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Catalog #: 0801032SP HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 (HIV-1) Infectious Sucrose Purified Virus PRODUCT DESCRIPTION: ETIOLOGIC STATUS/BIOHAZARD TESTING: Human Immunodeficiency Virus Type 1 (HIV-1) is an enveloped retrovirus approximately 120 nm in diameter, which contains two copies of a singlestranded, positive-sense RNA. HIV-1 is a Biosafety Level 2 organism, but is considered Biosafety Level 3 in large volumes. HIV-1 (Strain: IIIB) is propagated in the Hut78 T cell line. Sucrose purified HIV-1 is sold in 1.0 mg aliquots, and is shipped on dry ice. The virus from culture fluid is ultra-centrifuged, resuspended in PBS, and then purified through a one-step sucrose gradient. The viral band is collected, ultra-centrifuged, and the pellet is resuspended in PBS. Each lot of sucrose purified virus is assayed for its total protein concentration. Tissue Culture Infective Dose (TCID50) or RT-PCR value may be available upon request. Custom orders are available, including specific titers and package sizes. INTENDED USE: This product is intended for research, product development testing, or quality assurance testing. Sucrose purified viruses are sold as consumable testing materials, and are not for propagation or commercialization. Applications include: Nucleic Acid / Molecular Testing Limit of Detection (LOD) Studies Cross-reactivity Studies Other Viral-based Assays PRECAUTIONS: USE UNIVERSAL PRECAUTIONS when handling this product! Sucrose purified viruses are live and infectious!! This material should be handled as if capable of transmitting infectious agents. RECOMMENDED STORAGE: Sucrose purified viruses are stable for at least one year when stored at -65ºC or below. To avoid repeat freeze-thaws, which could negatively impact product performance, sucrose purified viruses should be stored in aliquots upon receipt. DO NOT USE IN HUMANS! These products are NOT intended for use in the manufacture or processing of injectable products subject to licensure under section 351 of the Public Health Service Act, or for any other product intended for administration to humans. The purchase of live organisms from ZeptoMetrix requires a Material Transfer Agreement (MTA). This product was manufactured in a facility which has a Quality Management System that is ISO 13485 certified. PI0801032SP Rev. No./Replaces: 0 / New Research Use Only ZeptoMetrix Corporation 872 Main Street Buffalo, New York 14202 Office Phone: 716-882-0920 Fax: 716-882-0959 Customer Service: 800-274-5487 E-mail: [email protected] www.zeptometrix.com PCA No.: 13-080