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THE VICTORIA SCHOOL TEACHER: DIANA ANGÉLICA CARVAJAL BERNAL LAB: HEART DISSECTION MAY 8th/10TH 2012 BIOLOGY 8TH GRADE MYP YEAR 4TH Criteria Evaluated E: Processing Data B: Communication in Science AIMS: Identify and compare the size, shape and tissue type of the mayor anatomic landmarks of the heart. Associate the heart’s structure with its function. 1. DATA COLLECTION: This lab practical allows you to identify and compare the size, shape and tissue type of the major chambers and vessels of the heart. The goal of the lab is not, however, just to observe anatomy, but to associate structure with function. The heart is a pump for blood that comes into the right atrium, goes out to the lungs through the right ventricle, returns through the left atrium, and leaves again through the left ventricle. Each chamber is separated by valves that prevent the backflow of blood. Try and figure out where the various components are, how each works, especially how the shape, composition, and even texture of each part contribute to its function. Materials Dissection kit Rubber/latex gloves Pig Heart Drawing pencils and/or Digital Camera Dissection guide and results table Diagram of heart Observation: External Anatomy — DO NOT CUT ANYTHING YET! As you follow the instructions and find each structure, label a pin and stick the pin in the structure. You must see all 10 structures before you may continue to the internal structures. 1. Identify the right and left sides of the heart. Look closely and on one side you will see a diagonal line of blood vessels that divide the heart. The half that includes all of the apex (pointed end) of the heart is the left side. Confirm this by squeezing each half of the heart. The left half will feel much firmer and more muscular than the right side. (The left side of the heart is stronger because it has to pump blood to the whole body. The right side only pumps blood to the lungs). 2. Turn the heart so that the right side is on your right, as if it were in your body. Find the large opening at the top of the heart next to the right auricle (flap of darker tissue on top of the heart). This is the opening to the superior vena cava, which brings blood from the top half of the body (arms and head) to the right atrium. Carefully stick a glass rod down this vessel. You should feel it open into the right atrium. A little down and to the left of the superior vena cava there is another blood vessel opening. This is the inferior vena cava, which also leads to the right atrium, bringing blood from the lower tissues (legs and abdomen). You can also see another blood vessel next to the left auricle. This is a pulmonary vein that brings blood from the lungs into the left atrium. 3. Sticking straight up from the centre of the heart is the largest blood vessel you will see. This is the aorta, which takes oxygenated blood from the left ventricle to the rest of the body (the ventricles are the lower chambers of the heart). The aorta branches into more than one artery right after it leaves the heart, so it may have more than one opening on your heart specimen. Look carefully at the openings and you should be able to see that they are connected to each other. 4. Behind and to the left of the aorta there is another large vessel. This is the pulmonary artery which takes blood from the right ventricle to the lungs. Before you cut anything, draw or take a picture of a simple, coloured, front (ventral) and a back (dorsal) external view of the heart. This one will go in the lab report. Dissection: Internal Anatomy As you work, make observations, drawings, photos and measurements of as many structures as you can, filling in the chart. 1. Insert your dissecting scissors or scalpel into the superior vena cava and make an incision down through the wall of the right atrium and right ventricle. Pull the two sides apart and look for three flaps of membrane. These membranes form the tricuspid valve between the right atrium and the right ventricle. The membranes are connected to flaps of muscle called the papillary muscles by tendons called the chordae tendinae or "heartstrings." This valve allows blood to enter the ventricle from the atrium, but prevents backflow from the ventricle into the atrium. Aorta The two vena cava go into the right atrium on the other (dorsal) side Pulmonary artery The pulmonary vein goes into the left atrium on the dorsal side. Coronary artery and vein When you need to see inside the right ventricle, cut here. When you want to open the left ventricle cut here. 2. Insert a glass rod into the pulmonary artery and see it come through to the right ventricle. Make an incision down through this artery and look inside it for three small membranous pockets. These form the pulmonary semilunar valve which prevents blood from flowing back into the right ventricle. 3. Insert your dissecting scissors or scalpel into the left auricle at the base of the aorta and make an incision down through the wall of the left atrium and ventricle, as shown by the dotted line in the external heart picture. Locate the mitral valve (or bicuspid valve) between the left atrium and ventricle. This will have two flaps of membrane connected to papillary muscles by tendons. 4. Insert a glass rod into the aorta and observe where it connects to the left ventricle. Make an incision up through the aorta and examine the inside carefully for three small membranous pockets. These form the aortic semilunar valve which prevents blood from flowing back into the left ventricle. In drawings or photos, show all the parts of the heart that are in black. Include this in the lab report. 2. PROCESSING AND PRESENTING DATA: Include text and drawings/photos of the steps of the dissection. In the photos, label the structures of interest. Each drawing/photo must have a caption (explanation). Also include in this section your completed tables of measurements and observations. Photos must be clear and well presented, otherwise drawings are necessary. Structure Diameter (mm) Wall Thickness (mm) Observations (color, texture, etc.) Ahorta Right Atrium Left Atrium Vena Cava Right Ventricle Left Ventricle Pulmonary Artery Pulmonary Vein 3. CONCLUSION AND EVALUATION: - Include two to four WELL WRITTEN conclusions that articulate the theory with the results found in the lab practice. - Evaluate procedures and results including limitations, weaknesses or errors. - Identify weakness and states realistic suggestion to improve the investigation. Suggest future experiments. 4. BIBLIOGRAPHY