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Oxidative Effects of Boric Acid on Different Developmental Stages of Drosophila melanogaster Eda Güneş1, Ender Büyükgüzel2 1 Ahmet 2 Erdoğan Vocational School of Health Services, Bülent Ecevit University, Zonguldak Department of Biology, Faculty of Science, Bülent Ecevit University, Zonguldak [email protected] Abstract Synthetic organic insecticides are widely used to combat agricultural pests. Boric acid is a great importance in pest management because it has less toxic effect on non-target organisms according to other organic chemical insecticides. For this purpose, the insect was reared from first-instar larvae on an artificial diets containing boric acid at 10, 100, 200 or 300 mg (mg/L) to adult stage. The effect of boric acid on important oxidative stress indicators; lipid peroxidation product, malondialdehyde (MDA) and protein oxidation products, protein carbonyl (PCO) contents and detoxification enzyme, glutathion-S-transferase activity in the 3rd larval instars, pupae, adults and eggs of the fruit fly Drosophila melanogaster were investigated. All boric acid concentrations significantly increased MDA content in third larval stage. When the adults from the larvae reared on 300 mg of dietary BA were also fed with 300 mg/L BA concentration for a 10-day period, MDA and PCO contents of male and female adults were significantly increased in comparison to control. MDA and PCO content in the eggs of these females were significantly increased. The increase in PCO content of the eggs was 31-fold relative to control. Our results indicate that BA feding at high concentrations in all developmental stage of D. melanogaster is more effective on oxidative stress indicators and detoxification enzyme. Keywords: Boric acid, Drosophila melanogaster, Oxidative Stress
