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Regulatory Networks
Introduction to regulatory networks
by Rosalind Allen
Slide 2: Mammalian nerve, muscle and
blood cells.
Purkinje neurons. These are some of the
largest nerve cells in the human brain.
(Image copyright Annie Cavanagh, Wellcome Images)
Blood cells: colourenhanced image of a
small lymphocyte
surrounded by red
blood cells.
(Image copyright University of
Edinburgh, Wellcome
Images)
Primary human fibroblasts
(muscle cells); stained to
show the actin cytoskeleton
and nucleus.
(Image copyright Matthew Daniels /
University of Oxford, Wellcome Images)
Slide 3: Photograph of an agar plate with colonies of bacterial cells that switch
randomly between a state that makes the enzyme beta-galactosidase (blue) and
one that doesn’t (white). (Courtesy of van der Woude Lab, York University.)
Slide 4: 3D structure of a dimer of the lac repressor protein bound to its DNA
binding site. Reprinted by permission from Macmillan Publishers Ltd: Nature Structural Biology, C. E. Bell and M.
Lewis. 7, 209 - 214 (2000). Copyright (2011).
Slide 5: Statistical mechanics of binding to a macromolecule I: lattice model for
macromolecule–ligand binding. (From Phillips et al. 2009.)
Slide 6: Statistical mechanics of binding to a macromolecule II: derivation of the
formula for the probability of P being bound. (From Phillips et al. 2009.)
Slide 7: Statistical mechanics of binding to a macromolecule III: plot of pbound for
several binding energies. (From Phillips et al. 2009 fig 6.6.)
Slide 8: Schematic diagram of the lac regulatory system. (From Genes and Signals,
Ptashne and Gann 2002 Fig.1.3, Copyright Cold Spring Harbor Laboratory Press.)
Slide 9: Schematic representation of the lac regulatory system.
Slide 10: Schematic diagram of the phage lambda genetic switch (simplified
version). The cro gene results in cell lysis; the cI gene promotes lysogeny.