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Faculty of Biology and Biotechnology, Maria Curie-Sklodowska University Courses in English Academic Year: 2013/14 Course title: Microbiology Faculty/Department: Faculty of Biology and Biotechnology, Department of Genetics and Microbiology Course title: Microbiology Course code: MIRWM Teacher: prof. dr hab. Wanda Małek ([email protected]) (48) 81 537 59 76 Lecture/Laboratory: 30 hrs / 60 hrs ECTS credits: 8 Year of study: II (I0), winter semester Educational and professional goals The purpose of microbiology course is to familiarize students with the major groups of prokaryotes, their cell structure, function, metabolism, and the role of microorganisms in the environment as well as biotechnology. The student learns the techniques used to identify, differentiate microorganisms as well as know the basic strategies and methods to combat pathogenic bacteria. Course description: the lectures: Microbiology beginning. The world of microorganisms. The rules of contemporary classification of microorganisms. Prokaryotic cell structure and functions. Endospores and other resting forms of bacteria. Microbial nutrition: requirements for carbon, nitrogen, iron, phosphorus, sulfur, oxygen, hydrogen. Nutritional types of microorganisms. Microbial growth: measurement of cell number and cell mass, the growth in closed and continuous culture systems. Bacterial biofilms. Metabolism: aerobic and anaerobic respirations, fermentations, chemosynthesis, photosynthesis. Control of microorganism growth by physical and chemical agents. Bacteriophages: structure, lytic and lysogenic cycles. Economic and environmental importance of bacteria. the classes: Comparison of different types of bacterial cell wall structure: Gram-negative, Gram-positive, acidresistant (Gram and Ziehl-Neelsen staining methods). Cytology of bacterial cell: the staining methods of cell wall, genetic material, endospores, capsules. The microbiological techniques: isolation of pure cultures, identification of bacteria on the basis of morphological and physiological features. Measure of the bacterial growth. A types of microbiological culture media: preparation, characteristics. Control of microorganisms: sterilization and disinfection. The effect of environmental factors on bacterial growth: temperature, UV, osmotic pressure, pH, antibiotics. Interactions between organisms: mutualism, antagonism. Identification of microorganisms in dairy products. Bacteriophages: isolation, bacteriophage plaque-count assay, host range determination. Literature: “Biology of Microorganisms”- Michael T. Madigan, John M. Martinko, Jack Parker, Prentice Hall International, Inc; “Microbial Life”- Jerome J. Perry, James T. Staley, Stephan Lory, Sinauer Associates, Publisher Sunderland, Massachusetts Assesment method: oral or written exam Prerequisities: basic knowledge of English Primary target group: biologists, biotechnologists 1 Course title: Biochemistry of Secondary Metabolites Faculty/Department: Biochemistry Department, Faculty of Biology and Biotechnology Course title: Biochemistry of secondary metabolites Course code: Erasmus subject area code: Number of contact hours: 30 hours – lecture; 30 hours - laboratory Course duration: I 20, summer semester ECTS credits: 5 Course description: The position of secondary metabolism in biochemical processes in living organisms and its regulation at molecular and environmental level. The course covers the major groups of secondary metabolites in microorganisms and plants, from the perspectives of biochemistry and biosynthesis with references to the relationship between biological function of secondary metabolites in defense against different stress. Characteristics of basic secondary metabolites (bacterial, fungal and from plants), their biosynthetic pathways and practical applications for example such as pharmacological compounds. Literature: Dewick PM “Medicinal natural products. A biosynthetic approach.” John Wiley & Sons, LTD, 2002 Wink M (ed) “Functions and biotechnology of plant secondary metabolites” Wiley-Blackwell, 2010 Course type: lecture and practical laboratory Assesment method: written examination, continuous assessment of laboratories Prerequisities: completed course in biochemistry Primary target group: students of biology, biotechnology, organic chemistry Lecturer: dr hab. Anna Jarosz-Wilkolazka Contact person: Anna Jarosz-Wilkolazka, Email: [email protected] Phone: (48 81) 537 50 44 Deadline for application: Remarks: Educational and professional goals - student knows the main groups of secondary metabolites produced by microorganisms and plants; can indicate a correlation between structure of secondary metabolites such as alkaloids, terpenoids and antibiotics and their biological activities; is aware of chemical and biological diversities of natural environment. Course title: Medical Microbiology Course title: Medical Microbiology Teacher: Urbanik-Sypniewska Teresa, Wdowiak-Wróbel Sylwia (e-mail: [email protected]; [email protected]) Lecture/Laboratory, 30 hrs/ 60 hrs ECTS credits: 8 Year of study 1st of 2º summer semester Educational and professional goals The course provides a basic theoretical and technical study of the structure, molecular biology, pathogenesis, epidemiology, and laboratory identification of the various bacteria that cause human diseases. The student can convert the acquired theoretical background knowledge into actual practice in the course of experiments. University and government laboratories, research institutes, the pharmaceutical and biotechnology industries, and diagnostic laboratories are employers looking for qualified microbiologists. Course description Lecture: Introduction to Medical Microbiology: Normal Flora (human microbiome) Gram-positive cocci: Staphylococcus, Streptococcus, Enterococcus 2 Gram-positive spore-formers: Bacillus, Clostridium Anaerobic infections: Bacteroides, Porphyromonas, Prevotella, Fusobacterium Gram-negative bacilli: Enterobacteriaceae; Yersinia, Salmonella, Shigella, Escherichia, Coliforms, Klebsiella, Proteus Gram-negative bacilli oxidase positive Pseudomonas, Burkholderia, Acinetobacter Gram-negative curved bacilli: Vibrio, Campylobacter, Helicobacter Gram-negative coccobacilli: Brucella, Bordetella Fastidious Gram-negative bacteria: Neisseria, Haemophilus, HACEK group infections, Legionella, Coxiella Animal-associated bacteria: Erysipelotrix, Francisella, Pasteurella, Mannheimia Spirochetes: Treponema, Leptospira, Borrelia Gram-positive bacilli: Listeria, Corynebacteria, Mycobacteria Obligate intracellular bacteria: Rickettsia, Chlamydia Cell Wall-less bacteria: Mycoplasma, Ureaplasma Antimicrobial Chemotherapy Laboratory: Isolation and culturing of bacteria. Microscopic examination of bacterial morphology by different staining methods /Gram, Neisser, Ziehl-Neelsen. Examination and identification of selected groups of pathogenic bacteria by using laboratory detection methods: molecular, morphological, immunological and cultural. Examination of factors affecting disease spread. Detection of antibiotic resistance mechanisms. Biochemical test for identification of bacteria /API-testing/. Preparation of antibiograms. Determination of MIC and MBC. PCR methods for identification of pathogenic strains. Required background: Biology course and/or General Microbiology course Form of assessment: The written test exam composed of test questions e.g. True/False, Matching, Multiple Choice and Short Answer Course title: Plant physiology Faculty/Department: Faculty of Biology and Biotechnology / Department of Plant Physiology Course title: Plant physiology Course code: FRRWM Erasmus subject area code: 13.1 (Biology, plant physiology / electrophysiology) Number of contact hours: 90 (lecture 30 hrs / laboratory 60 hrs) Course duration: summer semester ECTS credits: 8 Course description: Issues for lecture: Water balance in plants, water uptake, transport and transpiration. Mineral nutrition of plants. Structure, organization and function of photosynthetic apparatus, mechanisms and regulation of the CO2 fixation and reduction. Carbohydrate metabolism and cellular respiration. Plant growth and development, the role of phytohormones. Types and mechanisms of plant movements. During laboratory classes students perform practical exercises concerning: water relations in plant cells (diffusion, osmosis), measurement of transpiration intensity, analysis of mineral and organic compounds of plants, isolation and properties of photosynthetic pigments, influence of light intensity and spectrum on photosynthesis, gas exchange during plant respiration, plant growth, seed germination, plant movements (phototropism, geotropism, photonasty). Literature: Biochemistry & Molecular Biology of Plants, B. Buchanan, W . Gruissem, R. Jones (Eds), American Society of Plant Physiologists, 2000. Course type: lecture + laboratory Assesment method: written exam Prerequisities: basic knowledge of English Primary target group: biology/biotechnology students Lecturer: Prof. dr hab. Zbigniew Krupa Contact person: dr Małgorzata Wójcik, email: [email protected], tel. +48 81 5375064 Deadline for application: Remarks: - 3 Course title: Plant physiology (Basic course) Faculty/Department: Faculty of Biology and Biotechnology / Department of Plant Physiology Course title: Plant physiology (Basic course) Course code: FRPAT Erasmus subject area code: 13.1 (Biology, plant physiology / electrophysiology) Number of contact hours: 60 (lecture 30 hrs / laboratory 30 hrs) Course duration: summer semester ECTS credits: 5 Semester:spring Course description: Issues for lecture: Water balance in plants, water uptake, transport and transpiration. Mineral nutrition of plants. Structure, organization and function of photosynthetic apparatus, mechanisms and regulation of the CO2 fixation and reduction. Carbohydrate metabolism and cellular respiration. Plant growth and development, the role of phytohormones. Types and mechanisms of plant movements. During laboratory classes students perform practical exercises concerning: water relations in plant cells (diffusion, osmosis), measurement of transpiration intensity, analysis of mineral and organic compounds of plants, isolation and properties of photosynthetic pigments, influence of light intensity and spectrum on photosynthesis, gas exchange during plant respiration, plant growth, seed germination, plant movements (phototropism, geotropism, photonasty). Literature: Biochemistry & Molecular Biology of Plants, B. Buchanan, W . Gruissem, R. Jones (Eds), American Society of Plant Physiologists, 2000. Course type: lecture + laboratory Assesment method: written exam Prerequisities: basic knowledge of English Primary target group: biology/biotechnology students Lecturer: Prof. dr hab. Zbigniew Krupa Contact person: dr Małgorzata Wójcik, email: [email protected], tel. 81 5375064 Deadline for application: Remarks: - Course title: Mycology Course title: Mycology Teacher: prof. dr hab. Wiesław Mułenko (e-mail: [email protected]) Lecture/Laboratory, 15 hrs / 30 hrs ECTS credits: 3 Year of study: I(Io), winter semester Educational and professional goals: Knowledge of the basic morphological and anatomical features of the fungal structure, distinguishing them from other groups of organisms, and modes of reproduction and propagation in the environment (specialist terminology). Knowledge of the role and importance of fungi in the natural environment; protection of fungi as the natural components of the biosphere. Relationships between fungi and other organisms – parasitism, commensalism, mutualism. Mycorrhiza, endophytes, lichens (lichenized fungi). Ability to identify and distinguish the main edible and poisonous species and identification of symptoms of diseases caused by fungi. Course description Structure of fungi and fungus-like organisms. Specific components of fungal cells. Vegetative and generative fungal structures. Characteristic of selected representatives of Myxomycota, Oomycota, Zygomycota, Ascomycota, Basidiomycota and anamorphic fungi. Morphology and anatomy of fungal fruiting bodies. Types of asexual and sexual reproduction, sporogenesis, spore propagation. Trophic forms. Fungal metabolites and their application. The role and importance of fungi in the ecosystem. Modern criteria for classification of fungi, an overview of major taxa (The Code of Nomenclature). 4 Required background: Form of assessment: Written examination (lecture) and test questions (laboratory) Course title: General and Systematic Botany Course title: General and Systematic Botany Teacher: prof. dr hab. Wiesław Mułenko (e-mail: [email protected]) Lecture/Laboratory: 15 hrs / 60 hrs + 30 hrs (field classes) ECTS credits: 8.5 Year of study: I(Io), summer semester Educational and professional goals Knowledge of the basic morphological and anatomical features of the plant structure, their classification and modifications; knowledge of the role and importance of plants in the natural environment as the key components of the biosphere; plant protection; adaptation to environmental conditions; the role of plants in human life; plant use. Skills to prepare microscope slides of various plant structures. Ability to identify the most important plant species. Efficient use of appropriate equipment and keys for identification of organisms. Course description Plant cell structure. Forms of organization of plant organism. The structure and classification of tissues. Morphology, anatomy, and modifications of vascular plant organs (root, stem, leaves). Flowers and inflorescences – the structure, classification, modes of pollination. Fruits and seeds – development, structure, classification, and propagation modes. Modes of plant propagation, alternation of generations. An overview of major plant systems; the rules of botanical nomenclature, the code of nomenclature. The main hypothesis of the origin and evolution of axial plants and thallophytes. A systematic overview of the main strategies in plant development. An overview of systematic groups with particular emphasis on seed plants as well as protected and crop species. Required background: – Form of assessment: Written examination (lecture) and test questions (laboratory); herbarium (after field exercises). Course title: Microscopic techniques Faculty/Department: Faculty of Biology and Biotechnology, Comparative Anatomy and Anthropology Department Course title: Microscopic techniques Course code: TEMBP Erasmus subject area code: 13.1 Number of contact hours: 30 Course duration: II (II o), summer semester ECTS credits: 3 Course description The exercises enable to earn theoretical and practical knowledge from different kind of light microscopy e.g. fluorescence, dark-field as well as confocal and electron-transmission and scanning. Main topics include: construction of electron and confocal microscope, construction and operation on light microscope-light - , dark-field, fluorescent microscope, preparation of specimens for electron microscopy: trimming, cutting, contrasting of biological samples, grids observation under electron microscope. Literature: Alberts B i in. PWN 2005, Zabel M. Immunocytochemia PWN 1999, Kłyszejko Stefanowicz L. Cytobiochemia PWN 2002, Cieciura L. Techniki stosowane w mikroskopii elektronowej. PWN 1989 Course type: Laboratory: 30hrs Assessment method: Continuous assessment in a form of tests- two tests during the course on which final mark is based 5 Prerequisities: The passed courses from cell biology, chemistry and biochemistry Primary target group: students of biology Lecturer: Pawlikowska-Pawlęga Bożena, PhD, Pawelec Jarek, M.S. Wydrych Jerzy, M.S. Contact person: Pawlikowska-Pawlęga Bożena, PhD ([email protected]), 537-59-28; 537-59-16 Deadline for application: winter semester Remarks: Classes are conducted with application of the sophisticated equipment: Leo– Zeiss 912 AB electron microscope (Oberkohen, Germany), fluorescence microscope NIKON E-800 (Japan), microtome RMC MT – XL (Tucson, AZ, USA), Confocal microscope LSM 5 PASCAL; Educational and professional goals that the student achieves are as follows: Graduate characterizes and explains chosen microscopic techniques, lists and characterizes stages of preparation of biological samples, identifies particular cell organelles of the cells, employs techniques of biological preparation including different microscopic techniques, documents the results of the experiments, Selects and applies microscopic techniques during observation and experiments. Functional Human Anatomy Course title: Functional Human Anatomy Course code: AFRAG Erasmus subject area code: 13.1 Number of contact hours: 30 Course duration: Winter semester ECTS credits: 4 Course description: Microscopic observation of different tissues: epithelial, muscular, connective and nervous. Macro- and microscopic structure of organs creating all the anatomical systems of human body. The topics include: structure ,function and topography of skeletal, muscular, digestive, respiratory, transport, urinary, reproductive, nervous and endocrine systems. Human tissues, elements of anthropology. Literature: Drake, Vogl, Mitchell “Gray’s Anatomy for Students”; Abrahams, Boom, Spratt, Hutchings „Clinical Atlas of Human Anatomy”; Fenneis “Pocket Atlas of Human Anatomy”; Sobotta “Atlas of Human Anatomy”. Course type: laboratory Assessment method: Continuous assessment in a form of written tests - several tests during the course and final written test. Prerequisites: Completed course in cell biology Primary target group: Lecturer: Dobrowolski Piotr PhD Contact person: Dobrowolski Piotr, [email protected], +48 507 132 520 Deadline for application: Remarks: Course title: Basic techniques of cell and tissue culture Faculty/Department: Faculty of Biology and Biotechnology/Department of Plant Anatomy and Cytology Course title: Basic techniques of cell and tissue culture Course code: PHTRP Erasmus subject area code: Number of contact hours: 60 hrs Course duration: Year of study: III (Iº), winter semester ECTS credits: 6 Course description: Plant cell and tissue culture laboratory – basic equipment and organization of work. The main techniques of sterilization and preparation of plant material. Components of culture media and 6 preparation procedures. Hormonal control of cell growth and development. Organogenesis induction and plant regeneration in cultured explants on solid media. Initiation and establishment of callus culture from different types of explants. Meristem culture (isolation of shoot apical meristems). In vitro clonal propagation of crop plants (method of micropropagation from axillary buds). Establishment of cell suspension culture and its application in biotechnology. Literature: Evans D.E., Coleman J.O.D., Kearns A., Plant Cell Culture. BIOS Scientific Publishers, 2003 Smith R.H., Plant Tissue Culture. Techniques and Experiments. Academic Press, Second Edition, 2000 Course type: Laboratory Assessment method: Written test questions Prerequisities: Plant physiology, biochemistry, genetics Primary target group: podstawowa grupa, do której kierowany jest kurs Lecturer: Dr. Ewa Dziadczyk, dr hab. Ewa Szczuka, prof. UMCS Contact person: dr hab. Ewa Szczuka, prof. UMCS, [email protected] Deadline for application: Remarks: Course title: Methods of in vitro tissue culture Faculty/Department: Faculty of Biology and Biotechnology/Department of Plant Anatomy and Cytology Course title: Methods of in vitro tissue culture Course code: MKTBP Erasmus subject area code: Number of contact hours: 30 hrs Course duration: Year of study: II (IIº), winter semester ECTS credits: 3,5 Course description: Basic equipment and organization of work in plant cell and tissue culture laboratory. Components of culture media and preparation procedures. Stages of preparation of sterile culture media; proliferation of cell mass; hormonal development orientation (direct and indirect regeneration); somatic embryogenesis; embryogenesis of haploid plants from microspores.. Hormonal control of cell growth and development. Organogenesis induction and plant regeneration in cultured explants on solid media. Initiation and establishment of callus culture from different types of explants. Meristem culture (isolation of shoot apical meristems). In vitro clonal propagation of crop plants (method of micropropagation from axillary buds). Establishment of cell suspension culture and its application in biotechnology. Micropropagation; protoplast culture; microspore culture. Literature: Evans D.E., Coleman J.O.D., Kearns A., Plant Cell Culture. BIOS Scientific Publishers, 2003 Smith R.H., Plant Tissue Culture. Techniques and Experiments. Academic Press, Second Edition, 2000 Pollard J.W., Walker J.M. Plant Cell and Tissue Culture, Human Press 1990 Course type: Laboratory Assesment method: Written and oral assessment Prerequisities: Plant physiology, biochemistry, genetics Primary target group: Lecturer: dr hab. Ewa Szczuka, prof. UMCS, dr. Ewa Dziadczyk, Contact person: dr hab. Ewa Szczuka, prof. UMCS, [email protected] Deadline for application: Remarks: 7 Course title: Plant Embryology Faculty/Department: Faculty of Biology and Biotechnology/Department of Plant Anatomy and Cytology Course title: Plant Embryology Course code: EMRJB Erasmus subject area code: Number of contact hours: Lecture/Laboratory: 30 hrs /45 hrs Course duration: Year of study: I (IIº), winter semester ECTS credits: 6 Course description: Heterogenic cycle and sex determination in plants. Bryophytes: sporangium and sporogenesis; archegonia, antheridia and gametes. Generative organs of ferns and horsetails. Heterospory in club mosses and water ferns. Gymnosperms: pollen sac development, microsporogenesis, pollen grain development, ovule development, macrosporogenesis, perisperm, archegonia, embryo. Angiosperms: anther development, microsporogenesis, pollen development, types of: ovules, macrosporogenesis and embryo sacs; pollination, progamic phase, fertilization, embryo, seed development. apomixis, GMO Literature: 1. Wang T.L., Cuming A. Embryogenesis the generation of a plant. Bios 20062. Lersten N.R. Flowering Plant Embryology. 2004 Course type: Lecture, Laboratory Assesment method: Test (written examination), continuous assessment of laboratory Prerequisities: Completed course in general and taxonomic botany Primary target group: Lecturer: dr hab. Ewa Szczuka, prof. UMCS Contact person: dr hab. Ewa Szczuka, prof. UMCS ([email protected]) Deadline for application: Remarks: Molecular genetics Name of course: Molecular genetics Teacher: prof. dr hab. Teresa Jakubowicz ([email protected]) Lecture: 30 hrs ECTS credits: 2 Year of study: II (II0 ), winter semester Educational and professional goals: The aim of the course is to introduce and discuss current topics related to molecular genetics of eukaryotic organisms. Course description: Comparative genomics. Mechanisms of pre-RNA processing in eukaryotic cells. Types of splicing, editing, catalytic RNA and DNA. Noncoding RNAs - biogenesis, physiological and pathological role. RNA interference in vivo and therapeutic potential of RNAi. Epigenetic inheritance, epigenetic mechanisms in human diseases. Genetic control of differentiation and development. Homeotic genes. Cancer genetics. Chromatin regulation at mammalian telomers. Required background: Completed courses of biochemistry (or molecular biology), genetics Form of assessment: Written examination Molecular mechanisms of adaptation Name of course: Molecular mechanisms of adaptation Teacher: prof. dr hab. Teresa Jakubowicz ([email protected]), dr hab. Małgorzata cytrynska ([email protected]), dr hab. Iwona Wojda ([email protected]) Lecture/laboratory: 30hrs / 45 hrs 8 ECTS credits: 6,5 Educational and professional goals: The aim of the course is to give students comprehensive view of molecular mechanisms of eukaryotic organisms adaptations. Course description: Gene expression in humans and animals regulated by changes in environmental conditions. Gene and genome rearrangement. Adaptive genes. Methods used for organisms changeability determination. Adaptive character of immune response. Required background: Completed course in genetics Form of assessment:: Written examination Course title; Invertebrate immunology Name of course: Invertebrate immunology Teacher: dr hab. Małgorzata Cytryńska ([email protected]) Lecture/Laboratory: 15 hrs/25 hrs ECTS credits: 3.5 Year of study: I (IIo), summer semester Educational and professional goals Knowledge of essential mechanisms of invertebrate immunity. Understanding of differences between vertebrate and invertebrate immune response. Course description Essential features of invertebrate immunity on the example of insect innate immunity. Self-non-self recognition. Mechanisms of invertebrate immunity: anatomical and physiological barriers; cellular response (types of hemocytes, phagocytosis, nodulation, encapsulation); humoral response (lysozyme, phenoloxidase system, defense peptides and proteins). Regulation of defense peptide gene expression in Drosophila. Immunoglobulin superfamily in invertebrates. Entomopathogenic organisms. Bioinsecticides. Required background; Biochemistry, microbiology Form of assessment: Written examination 9