Download Tipvadee Attathoma1 c1, Wiboon Chongrattanameteekula1, Jariya

ScienceAsia 36(1): 6-11. 2010
Laboratory studies on control of the maize weevil Sitophilus zeamais by the parasitoid
Anisopteromalus calandrae
Chaisaeng, P.a , Chongrattanameteekul, W.b , Visarathanonth, P.c , Vajarasathiara, B.d
a
Company Limited, Bangplad, Bangkok 10700, Thailand
Department of Entomology, Faculty of Agriculture, Kasetsart University, Bangkok 10900,
Thailand
c
Postharvest Technology Research and Development Group, Postharvest and Product
Processing Research and Development Office, Department of Agriculture, Bangkok 10900,
Thailand
d
Department of Zoology, Faculty of Science, Kasetsart University, Bangkok 10900,
Thailand
b
Abstract
Anisopteromalus calandrae (Howard), a cosmopolitan parasitoid, attacks several beetle pests
of stored products, including the maize weevil, Sitophilus zeamais (Motschulsky). Two
laboratory experiments were conducted at ambient conditions (25-29 °C, 60-70% RH, natural
photoperiod). A short-term experiment evaluated the density of A. calandrae (0, 4, 8, 12, 16,
or 20 per box) for the optimal production of parasitoids in 3825-cm 3 boxes containing milled
rice. The percentage of parasitoid emergence was highest and the percentages of weevil
emergence and parasitoid-induced mortality i.e., parasitoid mortality resulting from
superparasitism were relatively low with 16 females per box rather than with smaller (0 to
12) or larger (20) numbers of females added per box. The decline in parasitoid emergence at
the highest parasitoid density tested (20 females per box) was probably due to
superparasitism. A long-term experiment was conducted to determine the parasitoid densities
(0, 2, 4, 6, 8, or 10 per bottle) that most effectively suppress weevils in milled rice during 6
months of storage in 1-1 bottles. To simulate repetitive release of parasitoid, the same number
of parasitoids was introduced after each monthly sampling. The number of maize weevils
decreased as parasitoid density increased. The best control was obtained with 10 female
parasitoids per bottle. At this density, the number of emerged weevil remained stable from
the third to the sixth month. Based on the short-term experiment, a parasitoid-host ratio of
1:47 will produce the largest number of parasitoids. Based on the long-term experiment, a
parasitoid-host ratio of 1:30 will provide the best control of maize weevils in a monthly
release program. The current results indicate that large-scale experiments on biological
control of the maize weevil with A. calandrae are now needed.
Keywords: Biological control; Ectoparasitoid; Induced mortality; Repetitive release
Kasetsart Journal - Natural Science 43(2):261-268. 2009
Biological control of the coconut hispine beetle, brontispa longissima gestro (coleoptera:
Chrysomelidae) by the parasitoid, asecodes hispinarum bouček (Hymenoptera:
Eulophidae) on a golf course
Pundee, T.a , Jamornmarn, S.a , Chongrattanameteekul, W.a , Sindhusake, C.b , Winotai,
A.b , Morakote, R.b
a
Department of Entomology, Faculty of Agriculture, Kasetsart University, Bangkok 10900,
Thailand
b
Plant Protection Research and Development Office, Department of Agriculture, Bangkok
10900, Thailand
Abstract
The coconut hispine beetle, Brontispa longissima Gestro is an invasive pest in Southeast
Asia. Since chemical control of the beetle is not recommended due to its high cost and risk to
the environment, biological control is a desirable tool for effective control of this pest. This
experiment was conducted at the Panya Indra golf course, Bangkok in 2007 using the
parasitoid, Asecodes hispinarum Bouček, an effective larval parasitoid. The number of
parasitoids released was based on the density of the damaged coconut trees. Continual
releases were made in and around the area. Mummified larvae were counted once a month by
selecting 20 infested spear leaves. The parasitoids were observed five months after release.
The mummified larvae were collected in June, August and November and the percentage
parasitized was found to be 13.48%, 8.58% and 14.08%, respectively. Severe, leaf damage
levels decreased in December. The results showed that the new coconut palm leaves were
fresh with less damage. A population study of B. longissima was also undertaken by
randomly sampling 20 spear leaves. High and low population levels were observed from
March to June and from July to December, respectively
Keywords: Asecodes hispinarum Boucek; Biological control; Brontispa longissima Gestro;
Invasive specie
Thai J. Agric. Sci. 30, pp. 365-378. 1997
Proceedings of the AVNET-II Final Workshop, 1–6 September 1996, Bangkok, Thailand.
1997. 433-434. Asian Vegetable Research and Development Center Shanhua, Taiwan.
Mass rearing of diamondback moth parasitoids. Collaborative Vegetable Research in
Southeast Asia.
Keinmeesuke, P., J. Piriyapol, L. Ngamwongthum, and W. Chongrattanameteekul.
Proceedings, the Second Pacific Rim Conference on Biotechnology of Bacillus Thuringiensis
and Its Impact to the Environment , pp. 82-102.1996
Isolation, PCR identification and insecticidal activity of Bacillus thuringiensis strains in
Thailand
Attatom, T., Isanont, P., Siriyan, R., Chongrattanameteekul, W.
Bulletin of Entomological Research 85: 167-173. 1995
Morphological diversity and toxicity of delta-endotoxin produced by various strains
of Bacillus thuringiensis
Tipvadee Attathoma1 c1, Wiboon Chongrattanameteekula1, Jariya Chanpaisanga1 and
Ratchanee Siriyana1
a1
Department of Entomology, Faculty of Agriculture, Kasetsart University, Nakhon Pathom,
Thailand
Abstract
Local isolates of Bacillus thuringiensis were isolated from infected rice stemborers,
soils, rice bran and rice mill dust. Of 83 isolates serotyped by B. thuringiensis Hantisera, 13 serotypes were identified. Those serotypes were assigned to B.
thuringiensis of subspecies canadensis, galleriae, kenyae, kurstaki, neoleonensis,
aizawai, alesti, entomocidus, tochigiensis and tolworthi. Three serotypes, 3a:3c:3d,
5a:5b/21 and 17/27, were unnamed and portend to be new subspecies. Morphological
studies of delta-endotoxin or crystal protein of each subspecies showed significant
differences in size and shape. Bipyramidal crystals, spherical and cuboidal crystals
and the combinations of these structures were observed. Toxicity of sporulated
cultures and isolated crystal proteins of B. thuringiensis strains to five species of
economically important insects were determined using diet incorporation bioassays.
Extracted crystal proteins from certain B. thuringiensis subspecies were highly toxic
to some insect species. These have the potential of providing alternative strategies for
utilizing B. thuringiensis for controlling insects.
Bacillus thuringiensis Biotechnology and Environmental Benefits 1, pp. 69-86.1995
Bacillus thuringiensis isolation, identification and bioassay
Attathom, T., Chanpaisang, J., Chongrattanameteekul, W.
J. Appl. Entomol. 111, pp. 361-364.1991
Feeding behavior of Rhopalosiphum padi (L.) and Sitobion avenae (F.) (Hom.,
Aphididae) on wheat as affected by conspecific and interspecific interactions
Chongrattanameteekul, W., Foster, J.E., Shukle, R.H., Araya, J.E.
Abstract
The feeding behavior of Rhopalosiphum padi (L.) and Sitobion avenae (F.) was electronically
monitored on wheat seedlings (Triticum aestivum L. em. Thell., cv. ‘Caldwell’) infested at
various densities.
Conspecific competition caused an increase in non-probing time and a decrease in
probing/ingestion time for R. padi, but not for S. avenae when the density of aphids was
increased from 1 to 8 individuals per plant. Interspecific competition with S. avenae did not
significantly (P < 0.10) affect feeding behavior of R. padi, but the presence of R. padi on the
plant resulted in fewer probes, shorter non-probing time, and longer probing/ingestion time
for S. avenae. These data indicate that conspecific competition is more important in the
feeding behavior of R. padi than of S. avenae. Interaction between the two species appeared
to facilitate the feeding behavior of S. avenae.
Journal of Applied Entomology 111, pp. 249-253. 1991
Biological interactions between the cereal aphids Rhopalosiphum padi (L.) and Sitobion
avenae (F.) (Hom., Aphididae) on wheat
Chongrattanameteekul, W., Foster, J.E., Araya, J.E.
Abstract
Longevity and fecundity of the bird cherry-oat aphid, Rhopalosiphum padi (L.), and the
English grain aphid, Sitobion avenae (F.), reared at various densities on caged wheat plants
were observed under laboratory conditions. On heading plants, R. padi responded to
intraspecific competition by having a significantly shorter life and lower fecundity. When
infested with 4 R. padi + 4 S. avenae on the same plant, S. avenae had either no effect or a
positive effect on longevity and fecundity of R. padi. The presence of R. padi on wheat
sedlings, however, appeared to increase longevity and fecundity of S. avenae. Both species
had reduced longevity and fecundity when 16 R. padi + 16 S. avenae were placed on the
same plant. The bird cherry-oat aphid preferred lower parts of the heading plant, while the
English grain aphid preferred the plant head. The presence of the two species did not affect
the spatial distribution of each aphid species on the plant. Only when crowded by its own
species, S. avenae spread downward to avoid intraspecific competition on the wheat head.