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ULM School of Health Professions Spring 2017 COURSE TITLE: MLSC 3030, Medical Microbiology I I. Contact Information Instructor: Jessica Lasiter, MLS (ASCP)CM Phone: 318.342.1631 Email: [email protected] Webpage: www.ulm.edu/mls Office: Sugar 253 Office Hours: 1:00 p.m. - 3:00 p.m. Monday – Wednesday 7:30 a.m. - 11:30 a.m. Friday Other hours available by appointment Preferred mode of communication with instructor: email I. Course Netiquette Expectations II. Always think before you write. So please think twice before you hit submit. Keep it relevant. There are places to chat and post for fun everyday stuff. Do not stray from the discussion in the assigned questions. Never use all caps. This is the equivalent of yelling in the online world. It is not fun to read. Only use capital letters when appropriate. Make sure that you are using appropriate grammar and structure. In other words, I don’t want to see anyone writing “R U” instead of “are you”. There are people in the class that may not understand this type of abbreviation, not to mention it does nothing to help expand your writing and vocabulary skills. Emoticons are fine as long as they are appropriate. A smile is welcome, anything offensive is not. Treat people the same as you would face-to-face. In other words, it is easy to hide behind the computer. In some cases, it empowers people to treat others in ways they would not in person. Remember there is a person behind the name on your screen. Treat all with dignity and respect and you can expect that in return. Email Policy Various services on campus require official “warhawks” student email, and will not accept a third party account such as Yahoo or Google. The myULM portal (http://my.ulm.edu) is the best way to receive important notices and to know about activities on campus. Warhawks.ulm.edu (students) and ulm.edu (faculty) are considered the official university email address. When conducting university business through email, please use only a student’s warhawks.ulm.edu account and/or faculty’s ulm.edu account. II. Course Description Lasiter MLSC 3030:1701 Page 1 ULM School of Health Professions Spring 2017 4 cr. The morphology, physiology, and immunogenicity of disease-causing bacteria, methods of isolation, identification, susceptibility testing, and control of pathogenic bacteria and diseases. Three hours lecture and one hour laboratory. III. Course Prerequisites/Corequisites MLSC majors only IV. Course Objectives (Lecture) A. Define these terms or phrases: microbe, bacteria, virus, parasite, fungus, prion, rickettsia, virulence, virulence factors, mode of transmission. B. Discuss the major events in the history of medical microbiology. C. State the Gram staining reaction, shape, and virulence factors of the major bacterial pathogens of man. D. State the principle of the major diagnostic test in the microbiology laboratory. E. List the various health hazards inherent in the microbiology laboratory and discuss how they can be minimized. F. List the steps in the Gram staining procedure and state the principle. G. Define “antibiotic resistance” and discuss how this can occur in the microbial world. H. Define “sensitivity testing” and describe the various methods to perform this analysis. I. Describe the proper way to obtain isolated bacterial colonies. J. List the major types of culture media and state the utility of each. K. Describe the proper way to “read culture plates.” L. Describe the performance and interpretation of miniaturized biochemical test batteries such as the Analytical Profile Index. Course Objectives (Laboratory) A. Perform a “streak plate” procedure to obtain isolated bacterial colonies. B. Evaluate a culture plate containing bacterial growth and determine which colonies should be worked up for identification and antibiotic sensitivity studies. C. Examine a Gram stain smear and identify the Gram Stain reaction and morphology of the bacteria. D. Perform an antibiotic sensitivity procedure on an isolated bacterial colony to determine which antibiotics are effective against the organism. E. Perform a Gram stain on a bacterial smear to determine its Gram stain reaction and shape. F. Demonstrate the correct biochemical methods for identifying pathogens G. Demonstrate setting up an Analytical Profile Index procedure to identify bacteria biochemically. H. Perform acid-fast bacilli stains. * Detailed objectives will be provided for each lecture. V. Course Topics Introduction to Medical Microbiology, The Gram Positive Cocci, Neisseria (Gram Negative Diplococci), Haemophilus (Tiny Gram Negative Bacilli), Enterobacteriaceae (Fermentative Gram Negative Bacilli), Nonfermentative Gram Negative Bacilli, Miscellaneous Gram Negative Bacilli, Aerobic Gram-Positive Non- Lasiter MLSC 3030:1701 Page 2 ULM School of Health Professions Spring 2017 Spore Forming Bacilli, The Aerobic Gram-Positive Spore-Forming Bacilli (Bacillus Genus), Anaerobic bacteria, Spirochetes and Curved Rods, Mycobacteria, Rickettsia, Mycoplasma and Ureaplasma VI. Instructional Methods and Activities The instructional methods and activities of this course will include lecture, video presentation of pertinent concepts, slide presentations, case history studies, class discussion, objective examinations, pop tests, practical examinations and notebook construction. Oral presentations, written critiques of video presentations, and research projects may also be used. VII. Evaluation and Grade Assignment A. Lecture Grade Average The lecture grade average will be determined by the student’s scores on scheduled chapter examinations, pop test results, student projects and the final examination (comprehensive). There will be approximately five comprehensive lecture examinations and the final comprehensive examination. The lecture grade average comprises 75% of the final course grade. The final exam is comprehensive and counts as 20% of the lecture grade average. Laboratory Grade Average The laboratory grade average is derived from the laboratory journal grade, quizzes and practicals and counts as 20% of the final course grade. The total points earned divided by the total points possible will be used to calculate the laboratory grade average. The final laboratory exam is comprehensive and counts as 20% of the laboratory grade average. Beginning with Week 2, a quiz on the previous week's lab procedures and lectures will be given at the beginning of the class period, followed by a lecture on the laboratory procedure for that week. Preparing for the lab exercise by reviewing the reading assignment before each lecture will help you have a better understanding of the material presented. Each procedure performed in the laboratory will be documented in a laboratory journal. Instructions for the journal are supplied on a separate document. Practical examination will be given throughout the semester. In order to progress in this course, a 75% on each practical must be achieved. Affective Grade Average An affective evaluation shall determine 5% of the final grade. The student begins with 100% for the affective evaluation. 10 points are subtracted for each violation of proper behavior. B. Grading Scale A = 93%–100% B = 84%–92% C = 76%-83% Lasiter MLSC 3030:1701 D = 70%-75% F = 69% and below Page 3 ULM School of Health Professions Spring 2017 C. Undergraduate mid-term grades will be posted on-line for students to view via Banner. Mid-term grades indicate the student’s status at mid-semester only and do not indicate the final performance outcome of the student. D. Students MUST earn at least a C in all medical laboratory science courses to progress in the professional program. A non-progressive grade of D will necessitate remediation of the course according to the MLS professional program’s remediation policy. A failing grade of F will result in suspension from the professional program. E. Students must complete course work in those courses where “I” grades have been earned within the first month of the following semester or within the first two weeks of a summer term in which they resume studies at ULM or elsewhere. The student has one calendar year to make arrangements to have the “I” grade removed. If the “I” grade is not removed in the specified time, the “I” is calculated as an “F” into the cumulative grade point average by The University of Louisiana at Monroe Registrar. VIII. Class Policies and Procedures A. Textbook(s) and Materials Required Kiser, K. M., Payne, W. C., Taff, T. A. (2011). Clinical Laboratory Microbiology: A Practical Approach. Boston: Pearson. ISBN 13: 978-0013-092195-6 University of Louisiana at Monroe Medical Laboratory Science Student Handbook Speckled Journal Colored Pencils Pencil Sharpie Lab Coat B. Attendance Policy: Class attendance is regarded as an obligation as well as a privilege, and all students are expected to know attendance regulations and to attend regularly and punctually all classes in which they are enrolled. Failure to do so may jeopardize a student’s scholastic standing and may lead to suspension from the university. Attendance records are subject to inspection by appropriate college or university officials. C. Make-up Policy Due to the format of this course, all assignments and projects are due on the stated date and will not be accepted late. If the student has an excusable reason for not attending class, proper documentation must be provided to the instructor. Lasiter MLSC 3030:1701 Page 4 ULM School of Health Professions Spring 2017 Documentation is mandatory! Students will follow the policy outlined in the current Undergraduate Catalog and the Student Handbook. D. Academic integrity Faculty and students must observe the ULM published policy on Academic Dishonesty (see current copy of ULM Student Policy Manual http://www.ulm.edu/studentpolicy/). E. Course Evaluation Policy Students are expected to complete the course evaluation available on-line and the clinical instructor(s) evaluation and clinical facility/department evaluation forms in the Student Handbook. F. Student Services The University of Louisiana at Monroe strives to serve students with special needs through compliance with Sections 504 of the Rehabilitation Act of 1973 and the Americans with Disabilities Act. These laws mandate that postsecondary institutions provide equal access to programs and services for students with disabilities without creating changes to the essential elements of the curriculum. While students with special needs are expected to meet our institution's academic standards, they are g iven the opportunity to fulfill learner outcomes in alternative ways. Examples of accommodations may include, but are not limited to, testing accommodations (oral testing, extended time for exams), interpreters, relocation of inaccessible classrooms, permission to audiotape lectures, note-taking assistance, and course substitutions. Information about ULM student services can be found via these links: Student Success Center: http://www.ulm.edu/studentsuccess/ Counseling Center http://www.ulm.edu/counselingcenter/ Special Needs at http://www.ulm.edu/studentaffairs/ Library http://www.ulm.edu/library/reference.html Computing Center Help Desk http://www.ulm.edu/computingcenter/helpdesk Current college’s policies on serving students with disabilities can be obtained on the ULM website: http://ulm.edu/counselingcenter/ If you need accommodation because of a known or suspected disability, you should contact the Director for Disabled Student Services at: Voice phone: 318-342-5220 Fax: 318-342-5228 Lasiter MLSC 3030:1701 Page 5 ULM School of Health Professions Spring 2017 Walk-In: ULM Counseling Center, 1140 University Avenue (this building and room are handicapped accessible). Mental Wellness on the ULM Campus If you are having any emotional, behavioral, or social problems and would like to talk with a caring, concerned professional, please call one of the following numbers: The ULM Counseling Center: 318-342-5220 The Marriage and Family Therapy Clinic: 318- 342-9797 The Community Counseling Center: 318-342-1263 Remember that all services are offered free to students, and all are strictly confidential. If you have special needs that I need to be made aware of, you should contact me within the first two days of class. Sexual Harassment or Gender-Based Discrimination Title IX of the Education Amendments of 1972 prohibits sex discrimination against any participant in an educational program or activity that receives federal funds, including federal loans and grants. Furthermore, Title IX prohibits sex discrimination to include sexual misconduct, sexual violence, sexual harassment and retaliation. If you encounter sexual harassment or gender-based discrimination, please contact the Title IX Coordinator at 318-342-1004; you may also file a complaint online, 24 hours a day, at: www.ulm.edu/titleix. G. Emergency Procedures Students will follow the emergency procedures of the facility in which the practicum is completed. University Police Phone: 318.342.5350 In case of an emergency, dial 911 H. Discipline/Course Specific Policies See Student Handbook for affective objectives and evaluation form, attendance policy, grading policy and discipline policy. Tardiness is unacceptable. Each student is responsible for arriving to class on time. Each tardy will be documented, and three tardies by a student will convert into one unexcused absence. It is the student’s responsibility to inform the instructor of extenuating circumstances that caused the student to be tardy. Cell phones and pagers must be turned off or silenced during class. If there is an emergency, the student must leave the classroom and not disrupt the class or instructor. Violation of this expectation may result in the student’s grade being lowered. Lasiter MLSC 3030:1701 Page 6 ULM School of Health Professions Spring 2017 Class disruption: When the instructor begins the lecture, all talking MUST cease. Students who disrupt class will be asked to leave. Chronic classroom disruption will result in the student being removed from the class roster. IX Tentative Course Schedule Instructor: Jessica Lasiter, MLS (ASCP)CM Phone: 318.342.1631 Email: [email protected] Webpage: www.ulm.edu/mls Office: Sugar 253 Office Hours: 1:00 p.m. - 3:00 p.m. Monday – Wednesday 7:30 a.m. - 11:30 a.m. Friday Other hours available by appointment Lasiter MLSC 3030:1701 Page 7 ULM School of Health Professions Date Spring 2017 MICROBIOLOGY LECTURE TENTATIVE SCHEDULE Day Subject Reading 17-Jan Tu 19-Jan 24-Jan 26-Jan 31-Jan 2-Feb 7-Feb 9-Feb 14-Feb 16-Feb 21-Feb 23-Feb 28-Feb 2-Mar Th Tu Th Tu Th Tu Th Tu Th Tu Th Tu Th 7-Mar Tu 9-Mar 14-Mar 16-Mar 21-Mar 23-Mar 28-Mar 30-Mar 4-Apr 6-Apr 11-Apr 13-Apr 18-Apr 20-Apr 25-Apr 27-Apr 2-May 4-May Th Tu Th Tu Th Tu Th Tu Th Tu Th Tu Th Tu Th Tu Th Intro, Taxonomy, Bacterial Cell, Host Encounter & Safety Specimen Collection & Cultivation Presumptive Identification Final Identification Exam I Gram Positive Cocci Gram Positive Cocci cont. Gram Negative Cocci Gram Positive Rod Fastidious Gram Negative Rod Exam II Enterobacteriacea Mardi Gras Break Enterobacteriacea cont. Nonfermenter Gram Negative Rods, Vibrio, Aeromonas, & etc. Vibrio, Aeromonas, Plesiomonas, etc. Exam III Anaerobes Spirochetes Miscellaneous Microorganisms Mycoplasma and Ureaplasma Exam IV Chapters 1-5 Chapter 6 & 7 Chapter 8 Chapter 9 Chapter 17 Chapter 17 Chapter 18 Chapter 19 Chapter 20 Chapter 21 Chapter 21 Chapter 22 Chapter 23 Chapter 24 Chapter 25 Chapter 27 Chapter 28 LSCLS Spring Meeting Susceptibility Testing Susceptibility Testing Chapter 11 Chapter 11 Spring Break Immunologic; Emerging Technologies Mycobacterium species Global Threats Exam V Chapter 10 & 12 Chapter 26 Chapter 42 Final Exam – May 9, 2017 @ 10am **The instructor reserves the right to adjust the schedule as needed. Lasiter MLSC 3030:1701 Page 8 ULM School of Health Professions Spring 2017 MICROBIOLOGY LABORATORY TENTATIVE SCHEDULE Date Day Procedures 17-Jan 19-Jan 24-Jan 26-Jan Tu Th Tu Th 31-Jan Tu 2-Feb 7-Feb 9-Feb 14-Feb 16-Feb 21-Feb 23-Feb 28-Feb 2-Mar Th Tu Th Tu Th Tu Th Tu Th 7-Mar Tu 9-Mar Th 14-Mar 16-Mar 21-Mar 23-Mar 28-Mar 30-Mar 4-Apr 6-Apr 11-Apr 13-Apr 18-Apr 20-Apr 25-Apr 27-Apr 2-May 4-May Gram Stains St. Francis Medical Center Lecture Gram Stains Gram Stain Practical Staphylococcus/Streptococcus (Gram Stains, Catalase, Coagulase, Bile Solubility, PYR) Staphylococcus/Streptococcus (BE, CAMP, NaCL, Taxo A & P) Gram Positive Rods (Gram stains, Hemolysis, motility, TSI) Gram Negative Cocci (Gram Stains, oxidase, butyrate esterase) Fastidious Gram Negative Rods (X & V Factors and Gram stains) Fastidious Gram Negative Rods (X & V Factors and Gram stains) Unknown # 1 Practical (GPC, GPR, GNC, Fast GNR) Unknown # 1 Practical (GPC, GPR, GNC, Fast GNR) Mardi Gras Break Study Day Enterobacteriaceae (Gram Stains, Urea, Citrate, ODC/LDC, PAD, TSI, oxidase, IMViC, Motility, etc.) Enterobacteriaceae (Gram Stains, Urea, Citrate, ODC/LDC, PAD, TSI, oxidase, IMViC, Motility, etc.) Unknown # 2 Practical (Enterobacteriaceae) Unknown # 2 Practical (Enterobacteriaceae) Unknown #3 Practical(mixed cultures) Unknown # 3 Practical(mixed cultures) Unknown # 4 Practical(mixed cultures) Unknown # 4 Practical(mixed cultures) Tu Th Tu Th Tu Th Tu LSCLS Spring Meeting Th Tu API Strips & Anaerobic ATP Th Susceptibility Testing Tu Spring Break Th Tu Susceptibility Testing Th Kinyoun Stains Tu Final Practical (mixed cultures) Th Final Practical (mixed cultures) *Must earn a 76% on all Practical Assignments to progress in course. **The instructor reserves the right to adjust the schedule as needed. Lasiter MLSC 3030:1701 Page 9 ULM School of Health Professions Spring 2017 MLSC 3030 Medical Microbiology Affective Evaluation An affective evaluation shall determine 5% of the grade. The student begins with a 100% for the affective evaluation. 10 points are subtracted for each violation of proper behavior. Violations include, but are not limited to, the following: 1. Wasting time or loitering in class or failure to participate 2. Use of any personal electronic device including cell phones, I-Pods, radios, etc. 3. Carelessness or inefficient work/school habits 4. Creating or contributing to unsanitary or untidy conditions (includes failure to keep classroom and laboratory tidy and orderly) 5. Improper use or care of university property including laboratory equipment 6. Distracting behavior during instructional activities including, but not limited to, talking out of turn or passing notes 7. Horseplay, practical jokes, etc. 8. Unexcused Absence 9. Tardiness 10. Lack of respect for faculty and classmates through words, actions, or intent. An affective score below 75% shall result in failure of the course. Lasiter MLSC 3030:1701 Page 10 ULM School of Health Professions Spring 2017 I verify that I have received a copy of the course syllabus for Medical Laboratory Science 3030, Medical Microbiology. This entire document has been reviewed with me by the instructor of the class. ___________________________________ Student Name (PRINTED) ___________________________________ Student Signature ___________________________________ Date ___________________________________ Current (Local) Address ___________________________________ City/State/Zip ___________________________________ Current (Local) Phone Number ___________________________________ Email Address (PRINTED) Lasiter MLSC 3030:1701 Page 11