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Bugs, Thugs and the
Law
Insects as tools in legal
investigations
Dr. David Rivers
Department of Biology
Loyola University Maryland
My teaching
Hired to teach:
Other courses taught
 Forensic Entomology
 Forensic Biology
 Organismal Biology
1)
2)
3)
4)
5)
Invertebrate Zoology
Comparative Physiology
General Genetics
Intro Anatomy and Physiology
General Entomology
 Process of Science
 Exploring Ecosystems
 Insects in Our World
 Seasonal Adaptations of
Animals
 Research in Biology
 Honors Research
Forensic Science
 All things ‘forensic’ are on the rise, especially in terms
of interest by college students
 Television shows like CSI, NCIS, Law and Order and
others have had a major impact, good and bad. The
CSI effect
 The ‘science’ is now being mandated to actually be
science, if to be used in US judicial system
 Forensic Entomology has been a beneficiary of all the
attention
Forensic
Entomology
What is it?
Brief: The broad field were arthropod
science and the judicial system
interact.
In depth: The branch of forensic
science in which information about
insects is used to draw conclusions
when investigating legal cases relating
to both humans and wildlife, although
on occasion the term may be
expanded to include other arthropods.
Plan of Attack
Forensic Entomology
What is it really?
Medicocriminal Entomology
What most people think of
Application of Insects to Criminal Investigations
Bugs, Thugs, and the Law
Forensic
Entomology
What is it
really?
Forensic Entomology
Three recognized sub-fields:
1) Medico-criminal: Focuses on the criminal
component of the legal system that deals
with necrophagous or carrion insects that
infest and feed on human remains and
carrion
2) Urban: Deals with insects affecting man
and immediate environment.
3) Stored Product: Focused on insects found
in foodstuffs.
Urban Entomology
Stored Product
Entomology
 Insects in human habitation
 Insects in food and food
 Includes those that are
 Defect action levels must be
synanthropic and some rural
species
products
exceeded
-both are subfields of General Entomology as well as Forensic Entomology
Medicocriminal
Entomology
What most
people think of
Medico-criminal Entomology
This is what most people think of when
hearing term Forensic Entomology
Why?
 Bugs on bodies are what is shown on crime shows,
Dirty Jobs, and in news articles
 It is gross in a fascinating way
 Solving murders with maggots captures the
imagination
Medico-criminal Entomology
What can insects reveal about a crime,
especially a suspicious death or homicide?
Two important pieces of information:
1) Estimation of the post mortem interval based
on development.
2) Whether the body has been moved to
its present location.
Characteristics of useful insects as
physical evidence
° necrophagous on humans or other
animal remains
° feed on necrophagous species
° development tied to corpse/carrion
° developmental details under wide
range of conditions are known
° are poikilothermic
Necrophagous Diptera
The most important ecological evidence at a
crime scene
Why flies?
 Larvae of most species from the families Calliphoridae
and Sarcophagidae are entirely
saprophagous/necrophagous
 Flies and corpse are poikilothermic
 Timing of colonization and succession relatively
predictable. Blow flies are generally first colonizers
 Most need protein to lay eggs, although some do not
need to eat before laying eggs or larvae
 Display determinant growth
Calliphora vomitoria
Family Calliphoridae
Chrysomya rufifacies
Family Calliphoridae
Sarcophaga bullata
Family Sarcophagidae
Musca domestica
Family Muscidae
Important Forensic Flies
 Calliphoridae: blow flies and bottle flies
 Sarcophagidae: flesh flies
 Muscidae: house fly, face fly, dump fly
 Fanniidae: lesser house fly
 Phoridae: scuttle fly, coffin fly
 Piophilidae: skipper flies
 Scathophagidae: dung flies
 Stratiomyidae: soldier flies
 Psychodidae: moth and sand flies
Why not beetles?
 Necrophagous Coleoptera are generally the
second most important insects on carrion in
terms of Forensic Entomology
Their utility is limited because:
1) Neither adults or larvae are exclusively necrophagous
2) Development of many species is indeterminant
3) Are more variable in terms of timing of succession
Necrophilia americana
Siliphidae
Oiceoptoma noveboracens
Siliphidae
Creophilus maxillosus
Staphylinidae
Dermestes maculatus
Dermestidae
Application of Insects to
Criminal Investigations
Bugs, Thugs,
and the Law
The ‘Crimes”
1)Suspicious deaths and homicides: estimations of PMI and
location of body
1)Abuse and neglect: myiasis
1)Pet abuse and neglect
1)Wildlife poaching
1)Toxicology: analysis of larvae and puparia
1)Blood spatter analysis: insect artifacts
1)Terrorism: use as weapons and as tools
Insects and Death
Insects and Death
 Necrophagous fly larvae are most useful
 Oldest larvae on corpse is collected
 Some are preserved as physical evidence
 Others are reared to adult for identification
 Developmental duration is estimated based on degreeday models, developmental threshold, and
experimental data
 This developmental estimate is used to estimate the
minimum post mortem interval
Factors Influencing
Development on Corpse
 Ambient temperature
 Maggot mass temperature
 Tissue composition and size
 Conspecific and allospecific competition
 Clothing or other materials covering body
 Sun vs. shade
 Seasonality
 Concealment in artificial or natural location
 Cause and manner of death
Casey Anthony Murder Trial
 Case hinged on entomological evidence, mainly the scuttle fly
Megaselia scalaris
Entomotoxicology
 Concerned with detection of drugs or toxins in
necrophagous insects from corpse
 Also on impact of exogenous chemicals on development of
forensic indicator species
 Includes detection of gunshot residues and other explosive
materials
 Fly larvae from early colonizing species are most useful
 Those feeding on surface tissues are best for GSR detection
Blood Spatter
Fly Artifacts
Fly Artifacts
 Insects are not helping with criminal investigation
 Spots or artifacts contaminate bloodstain evidence and
confuse what is real versus what is not
 Artifacts originate from regurgitate and liquid frass
(feces)
Fly spots
Blood spatter
Entomological Terrorism
 The idea of using insects as weapons is not ‘new’
 However, today’s more sophisticated use does rely on
new technologies and opportunities
Threats to US are in three forms:
1) Direct attack by biting and stinging insects
2) Use of insects as vectors of disease
3) Targeting agriculture
Forensic topics allow
discussions of:
 Succession
 Competition
 Predation and parasitism
 Environmental influences and adaptation
 Growth and development
 Introduction of modeling and statistics,
especially with degree-day concepts and post
mortem interval calculations
 Small insect collections but that require a lot
of information
Additional Information:
 Byrd, J.H. and J.L. Castner. 2010. Forensic Entomology: The
Utility of Arthropods in Legal Investigations. CRC Press, Boca
Raton, FL.
 Rivers, D.B. and G.A. Dahlem. 2014. The Science of Forensic
Entomology. Wiley-Blackwell Publishers, West Sussex, U.K.
Career Paths to ‘Forensics’
Forensic Studies
Forensic Science
 Minimally a college degree is
 Minimally a college degree is
required, usually graduate
training is needed
 Majors: Psychology,
Sociology,
Criminology/Criminal Justice
 Employers: Law
enforcement, government,
academia
required, at least a master’s
degree
 Majors: Biology, Chemistry,
Computer Science, Physics,
Mathematics
 Employers: Crime labs,
government and private
industry, academia
Majors in red are high demand for employers with those underline being priority
areas by the FBI, NSA, and Homeland Security
Career resources
 American Academy of Forensic Sciences:
http://www.aafs.org
 Loyola University’s Forensic Studies:
http://www.loyola.edu/academic/forensicstudies/careeropportunities
Questions?