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Date of download: 5/5/2017 Copyright © ASME. All rights reserved. From: On Modeling Morphogenesis of the Looping Heart Following Mechanical Perturbations J Biomech Eng. 2008;130(6):061018-061018-11. doi:10.1115/1.2978990 Figure Legend: Optical coherence tomography (OCT) images of the embryonic heart showing the effects of splanchnopleure (SPL) removal. ((a)– (d)) Left lateral view; ((a′)–(d′)) ventral view; ((a″)–(d″)) cross-sectional view of the heart tube (HT) at locations indicated by dotted lines in ventral view. The white arrowheads in (b′)–(d′) mark the position of a cluster of beads used to visualize HT rotation; rotation angle θ is defined by orientation of lumen, as shown in (b″). ((a)–(a″)) Stage-10 heart with SPL intact. ((b)–(b″)) The heart with SPL removed after 3 h culture. ((c)–(c″)) The same heart after 6 h culture. Note that the bead cluster has not migrated rightward. ((d)–(d″)) The same heart after 12 h culture. The bead cluster has migrated to the ventral midline, indicating rotation of the heart. Rotation also can be seen in cross-sectional views (MY=myocardium and CJ=cardiac jelly). The lines in (b)–(d) indicate the orientation of the primitive left atrium. Relative to the embryonic plane, the orientation angle progressively increases (approximately 30 deg, 45 deg,