latency

Packaging/Encapsidation of viral genome

Mechanisms of replication of RNA viruses

Mechanisms of replication of DNA viruses

Control for viral RNA processing

Control of RNA transport and stability

Mechanism of antiviral therapy

Cell to cell spread of viruses

Glycobiology relevant to viral pathogenesis and control

Intracellular Transport mechanism(s) of mature viral particles

Signal transduction pathways perturbed by viral infection and/gene expression

Cellular and animal model of virus propagation

Use of viral vectors as gene therapy

Development of surrogate/pseudo type models for viral genes expression
LAB PRACTICALS
Bacteriophage Isolation (M13, lambda, Mu)
Transduction vs. transposition assay
Purification of replicative forms of M13 ssDNA
Viral isolation and Plaque Assay (Hepresviruses, HSV-1 and HCMV)
10
MVI-804: BACTERIPHAGE BIOLOGY
(3 CREDIT HOURS)
LEARNING OBJECTIVES: Historical overview of pages will be presented. The structural
organization, genetics, attachment, penetration and their replication will be discussed. The
bacteriophages of marine bacteria constitute the highest amount of biodiversity in nature which will
be compared with terrestrial bacteriophage ecology. The use of bacteriophages as therapy to cure
multi-drug resistant bacteria, for cleaning the contaminated polluted rives/garbage will be discussed
COURSE CONTENTS:

General properties of phages

Historical overview of phages

Classification of gram positive and gram negative phages

Large DNA, small DNA and RNA phages

Attachment and Penetration

Synthesis of protein and nucleic acids

Bacteriophage lysis: Mechanism and regulation

Phages and transposable elements (Mu Biology)

Lambda phage

T3, T7 and T4 phages

P1 (Cre lox) system

Defective phages and phage-like objects

Evolution and natural biology of phages

Evolutionary conservation and relation to eukaryotic viruses

Relation of bacteriophages to bacterial ecology

The health value of bacteriophages (Phage Therapy)

Synthetic bacteriophages

Use of bacteriophages in Physics (Nano wires and electrodes)

Biocide bacteriophages for environmental surfaces

Bacteriophages of industrial importance

Control of bacteriophage contamination
11
MVI-810: MOLECULAR BIOLOGY
(3 CREDIT HOURS)
LEARNING OBJECTIVES: This course will discuss basic principles of molecular biology,
structures of macromolecules with emphasis on the mechanisms that dictate the fundamental
processes for the transfer of DNA->RNA-> protein.
COURSE CONTENTS:

Structure and origin of RNA and DNA

Molecular structure and organization of Genes, Genome and Chromosomes

Gene Transcription

Regulation of Transcription Initiation, role of GTF and TSFs

DNA Replication

DNA Recombination (site specific and non-homologous)

RNA Processing, Nuclear Transport, and Post-Transcriptional Control

Control of Translation

Selection of mRNAs to be translated

mRNA degradation and interference

Protein activity: Post-translational modifications, activation, localization, and degradation

Protein structure and function
LAB PRACTICALS
Nucleic Acid Biochemistry and Analysis:

DNA isolation (Plasmid and Chromosomal)

Agarose and Polyacrylamide Gel Electrophoresis

Electrophoresis Mobility Shift Assays (EMSA)

Chromatography

Spectrophotometer Techniques
Molecular Cloning and subcloning

Vector preparation, restriction mapping

Polymerase chain reaction (PCR) of a target gene

Agarose gel electrophoresis of gene PCR product
12

Analysis of restriction digest, ligation into a vector

Transformation to E. coli and yeast

Selection and Screening, Plasmid preparation and large scale purification

Southern Blot vs. Northern blot analysis

DNA sequencing and Alignments-Review bioinformatics tools

Protein Translation: Open Reading Frames
BOOKS RECOMMENDED
Molecular Biology of the Gene by Watson, J. D., T. A. Baker, S. P. Bell, A. Gann, M. Levine, and
R. Losick, Eds., (2003). (5th edition) New York, Benjamin Cummings ISBN 0-8053-4635-X
The Cell by Bruce Albert and Dennis Bray, (3rd Edition), Garland Publishing Inc, New York and
London Gene VIII By Lewin Benjamin Eds 2004. Oxford University press, Inc, New york.
Biochemistry by Victor L. Davidson, Donald B. Sittman. 3rd Edition, 1993, Harwal Publication Co.
Cell and Molecular Biology by Gerald Karp.1996 John Willey and Sons, Inc. London.
Genetics by Peter J. Russel. 5th Ed. 1997, Benjamin-Cummings Publishing Company.
MVI-811: CELL BIOLOGY-I
(3 CREDIT HOURS)
LEARNING OBJECTIVES:
This course is designed to help students understand workings of the cell structure and function at
cellular and molecular level. In this course molecular basis of cell structure and function is
discussed which will provide students a detailed account of functional physiology of the cells.
COURSE CONTENTS:

Introduction

Cell Organization

Cell Architecture

Membrane Structure and Function

Bio Transport

Vesicular Transport

Transport Signals

Nuclear Transport

Bio Energetic

Mitochondrial Energy Conversion
13

Chloroplast Energy Conversion

Cytoskeleton

Cell Shape

Cell Contractility

Cell to cell Communication

Electrochemical Signaling

Synaptic and Sensory Transduction

Biochemical Signaling

Receptor Ligand Interactions

Second Messengers

Signaling Cascades

Cell Cycle and Apoptosis

Phases of Cell Cycle and Cell Division

Regulation of Cell Growth and Death
 Specialized Cell Systems
BOOKS RECOMMENDED:
Gene VIII By Lewin Benjamin, Eds 2004. Oxford University press, Inc, New york.
Molecular Biology of the Gene by Watson, J. D., T. A. Baker, S. P. Bell, A. Gann, M. Levine, and
R. Losick, 5th Ed. 2003. New York, Benjamin Cummings ISBN 0-8053-4635-X
Molecular Biology of the Cell by Bruce Albert and Dennis Bray, 3rd Ed. Garland Publishing Inc,
New York and London.
Biochemistry by Victor L. Davidson, Donald B. Sittman. 3rd Ed. 1993, Harwal Pub Co.
Cell and Molecular Biology by Gerald Karp.1996 John Willey and Sons,Inc. London.
MVI-812: MEMBRANE TOPOLOGY & SIGNAL TRANSDUCTION
(3CREDIT HOURS)
LEARNING OBJECTIVES:
This course is designed to help students understand workings of the cell structure and function at
cellular and molecular level that various viruses utilize in their life cycles. In this course molecular
basis of cell structure and function is discussed which will provide students a detailed account of
14
functional signaling cascade physiology of the cells. Furthermore, this course will discuss the key
components of the immune system at cellular and molecular level.
COURSE CONTENTS:

Cell Adhesion and ECM

Cell adhesion molecules and protein targeting

Collagen Matrix and Non-collagen Matrix

Metastasis

Protein sorting

An overview of protein targeting

Peroxisomal protein targeting

Secretary protein targeting

Membrane proteins

Functions of cell surface receptors

Post-translation modifications

Golgi protein sorting

Vesicular Transport

Cytoskeleton and force generation

Actin dynamics and Actin myosin interaction and regulation

Microtubule dynamics (dynamic instability model)

MAPS and microtubule motors

Cilia and flagella structure and movement

Pathways of intracellular signal transduction

P38 MAPK, JNK, ERK pathways

RAS and RAF pathways

Oxidative stress and anti-stress signaling (GST, MnSOD)

GTPase and their role in cancer

Ion channels expression, regulation and its relations to diseases

Purinergic signaling (p2X and p2Y)

PKA, PKB (AKT) PKC pathways
15

JAK/STAT and TGF- /smad pathways

NF- B signaling

Hedgehog Wnt and Notch pathways

G-Protein coupled membrane receptors signaling

Steroid hormones and nuclear receptors signaling

Neurotransmitters, Opioids receptors and Eicosanoids signaling

Peptide hormones and growth factors signaling

Feedback and crosstalk signaling network

Cell Signaling and its Applications in Disease and Therapeutics
CELL BIOLOGY LAB PRACTICALS

Cell and Tissue Culture

Membrane fractionation

Transient and Stable DNA Transfections

Tissue processing

Immunohistochemistry

Microscopy

Chromatin Immunoprecipitation
(01 CREDIT HOUR)
BOOKS RECOMMENDED:
Gene VIII By Lewin Benjamin Eds 2004. Oxford University press, Inc, New york.
Molecular Biology of the Gene by Watson, J. D., T. A. Baker, S. P. Bell, A. Gann, M. Levine, and
R. Losick, 5th Ed. 2003. New York, Benjamin Cummings ISBN 0-8053-4635-X
The Cell by Bruce Albert and Dennis Bray, 3rd Ed. Garland Publishing Inc, New York and London.
Biochemistry by Victor L. Davidson, Donald B. Sittman. 3rd Ed. 1993, Harwal Pub Co.
Cell and Molecular Biology by Gerald Karp. 1996, John Willey and Sons,Inc. London.
NCVI-813: GENOMICS AND PROTEOMICS
16
(3 CREDIT HOURS)
LEARNING OBJECTIVES: This course aims at offering an insight into genomics and proteomics
of biological systems, their diversity and various aspects as well.
COURSE CONTENTS:

Yeast genome as a model eukaryotic genome system

Genome diversity and evolutionary time-scale

Human Genome project, it implications on viral infections

Quantitative genetics

Linkage, crossing over and gene mapping in Prokaryotes

Linkage, crossing over gene mapping in Eukaryotes

Introduction to DNA microarrays

Expression profiling using microarrays

DNA microarray experimental design

DNA microarray data analysis and interpretation

Application of functional genomic research in the study of viruses

Proteome analysis

Techniques of proteomics

Proteomes in health and viral disease

Limitations and future development
BOOKS RECOMMENDED
Genomics, Proteomics and Bioinformatics by A. Malcolm Campbell and Laurie J. Heyer. 2002.
Benjamin Cumming Publishing Company.
Proteomics: From protein sequence to function by Pannington, S and M.J.Dunn. 2001. Spring
Verlag.
Introduction to Proteomics, Tools for the New Biology by Daniel C. Liebler. 2001. Humana
Press.
DNA Microarray: A molecular cloning manual by David Bowtell and Joseph Sambrook. 2003.
Cold Spring Harbor Laboratory Press, New York.
17
MVI-820: MOLECULAR MICROBIOLOGY

(3 CREDIT HOUR)
LEARNING OBJECTIVES: This is an introductory level course that describes various
biological and molecular aspects of bacteria. Our coverage will focus almost entirely on
bacteria that infect humans and cause serious disease.

COURSE CONTENTS:

The Bacterial Cell: An introduction to the structure of the bacterial cell.

Bacterial identification in the diagnostic laboratory versus taxonomy.

Taxonomic characterization of bacteria. Approaches to rapid diagnosis

Nutrition, Growth and Energy Metabolism: Anaerobic and aerobic metabolism. Metabolism
of sugars and fatty acids

Cell Envelope, spores and Macromolecular Biosynthesis: Structure and synthesis of the cell
walls of gram-positive and gram negative bacteria

Antibiotics - Cell Envelope: The mode of action of beta-lactate antibiotics.

Antibiotics - Protein Synthesis, Nucleic Acid Synthesis and Metabolism: The mode of action
of antibacterial chemotherapeutic agents. Antibiotic susceptibility testing.

Genetic Exchange: The mechanisms of gene transfer in bacteria; Insertion sequences,
transposable genetic elements and plasmids.

Genetic Regulatory Mechanisms: The structure and transcription of bacterial genes. The
molecular mechanisms that bacteria use to regulate gene activity. Inducible and repressible
operons. The molecular mechanisms involved in catabolite repression and attenuation. The
ways bacteria regulate enzyme activity.

Enterobacteriaceae: Enterobacteriaceae, Vibrio, Campylobacter and Helicobacter

Streptococci: Groups A, B and D streptococcus, pathogenesis, diagnosis. Streptococcus
pneumonia and Staphylococci. Streptococcus and pneumonia, Staphylococcus infections,
food poisoning, toxic shock Neisseria and Spirochetes: Syphilis, Lyme disease,
leptospirosis, gonorrhea, meningitis.

Anaerobes and Pseudomonas - Opportunistic Infections. Clostridia, gas-gangrene, tetanus,
botulism, pseudomonads.

Zoonoses: Listeria, Francisella, Brucella, Bacillus and Yersinia Plague, Anthrax,
Brucellosis, Listeriosis.
18

General Aspects of Bacterial Pathogenesis: Exotoxins and endotoxins
LAB PRACTICALS

General Microbiological Laboratory Techniques

Media preparation

Identification of gram negative and positive strains

Growth curves for bacteria

Bacteriophage isolation

Bacterial conjugation for Plasmids transfer

Genetic complementation

Biosafety Guidelines
BOOKS RECOMMENDED
Medical Microbiology & Immunology by Warren Levinson & Ernest Jawetz. 7th Ed. 2003.
McGraw-Hill Publications. ISBN 0-07-122973-6.
Principles of Virology, Molecular Biology, Pathogenesis, and Control by S.J. Flint, L.W.
Enquist, R.M. Krug, V.R. Racaniello, and A.M. Skalka. 2nd Ed. 2000. ASM Press.
Fundamental Virology edited by D.M. Knipe and P.M Howley. 4th Ed. 2001, Lippincott Williams
and Wilkins.
MVI-830: BASIC IMMUNOLOGY
LEARNING OBJECTIVES:
(3 CREDIT HOURS)
This course will cover basic concepts of immunology and the
mechanism by which T and B lymphocytes acquire high level of specificity. Surface molecules will
be described in detail, followed by accessory and natural killer cells and human lymphocyte antigen.
Autoimmunity and tolerance will also be discussed.
COURSE CONTENTS:

The immune system

Origins of the immune system

Antibody structure and function and antibody diversity

Helper T cells and their activation

Organs of the lymphatic system
19

T lymphocyte development and differentiation

B cell lymphocyte Development and Differentiation

Cell-cell interaction (T cells and Antigen presentation cells)

Non specific Defense Cells (Natural Killer cells)

Monocytes and Dendritic cells

HLA system (genomic organization, molecule structure, class I/II alleles and antigen
presentation).

The complement system

Innate immunity

Leukocyte migration

Pathological Immune mechanisms and tolerance
BOOKS RECOMMENDED
Introduction to Immunolgy by J.W. Kinhall, 1983, Macmillan Pub Co.
Immunology by Richard A.Goldsby, Thomas j.Kindt, Barbara A. Osborne, Janis Kuby, 2005. W.H
Freeman and Co.
Cellular and Molecular Immunology by Abbas, 2005. Elsevier Pub Co.
Color Atlas of Immunology by G.R. Burmester, A. Pezzutto, 2006. Thieme Stuttgart, New York.
MVI-831: LABORATORY APPLICATIONS OF IMMUNOLOGY
( 3 CREDIT HOURS)
COURSE OBJECTIVE: This course is designed to describe techniques for antibody production,
antigen detection, Immunofluorescence, and Immunohistochemistry.
COURSE CONTENTS

Antibody production (monoclonal and polyclonal)

Antigen-Antibody interaction

Definitions and precipitation techniques

Techniques of electrophoresis

Agglutination Techniques/complement binding Reaction

ELISA, RIA, and Immunoblotting

Immunrofluorescence, Immunohistology

Cellular Immunity
20

Cell Isolation techniques

Test of T cell Functions

Antigen-specific Test

Assay procedure for characterizing antigen specific T cells

Humoral Immunity

Test for B-cell Function

Molecular Biological Methods

Analytical Techniques
MVI-832: IMMUNE DISORERS.
(3 CREDIT HOURS)
LEARNING OBJECTIVES: Major human diseases attributed due to defects in immune system
will be discussed and immunological mechanism that governs the onset of key human diseases will
be eluded for their potential as therapeutic potential. Genes that contributes for these defects will
also be studied.
COURSE CONTENTS

Immunodeficiencies (Sever combined immunodeficiency syndrome)

Humoral and cellular Immunodeficiencies

Granulocytic Deficiencies

Complement Deficiencies and Defects

HIV Structure and Replication

Course of HIV Infection

Diagnosis and Treatment of HIV Infection

Hemolytic diseases and cytopenias

ABO blood group system Rhesus and other blood group systems

Mechanisms of hemolytic and antigen detection autoimmune diseases

Drug induced hemolysis

Hemolytic due to warm antibodies

Hematological Diseases

Acute Leukemias

Overview of Lymphoma Classification
21

Hodgkin’s disease

T-Cell Lymphomas

B-Cell Lymphomas

Plasma Cell Dyscrasias

Multiple Myeloma

Cryoglulinemia

Amyloidsis

Tumor Immunology

Detection and identification of Tumor Antigens

Immune Escape Mechanisms of Tumor Antigens

Immunotherapeutic Strategies (I)

Immunotherapeutic Strategies (II)

Transplantation of Autologous

Bone Marrow/Heatopoietic Stem Cells

Transplantation of Allogenic bone Marrow/Hematopietic Stem Cells

Clinical Aspects of Organ Transplantation
MVI-833: CLINICAL IMMUNOLOGY 2
(3 CREDIT HOURS)
COURSE OBJECTIVE: Major human diseases attributed due to defects in immune system will be
discussed and immunological mechanism(s) that governs the onset of key human diseases will be
elucidated for their therapeutic potential.
COURSE CONTENTS

Musculoskeletal Diseases

Clinical features of Rheumatoid Arthritis

Synovial Changes in Rheumatoid Arthritis

Pathogenesis of Rheumatoid Arthritis

Juvenile Chronic Arthritis

Clinical features of Spondylarthritis

Pathogenesis of Spondylarthritis

Gout, Ploychondritis, and Bechet’s syndrome
22

Skin Diseases

Urticaria

Contact Allergies

Atopic Dermatitis and Leukocytoclastic vasculities

Psoriasis and Bullous Skin Diseases

Gastrointestinal Diseases

Atrophic Gastritis, Whipple’s disease and Sprue

Chronic Inflammatory Bowl Diseases

Autoimmune Liver Diseases

Respiratory Diseases

Bronchial Asthma and Allergic Rhinitis Sarcoidosis and Idiopathic

Pulmonary Fibrosis

Extrinsic Allergic alveolitis Tuberculosis

Renal Diseases

Immunological mechanisms

Glomerulonephritis (I)

Glomerulonephritis (II) and Interstitial Nephritis

Metabolic Diseases

Autoimmune Thyroid Diseases

Diabetic Mellitus and Autoimmune Polyglandular Syndrome

Heart disease

Rheumatic fever, Myocarditis, and Postinfection syndrome

Neurolgical Diseases

Multiple Sclerosis

Autoantibody-mediated Diseases

Myasthenia Gravis and Lambert-Eaton syndrome

Ophthalmic Diseases

Anatomy and Pathogenesis

Extraocular inflammations

Uveitis (I)
23

Uveitis (II) and Ocular Manifestation of Systemic Disease

Reproduction Immunology

Reproduction immunology

Gene therapy

Modern approaches and tools for gene therapy
JOURNALS RECOMMEDED
ANNUAL REVIEW OF IMMUNOLOGY, JOURNAL OF IMMUNOLOGY, EUROPEAN
JOURNAL OF IMMUNOLOGY, IMMUNOLOGY TODAY, ADVANCES IN IMMUNOLOGY,
CRC CRITICAL REVIEWS IN IMMUNOLOGY
and HUMAN GENE THERAPY
MVI-840: PHARMOCOLOGY (Viral, Immune)

Immune Pharmacology

(NSAIDS) and Glucocorticoids

Antimetabolites, Cyclophosphamide, Sulfasalazine, and Gold

Cyclosporin A, Mycophenolate, and Leflunomide

Nucleoside and non-nucleoside analogs

ATP-signaling and Viral response

Antiretroviral update

Xenobiotic Transport defects

Side effect of drugs and liver metabolism

Troxacitabine in acute leukemia

Varicella vaccine and fatal outcome in leukaemia
MVI-850: GENERAL GENETICS
(3 CRDIT HOURS)
(3 CREDIT HOURS)
LEARNING OBJECTIVES: Medical genetics involves any application of genetics to medical
practice. The objectives of this course is to provide students with breadth and depth of knowledge to
make them realize the role of genetics in medicine and why the knowledge of medical genetics is
important for today’s health care facilitators and also for scientists and clinical researchers who are
interested in conducting research to elucidate the genetic basis of inherited disorders whose numbers
is increasing rapidly.
24
COURSE DESCRIPTION:
This course includes detailed presentation of structure and function of genes, DNA analysis,
chromosomes and chromosomal aberrations; gametogenesis, Mendalian versus Non-Mendelian
inheritance; genomics and population genetics.
COURSE CONTENTS:
Medical Genetics in Perspective:

Developmental genetics-basic concepts

The human chromosomes

The life cycle of somatic cell

Meiosis

Human gametogenesis and fertilization

Medical relevance of mitosis and meiosis

The Human Genome: Structure and Function of Genes and Chromosomes:

DNA structure; A brief view

The central dogma: DNA RNA

Fundamentals of gene expression

Structure of human chromosome

Variation in gene expression and its relevance in medicine

Techniques of Gene Analysis:

Analysis of individual DNA and RNA sequences

Methods of nucleic acid analysis

In Situ Hybridization to chromosomes

DNA sequence analysis

Methods of protein analysis

Pattern of Single-Gene Inheritance:

Terminology

Genetic disorders with Classical Mendelian Inheritance

Autosomal recessive inheritance

Pattern of autosomal dominant inheritance

X-linked inheritance
Protein
25

Atypical pattern of inheritance

Genetic Variation in Populations:

Genetic diversity in human populations

Phenotypes, genotypes and gene frequencies

The Hardy-Weinberg law

Frequency of X-linked genes and genotypes

Factors that disturb Hardy-Weinberg equilibrium

DNA profiling- a practice test of Hardy-Weinberg assumptions

Gene Mapping and Human Genome Project:

Physical mapping of human genes

Mapping human genes by linkage analysis

Applications of human genome mapping

The human genome project
RECOMMENDED BOOKS:
Essential of Genetics by William Klug, Michael Cummings. Pearson Pretice Hall 2005.
Human Genetics by Ricki Lewis. McGraw Hill 2005
Genetics in Medicine: Robert Nussbauon, Roderide Mclnnes by Huntington Willard Thompson and
Thompson. Saunder 2004
Medical Genetics by Lynn Jorde, John, Michael Bamshad, Raymond White. Mobsy 2003.
Medical Genetics at a Glance by Dorian Pritchard, Bruce Korf. Blackwell S. Publications 2003.
MVI-851: MEDICAL GENETICS
(3 CREDIT HOURS)
LEARNING OBJECTIVES: Emphasis is placed on the clinical application of Genetics. Such
topics include genetic assessment and counseling with prenatal diagnosis; chromosomal disorders;
single gene defects attributed due to viral integration; immunogenesis and cancer genetics;
multifactorial disorders congenital malfunctions; population screening; prevention and control;
treatment of genetic autoimmune immune diseases.
Clinical Cytogenetic:

Introduction to Cytogenetic

Chromosomal abnormalities
26

Mendelian disorders with Cytogenetic effects

Cytogenetic analysis in cancer

Disorders of autosomes and sex chromosomes
Principles of Molecular Diseases:

Effect of mutation on protein function

Mutations disrupting the functions of biologically normal proteins

Hemoglobinopathies and HIV/HCV infection correlations
Molecular and Biochemical Basis of Genetics:

Diseases due to mutations in different classes of proteins

Enzyme Defects critical for viral infection

Defects in viral receptors proteins (chemokine receptors)

Disorders of structural proteins

Neurodegenerative disorders

Pharmacogenetic diseases
Genetics of Disorders with Complex Inheritance:

Genetic analysis of qualitative disease traits

Genetic analysis of quantitative traits

Genetic mapping of complex traits

Diseases with complex inheritance
Cancer Genetics:

Cancer as a genetic disorder

Characteristics of cancer cells

Genes that cause cancer-oncogenes; tumor suppressor p53, WLM, Rb, BRCC1

Genetic changes that cause some cancer-case studies

Cancer prevention, diagnosis and treatment
Diagnosis Strategies for Genetic Disorders:

Indication for prenatal diagnosis

Genetic counseling for prenatal diagnosis

Methods of prenatal diagnosis

Emerging technologies for prenatal diagnosis
27

Laboratory investigations

Effect of prenatal diagnosis on the prevention and management of genetic diseases

Population screening (neonatal screening)

Overall impact of genetic disease

Types of genetic diseases

Current state of treatment of genetic diseases

Special consideration in treating genetic diseases

Treatment strategies

Genetic Counseling and Risk Assessment:

Genetic counseling

Case management in genetic counseling

Determining recurrence risks

Genetics and Society:

Population screening for genetic diseases

Ethical issues in medical genetics

Eugenic and dysgenic effects on gene techniques
RECOMMENDED BOOKS:
Genetics in Medicine by Robert Nussbauon, Roderide Mclnnes. Huntington Willard Thompson and
Thompson. Saunder 2004.
Essential of Genetics by William Klug, Michael Cummings. Pearson Pretice Hall 2005.
Human Genetics by Ricki Lewis. McGraw Hill 2005.
Medical Genetics by Lynn Jorde, John, Michael Bamshad, Raymond White. Mobsy 2003.
Medical Genetics at a Glance by Dorian Pritchard, Bruce Korf. Blackwell S.
MVI-852 IMMUNOGENETICS
(3 CREDIT HOURS)
INTRODUCTION

Genetics of AB and TCR genes

Mechanism of AB diversity

Somatic recombination

Somatic hypermutation
28

B-cell receptor genes

T-cell receptor genes

RAG genes, Knockout mice

Inherited immune disorders.

Hyper Sensitivity

Autoimmunity

Immune Suppression.
MVI-860: VIRAL PATHOLOGY
LEARNING OBJECTIVES:
(3 CREDIT HOURS)
Major objective of this course will be to understand the viral
induced pathogenesis. The symptomatic of the disease and its progression into fatalities will be
discussed. The molecular pathways that lead to the induction of viral induced oral hairy leukoplakia,
insulin resistance, encephalitis, demyelination hemophagocytic Lymphohistiocytosis, choleostasis
hemorrhagic fever, hepatic fibrosis, cirrhosis and hepatocellular Carcinoma (HCC) will be studied.
COURSE CONTENTS

HCV -induced hepatic fibrosis

Viral induced fatty liver diseases (steatosis)

Hepatocellular carcinoma (HCC)

Viral induced arthrosclerosis

Viral induced ascitis

Viral induced insulin resistance

Viral meningitis and encephalitis

CMV retinitis

Falvi and foliovirus induced hemorrhagic fever

Antibody dependent enhancement (ADE) of Dengue Virus

Oncogenic viral pathology

Viral induced choleostasis

HIV/EBV induced Oral Hairy Leukoplakia (OHL)

Kaposi’s Sarcoma

Viral induced karatitis and conjunctivitis
29

Viral induced myocarditis and inflammatory cardiomyopathy

Viral induced Emphysema

Virus induced autoimmune diseases

Virus Induced Demyelination

EBV-induced Hemophagocytic Lymphohistiocytosis
MVI-870: VACCINOLIOGY
(3 CREDIT HOURS)
LEARNING OBJECTIVES: This course is deigned in such as way that students will be able to
design vaccines and their testing in yeast and mammalian cell, eggs and various animal models. The
history of vaccine, their efficacy, potential draw back/risks, impurities, cost effective, methods will
be discussed.
COURSE CONTENTS

History of vaccines

Viral proteins as potential targets for vaccine

Live versus attenuated vaccines

Role of multinational companies in vaccines production

DNA as vaccine

Peptide & Subunits vaccine

Adjuvants in vaccines

Population Genetic analysis: immunity to vaccine

Recombinant vaccine

Animal models of vaccine testing

Cost-effective approaches for production of new vaccines

Human testing and efficacy of vaccines: ethical issues

Recommendations of the Advisory Committee on Immunization Practices (ACIP)

Quadrivalent vaccine against human papillomavirus to prevent high-grade cervical lesions

Understanding the demand and supply of popular vaccine

Launching of vaccine and clinical trials

Anti-rabies immunoglobulin preparation based on F(ab')2 fragments

Effect of Panavir/Zanamivir on influenza A virus reproduction
30

Vaccine safety
RECOMMENDED BOOKS:

Recombinant vectors in vaccine development by Fred Brown.

The Vaccine book by Barry R. Bloom.

Vaccine S by Plotkin_Orenstein.

Mass vaccine global aspect-progress and obstacles by Plotkin.

Pre-clinical and Clinical development of new vaccines by Fred Brown.
JOURNALS RECOMMENDED: VACCINE, JOURNAL OF ANTIMICROBIAL
CHEMOTHERAPY, CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY, MEDICAL
MICROBIOLOGY AND IMMUNOLOGY
MVI-880: MOLECULAR TOXICOLOGY
(3 CREDIT HOURS)
LEARNING OBJECTIVES: This course will provide a general understanding of the science of
toxicology as it relates to mankind and the environment at macro and molecular levels.
COUSE CONTENTS:

Fundamental concepts of Toxicology

Dose-response relationships

Effect of and viral infection and vaccines on liver toxicity

Absorption of toxicants, distribution and storage of toxicants

Biotransformation and elimination of toxicants.

Classes of toxins and their respective toxic effects on humans, animals and the environment.

The mechanisms of toxicity, describes medical, biochemical and genetic aspects of toxicology
including regulation of gene expression

Regulatory Toxicology, i.e., risk assessment and the chemo dynamics of viral contaminants in
the environment.
COURSE CONTENTS:
Part I: Basic Concepts

History and Basic Principles of Toxicology

Measurement of Toxicity: Toxicokinetics during viral infection

Absorption, Distribution, Storage, Elimination

Biotransformation: Metabolism and bioactivation of toxins
31

Part II. Various classes of Toxicants:

Chemical Toxins

Toxicology of gaseous pollutants

Hydrocarbons

Halogenated hydrocarbons

Aromatic amines and N-nitroso compounds

Metals

Polymers

Cosmatics

Biochemical toxins

Insecticides and pesticides

Animal venoms and poisons

Plant toxins

Microbial toxins

Food and water borne toxins

Drugs and biopharmaceuticals
Part III. Mechanisms of Toxicity:

Organ System Toxicology

Hematotoxicity

Hepatotoxicity

Pulmonotoxicology

Nephrotoxicology

Neurotoxicology

Dermatotoxicology

Oto-oculartoxicology

Immunotoxicology

Genotoxicology

Carcinogenesis: DNA damage, DNA repair

Toxicogenomics: Induction and regulation of gene expression by toxins
Regulatory Toxicology:
32

Toxicity assays

Risk Assessment

Controlling health hazards

Detoxification during and after viral infections

Bioremediation

Phytoremediation
BOOKS RECOMMENDED
Principles of toxicology by Williams and Roberts. 2nd Ed. A wiley Interscience publication.John
wiley and sons, Inc, New york
Toxicology by Hans Marquardt, Siegfried Schafer, Roger O. McClellan 1999. Academic Press Inc.
London.
Industrial Toxicology by Williams and Burson. 1989. John Willey and Sons.Inc.
Environmental Toxicology by Sethi, Iqbal, Satake, Mido. 2nd Ed.
Casarette & Doull’s Essentials of Toxicology by Curtis D.Klassen. John B. Watkins. 2003. Mc
Graw Hill.
Molecular Toxicology by P. David Josephy. Oxford University press.
Handbook of Toxicology by Michael Derelanko and Manfred Hollinger CRC Press 2004.
MVI-890: BIOTECHNOLOGY
(3 CREDIT HOURS)
LEARNING OBJECTIVES: This course addresses the isolation, genetic manipulation, use of
organisms (commonly genetically modified) or their fermented food production, agriculture,
pharmaceutical discovery and production, molecular diagnosis, vaccine production, transgenic
animal formation, human gene therapy, forensic applications, microbial-base bioprocessing
pharming, bioterrorism and future of biotechnology. The course will consist of three parts:
COURSE CONTENTS:

Approaches of biotechnology

Specific applications

Social issues
I. APPROACHES OF BIOTECHNOLOGY:
33
Structural Genomics:

Genome sequencing

Protein structure determination
Functional Genomics:

Bioinformatics

DNA microarray

Proteomics
ii)
Modifying Protein Production and Function:

Protein engineering

Antisense technology
II.
SPECIFIC APPLICATIONS:
Food Biotechnology:

Viral infection in food

Animals and microbes
Human Health and Diagnosis:

Viral diagnostics.

Forensic applications
Industrial Applications:

Bioprocessing

Microbial-based bioprocessing and Farming

Biosensors for detection of viruses

Recovering viral genomes and sequences
Environmental Applications of virus infection:

Bioremediation
34

Waste management with virus contamination

Phytoremediation

Biofertilizers
SOCIAL ISSUES:
Rights and Privileges:

Intellectual property

Ethical implications

Risk and regulations
Perception and Fears about Viruses and Biotechnology:

Contrasting public and scientific perceptions of vaccines

Bioterrorism and bio defense
Biotechnology: The Future:

Purpose and expected outcomes

Technological advances in the pipeline

Third world participation in vaccine and biotechnology-bridging the gap
RECOMMENDED BOOKS:
Understanding Biotechnology: George Acquaah, Pearson Prentice Hall (2004).
MVI-900: BIOSTATISTICS
LEARNING OBJECTIVES:
(3 CREDIT HOURS)
This course is designed for students to understand different
computational biological algorithms, construct data models, use of molecular databases, use of
commonly available software for the analysis of bimolecular sequences and structures in viral and
cellular genes. They should be able to interpret the results, describe common post-genomic
experimental technologies associated with high-throughput data production.
COURSE CONTENTS:

Descriptive Biostatistics

Types of numerical data

Data sampling methods
35

Graphical display of Data

Measurement of central tendency

Measurement of dispersion

Probability and probability distributions

Chi-Square Test

T-Test

Module II: Inferential Biostatistics

Sampling Distributions

Confidence Intervals

Baye’s Theorem

Hypothesis Testing

Experimental Design

Introduction to operating systems

Basics of Linux operating system

Words Processing, Spreadsheet and Presentation Skills

Internet for Biologists

Introduction to programming languages

Introduction to perl programming language

Programming skills in perl

Perl applications in bioinformatics

Introduction to databases

COBRA – Common Object Request Broker Architecture

MySQL database management system
MVI-901: BIOINFORMATICS
Module I:
( 3 CREDIT HOURS)
Introduction to Bioinformatics

Definitions of bioinformatics and related fields

Objective and scope of bioinformatics

Kind of data used in bioinformatics

Data integration and data analysis
36

Major biological databases
Module II:
Sequence Alignment

Pairwise Sequence Alignment

Methods and tools of sequence alignment

Multiple Sequence Alignment
Module III: Phylogenetic Analysis

Phylogenetic trees

Methods of Phylogenetic analysis

Methods of evaluating phylogenies
Module IV:
Gene Prediction

Introduction

Methods of gene prediction

Gene prediction tools
Module V:
Protein Folding/Structure

Protein structure and its classification

Protein classification methodology and tools

Protein structure databases and visualization tools

Protein structure prediction

Protein function prediction
Module VI: Genomics and Proteomics

Introduction to genomics

Tools for genome analysis

Approaches for Genome-wide scan

Introduction to proteomics

Tools for proteome analysis
37
LAB PRACTICLES:

Familiarization with Linux OS

Introduction to perl scripting

Creating a simple bioinformatics database

Accessing the publicly available databases

Pair wise and multiple sequence alignment

BLAST database and genome annotation

An Introduction to the vector/primer design Program

Protein structure prediction for known folds

Protein structure prediction for unknown folds

Visualization of biomolecular structures
BOOKS RECOMMENDED:
Fundamentals of Biostatistics by Bernard Rosner, Duxbury 2000.
An Introduction to Biostatistics by Glover & Mitchell, McGrawHill 2002.
Beginning of Perl for Bioinformatics by J Tisdall, O’Reilly 2002.
Bioinformatics: Sequence and Genome Analysis by DW. Mount, Clod Spring Harbor 2002.
Bioinformatics: Genes, Proteins & Computers by C.A. Orengo, Advanced Text 2003.
Bioinformatics: A practical guide to the analysis of genes and proteins by Baxevanis &
Ouellette, Wiley 2005.
TUTORIAL/ PRESENTATION
(2 CREDIT HOURS)
SKILL ENHANCEMENT
LEARNING OBJECTIVES: This is a presentation skill enhancement course, which will enable
students to prepare seminar of assigned topics covering recent knowledge in the field. Evaluation
will be based on the quality of the review, oral presentation and comprehension of the subject. The
exact details of the activities will be described by the faculty accordingly.
38
MVI-903: RESEARCH SEMINAR.
(2 CREDIT HOURS)
PROPOSAL ASSIGNMENTS
LEARNING OBJECTIVES: NCVI will encourage working with clinicians and/other scientists to
develop translational overlaps so the field of investigation could be enhanced. This is a skill
enhancement course, which will enable students to participate in to variety of activities of their
choice. These will include either preparing a review in a given subject or preparing a research
proposal covering recent knowledge in that field. The exact details of the activities will be described
by the faculty time to time accordingly.
MVI-910: STEM CELL STUDIES
(3-CREDIT HOURS)
This course is designed to educate students about the new concepts of stem cell research. Primary
cell culture techniques, pluripotent cell isolation, dentriatic cells injection techniques will be taught.
COURSE CONTENTS

Hematopiotic stem cell/lineage

Bone marrow transplantation

Embryonic vs. Endodermal lineage.

Mesenchymal vs. Neural lineage

Proliferation markers

Cytokines & growth factor for stem cell culture

In vitro cell tracking (flowcytometry/FACS)

Chemical labeling of proteins and cells

Stable isotope labeling in culture medium

Bead based separation systems

Fluorescent and Colorimetric labeling & detection

HLA-typing

T-cell receptor gene transfer by lentiviral vectors in adoptive cell therapy

Karyotyping.
MVI-920 LAB DIAGNOSTICS/INSTRUMENTATION
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(3 Credit hours; TO BE OFFERED)
MVI-930 BIOETHICS/BIOSAFETY
(3 Credit hours)
LEARNING OBJECTIVES: This course will be offered into 2 parts. First part is designed to
educate students about work ethics, creating a culture for dedicated research, honesty/scientific
credibility, sexual harassment, clinical misconduct, patients confidently and plagiarism. Second part
will deal with safe handling of key chemicals, solvents, safe acids, base, liquid nitrogen fire, viruses,
bacteria and carcinogens. Proper Instrumental handling to protect oneself in case of accidents
MVI-940: EXPERIMENTAL THERAPEUTICS
(3 Credit hours)
LEARNING OBJECTIVES: This course is designed to search for the potential approaches that
will arrest the viral-induced pathogenesis and leukemia. The oxidative and anti oxidative signaling
pathways that are perturbed in many viral and immune diseases will be studied.
COURSE CONTENTS:
 Introduction

Experimental drug design

A virological benefit from an induction/maintenance strategy: the Forte trial

Interferon: cellular executioner

Therapeutic issues in HIV/HCV-coinfected patients

Tumor therapy mediated by lentiviral expression of shBcl-2 and S-TRAIL

Antihyperalgesic effect of a recombinant herpes virus

Silymarin (milk tussle) as potential therapeutics for oxidative stress

Flavinoid and Opinopid compounds as antiviral

Antioxidant therapies for cancer and viral infection (SAM, NAC)

Mucosal immunotherapy for alzheimer's disease with viral vectors

Genetic polymorphisms in the chemokine and chemokine receptors

Transition metal therapies during viral infection (zinc supplementation)

Role of cyclic nucleotide phosphodiesterase inhibitors as novel therapeutic agents for the
treatment of human lymphoid malignancies.

PDE4 inhibitors in primary human chronic lymphocytic leukemia (B-CLL)

AND-34, an SH2 domain-containing protein their role in viral diseases
40

Focal adhesion protein p130Cas and role in human breast cancer cells

Hsp90, thioredoxin reductase, and other cytosolic translocation factors as therapeutics

Caspase and protease inhibitors to arrest viral replication

SiRNA/ShRNA and antisense approaches to arrest gene expression
BOOKS RECOMMENDED:
(List to be provided later)
MVI-95: PLANT VIRUSES AND DISEASES
(3 CREDIT HOURS)

Plant Viruses and their nomenclature

Sugar Cane Viruses

Woody plant virus

Cotton Curl Leaf virus

Other Cash crop viruses

Delivery of foreign genes into plants (Agro bacterium Ti based vs. shotgun approaches)

Plant viruses life cycle

Host interactions

Spread through insect vectors

Possible Antiviral approaches for Plant viruses

Viroids, Virosoids.

RECOMMENDED BOOKS:

Interaction between plant viruses and their vectors by R T Plumb.

Plant viruses and viral diseases volume XIII by Frans.

Identification of plant viruses methods and experiments by Nordun.

Atlas for insects and insects plant viruses by Karl.
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